Lycopene |
| Catalog No.GN10448 |
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
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Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
PC3 cells and MDA-MB-231 cells are treated with (0, 0.5, 1.25, 2.5 and 5 μM) lycopene for 48 h. Cell survival/growth is measured using the colorimetric MTS assay method. MTS-PMS complex (20 μL) is added to each well. The catalytic activity of viable cells results in formazan dye production, which is then quantified. Cells are incubated with the dye for 1 h, followed by absorbance reading at 492 nm on a spectrophotometer[1]. |
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Animal experiment: |
Rats: Lycopene is dissolved in corn oil. 35 adult male albino rats are randomized into five equal groups: untreated control, vehicle control (receives 0.5 mL corn oil/day), lycopene (10 mg/kg b.w./day), ATZ (dissolved in 0.5 mL corn oil 300 mg/kg b.w./day), and ATZ + lycopene. All treatments are given by oral gavage for 4 weeks[4]. |
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References: [1]. Assar EA, et al. Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells. Tumour Biol. 2016 Jul;37(7):9375-85. |
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Lycopene is naturally occurring carotenoids found in tomato, tomato products, and in other red fruits and vegetables; exhibits antioxidant effects.
Sufficient uptake of lycopene from the diet is necessary to benefit from its health promoting effects, since humans are unable to synthesise lycopene de novo. Lycopene significantly inhibits prostate and breast cancer cell growth at physiologically relevant concentrations of 1.25 μM and also causes a 30-40 % reduction in inhibitor of kappa B phosphorylation in the cells[1]. Increased intake of lycopene, a major carotenoid in tomatoes, consumed as the all-trans-isomer attenuates alcohol induced apoptosis in 2E1 cells and reduces risk of prostate, lung and digestive cancers. Lycopene plays a role in the protection against photooxidative processes by acting as singlet molecular oxygen and peroxyl radicals scavengers and can interact synergistically with other antioxidants[2]. Lycopene as a carotenoid can react with types of reactive oxygen species (ROS) in three different mechanisms: I) by electron-transfer, II) by hydrogen atom transfer or III) by adduct formation. Lycopene is able to deactivate singlet oxygen mainly by physical quenching[3]. Lycopene decreases ROS production in SK-Hep-1 cells through inhibition of NADPH oxidase, brought about in the PKC pathway[5].
Lycopene is the most predominant carotenoid in human plasma and has a half life of about 2-3 days[2]. Lycopene or processed tomatoes may lead to a reduction of intima-media thickness in vessel walls[3]. Lycopene exerts protective effects against ATZ-induced toxicity in rat adrenal cortex. These effects may be attributed to the antioxidative property of lycopene and its ability to activate the Nrf2/HO-1 pathway[4]. Lycopene improves hepatotoxicity acting as an antioxidant, reduces GSSG and regulates tGSH and CAT levels, reduces oxidative damage[5].
References:
[1]. Assar EA, et al. Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells. Tumour Biol. 2016 Jul;37(7):9375-85.
[2]. Tapiero H, et al. The role of carotenoids in the prevention of human pathologies. Biomed Pharmacother. 2004 Mar;58(2):100-10.
[3]. Müller L, et al. Lycopene and Its Antioxidant Role in the Prevention of Cardiovascular Diseases-A Critical Review. Crit Rev Food Sci Nutr. 2016 Aug 17;56(11):1868-79.
[4]. Abass MA, et al. Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway. Environ Sci Pollut Res Int. 2016 Aug;23(15):15262-74.
[5]. Bandeira AC, et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice. Chem Biol Interact. 2017 Feb 1;263:7-17.
| Cas No. | 502-65-8 | SDF | |
| Synonyms | N/A | ||
| Chemical Name | (6E,8E,10E,12E,14E,16E,18E,20E,22E,24E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,8,10,12,14,16,18,20,22,24,26,30-tridecaene | ||
| Canonical SMILES | CC(=CCCC(=CC=CC(=CC=CC(=CC=CC=C(C)C=CC=C(C)C=CC=C(C)CCC=C(C)C)C)C)C)C | ||
| Formula | C40H56 | M.Wt | 536.89 |
| Solubility | <3.03 mg/mL in 0.5MNAOH | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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