Lycopene (Synonyms: NSC 407322) |
Catalog No.GN10448
One-Click Copy Product Info
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Lycopene is a fat-soluble carotenoid molecule that is found in high concentrations in red fruits and vegetables and has a strong antioxidant effect.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 502-65-8
Sample solution is provided at 25 µL, 10mM.
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Lycopene is a fat-soluble carotenoid molecule that is found in high concentrations in red fruits and vegetables and has a strong antioxidant effect [1]. Lycopene can inhibit the activity of HMG-CoA reductase[2]. Lycopene can induce G0/G1 arrest and S phase arrest of the cell cycle[3].
In vitro, Lycopene (1-5μM) treatment of HT-29, T84, and MCF-7 cells for 48h significantly reduced the number of viable cells, with a 30% reduction in HT-29 and T84 cells and a 10% reduction in MCF-7 cells, and also caused cell cycle arrest at different stages [4]. Lycopene (0.5-10μM) treatment of SK-Hep-1 cells reduced the production of intracellular ROS by inhibiting the NADPH oxidase produced in the PKC pathway [5].
In vivo, oral treatment of mice inoculated with SK-Hep-1 tumor cells with Lycopene (20 mg/kg) for 12 weeks significantly reduced the average number of tumors and tumor cross-sectional area, and also reduced the positive rate of proliferating cell nuclear antigen (PCNA) in lung tissue, as well as the expression of PCNA, MMP-9 and VEGF proteins [6]. Oral treatment of diabetic rats with Lycopene (10 mg/kg) significantly reduced the activity of angiotensin-converting enzyme (ACE) in plasma and heart tissue [7].
References:
[1] Wang Q, Wu X, Zhang J, et al. Role of ROS/JAK2/STAT3 signaling pathway in di-n-butyl phthalate-induced testosterone synthesis inhibition and antagonism of lycopene[J]. Food and Chemical Toxicology, 2023, 175: 113741.
[2] Palozza P, Catalano A, Simone R E, et al. Effect of lycopene and tomato products on cholesterol metabolism[J]. Annals of Nutrition and Metabolism, 2012, 61(2): 126-134.
[3] Hwang E S, Bowen P E. Cell cycle arrest and induction of apoptosis by lycopene in LNCaP human prostate cancer cells[J]. Journal of medicinal food, 2004, 7(3): 284-289.
[4] Teodoro A J, Oliveira F L, Martins N B, et al. Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines[J]. Cancer cell international, 2012, 12: 1-9.
[5] Bandeira A C B, da Silva T P, de Araujo G R, et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice[J]. Chemico-Biological Interactions, 2017, 263: 7-17.
[6] Huang C S, Liao J W, Hu M L. Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice[J]. The Journal of nutrition, 2008, 138(3): 538-543.
[7] Ozmutlu S, Dede S, Ceylan E. The effect of lycopene treatment on ACE activity in rats with experimental diabetes[J]. Journal of the Renin-Angiotensin-Aldosterone System, 2012, 13(3): 328-333.
| Cell experiment [1]: | |
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Cell lines |
HT-29、T84、MCF-7 cells |
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Preparation Method |
Three cancer cells lines (HT-29, T84 and MCF-7) were treated with lycopene (1–5 μM) for 48 and 96 h. Cell viability was monitored using the method of MTT. |
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Reaction Conditions |
1-5μM; 48、96h |
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Applications |
After treatment for 48 h, a significant decrease in the number of viable cells was observed in three cancer cells lines as compared to controls, reaching 30% reduction in HT-29 and T84 cells and 10% in MCF-7 cells. |
| Animal experiment [2]: | |
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Animal models |
Adult male athymic nude mice |
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Preparation Method |
Mice were orally administered with lycopene (1 and 20 mg/kg) or β-carotene (20 mg/kg) in corn oil twice per week for 12 wk. Corn oil alone was given to the noninjected control mice. Two weeks into the supplementation, the mice were injected once with SK-Hep-1 cells into the lateral tail vein and blood samples were drawn once per week from the retro-orbital plexus for the measurement of MMP and VEGF. |
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Dosage form |
1, 20mg/kg; p.o. |
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Applications |
High lycopene supplementation significantly reduced the mean number of tumors and the cross-sectional area of tumors. High lycopene supplementation also decreased the positive rate of proliferating cellular nuclear antigen (PCNA), and protein expressions of PCNA, MMP-9, and VEGF in lung tissues. |
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References: [1] Teodoro A J, Oliveira F L, Martins N B, et al. Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines[J]. Cancer cell international, 2012, 12: 1-9. [2]Huang C S, Liao J W, Hu M L. Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice[J]. The Journal of nutrition, 2008, 138(3): 538-543. |
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| Cas No. | 502-65-8 | SDF | |
| Synonyms | NSC 407322 | ||
| Chemical Name | (6E,8E,10E,12E,14E,16E,18E,20E,22E,24E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,8,10,12,14,16,18,20,22,24,26,30-tridecaene | ||
| Canonical SMILES | CC(=CCCC(=CC=CC(=CC=CC(=CC=CC=C(C)C=CC=C(C)C=CC=C(C)CCC=C(C)C)C)C)C)C | ||
| Formula | C40H56 | M.Wt | 536.89 |
| Solubility | <3.03 mg/mL in 0.5MNAOH; DMSO:12mg/ml | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8626 mL | 9.3129 mL | 18.6258 mL |
| 5 mM | 372.5 μL | 1.8626 mL | 3.7252 mL |
| 10 mM | 186.3 μL | 931.3 μL | 1.8626 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)