GLPBio

HSF1A

Catalog No.: GC32437Purity:>99.00%
COASDSData Sheet
HSF1A is an activator of Heat Shock Transcription Factor 1 (HSF1).

Products are for research use only. Not for human use. We do not sell to patients.

HSF1A
Cas No.: 1196723-93-9
10mM (in 1mL DMSO)In Stock
$95.00
1
2mgIn Stock
$70.00
1
5mgIn Stock
$88.00
1
10mgIn Stock
$126.00
1
25mgIn Stock
$273.00
1
50mgIn Stock
$392.00
1
100mgIn Stock
$553.00
1
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Citations

2 publicationsPublications from researchers using this product

Description

HSF1A is an activator of Heat Shock Transcription Factor 1 (HSF1) [1]. HSF1A inhibits the activity of the molecular chaperone TRiC/CCT, thereby activating HSF1 and enhancing cellular resistance to protein misfolding and stress [2-3]. HSF1A is primarily used to treat neurodegenerative diseases [4].

In CD150+CD48-LSK hematopoietic stem cells (HSCs), HSF1A (8μM; 10d) enhances the serial reconstitutive activity of HSCs cultured in vitro [5]. In chinese hamster ovary cells, HSF1A (200µM; 1h) protects cells from pertussis toxin-induced cytotoxicity [6].

In wistar kyoto rats, HSF1A (100mg/kg; ip; 4 weeks) ameliorates doxorubicin-induced heart failure in vivo [7]. In ovalbumininduced allergic asthma mice model, HSF1A (1.3g/kg; ig; 5 weeks) treatment not only reduces inflammation and oxidative stress but also significantly attenuates airway fibrosis [8].

References:
[1]. Neef D W, Jaeger A M, Gomez-Pastor R, et al. A direct regulatory interaction between chaperonin TRiC and stress-responsive transcription factor HSF1[J]. Cell reports, 2014, 9(3): 955-966.
[2]. Neef D W, Turski M L, Thiele D J. Modulation of heat shock transcription factor 1 as a therapeutic target for small molecule intervention in neurodegenerative disease[J]. PLoS biology, 2010, 8(1): e1000291.
[3]. Steinemann M, Schlosser A, Jank T, et al. The chaperonin TRiC/CCT is essential for the action of bacterial glycosylating protein toxins like Clostridium difficile toxins A and B[J]. Proceedings of the National Academy of Sciences, 2018, 115(38): 9580-9585.
[4]. Liu A Y, Minetti C A, Remeta D P, et al. HSF1, aging, and neurodegeneration[J]. Cell Biology and Translational Medicine, Volume 18: Tissue Differentiation, Repair in Health and Disease, 2022: 23-49.
[5]. Kruta M, Sunshine M J, Chua B A, et al. Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and aging[J]. Cell stem cell, 2021, 28(11): 1950-1965. e6.
[6]. Jia J, Zoeschg M, Barth H, et al. The chaperonin TRiC/CCT inhibitor HSF1A protects cells from intoxication with pertussis toxin[J]. Toxins, 2024, 16(1): 36.
[7]. Huang C Y, Kuo W W, Lo J F, et al. Doxorubicin attenuates CHIP-guarded HSF1 nuclear translocation and protein stability to trigger IGF-IIR-dependent cardiomyocyte death[J]. Cell death & disease, 2016, 7(11): e2455-e2455.
[8]. Liu X, Zhang Y, Wu H, et al. HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2—Dectin-1/SYK mediated ROS and inflammatory responses[J]. Scientific Reports, 2025, 15(1): 29741.

Protocol

Cell experiment [1]:

Cell lines

CD150+CD48-LSK hematopoietic stem cells (HSCs)

Preparation Method

CD150+CD48-LSK HSCs were sorted from the bone marrow of young adult mice directly into 96-well v-bottom plates at a density of 10 cells/well containing freshly made basic HSC culture medium (Prime-XV Mouse Hematopoietic Cell Medium) with SCF (50ng/ml), TPO (50ng/ml), 2-Mercaptoethanol (50μM) and bovine serum albumin (BSA; 0.1%). When specified, basic HSC medium was supplemented with 17-AAG (5nM) or HSF1A (8μM) in 0.02% DMSO. As a control, basic HSC medium was supplemented with 0.02% DMSO.

Reaction Conditions

8μM; 10d

Applications

HSF1A enhances the serial reconstitutive activity of HSCs cultured in vitro.
Animal experiment [2]:

Animal models

Wistar Kyoto rats

Preparation Method

Ten-week-old Wistar Kyoto (WKY) rats were used. Rats were housed at a constant temperature (22℃) with a 12-hour light/dark cycle and provided with food and tap water. The animals were divided into three groups: WKY rats (control group), Doxorubicin (DOX) rats, and DOX rats treated with HSF1A. Each group contained five animals. The DOX group received intraperitoneal injections of DOX (5mg/kg) for 6 consecutive weeks, reaching a cumulative dose of 30mg/kg, which has been shown to be cardiotoxic. HSF1A, a small molecule HSF1 activator, was also administered intraperitoneally (100mg/kg/day).

Dosage form

100mg/kg; ip; 4 weeks

Applications

HSF1A ameliorates doxorubicin-induced heart failure in vivo.

References:
[1]. Kruta M, Sunshine M J, Chua B A, et al. Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and aging[J]. Cell stem cell, 2021, 28(11): 1950-1965. e6.
[2]. Huang C Y, Kuo W W, Lo J F, et al. Doxorubicin attenuates CHIP-guarded HSF1 nuclear translocation and protein stability to trigger IGF-IIR-dependent cardiomyocyte death[J]. Cell death & disease, 2016, 7(11): e2455-e2455.

Product Documents

Purity:>99.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
1196723-93-9
SMILES
O=S(C1=CC=C(CC)C=C1)(NC2=CC(C3=CC=CS3)=NN2C4=CC=CC=C4)=O
Molecular Formula
C21H19N3O2S2
Molecular Weight
409.52
Solubility
DMSO : ≥ 150 mg/mL (366.28 mM);Water : < 0.1 mg/mL (insoluble)
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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