Humanin
Catalog No.: GC18324Purity:>98.00%
Humanin is a mitochondrial-derived peptide with cytoprotective properties.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 330936-69-1
1mgIn Stock
$80.00
1
5mgIn Stock
$160.00
1
10mgIn Stock
$270.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 1mg | $80.00 | In Stock | 1 | |
| 5mg | $160.00 | In Stock | 1 | |
| 10mg | $270.00 | In Stock | 1 |
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Citations
1 publicationsPublications from researchers using this productDescription
Humanin is a mitochondrial-derived peptide with cytoprotective properties [1]. Humanin interacts with IGFBP-3 and Bax, counteracts the pro-apoptotic activity, and prevents the translocation and activation of Bax, as well as the subsequent release of cytochrome c into the cytoplasm[2]. Humanin has been widely used to promote the survival of cells under stress and aging conditions[3].
In vitro, Humanin treatment (10µM) for 6 days significantly inhibited the death of human cerebral vascular smooth muscle cells (HCSMCs) induced by pathogenic Aβ and increased the level of α-myosin[4]. Humanin (10µg/ml) treatment for 24 hours protects human retinal pigment epithelial cells (hRPECs) from oxidative stress-induced cell death, reduces reactive oxygen species (ROS) production and restores mitochondrial function [5]. Treatment with Humanin (20µM) for 24 hours activates the TGFβ signaling pathway in 1123 cells and promotes cell adhesion[6].
In vivo, Humanin treatment via intraperitoneal injection at a dose of 4mg/kg, every two weeks, for 10 months significantly improved cognitive function and reduced inflammatory responses in aged mice[7]. Daily intraperitoneal injection of a 4mg/kg dose of Humanin for 15 days significantly reduced the serum glucose level of diabetic mice and decreased the levels of caspase-3 and caspase-9 in the hippocampus and prefrontal cortex of diabetic mice[8].
References:
[1] Coradduzza D, Congiargiu A, Chen Z, et al. Humanin and its pathophysiological roles in aging: A systematic review[J]. Biology, 2023, 12(4): 558.
[2] Lee C, Yen K, Cohen P. Humanin: a harbinger of mitochondrial-derived peptides?[J]. Trends in Endocrinology & Metabolism, 2013, 24(5): 222-228.
[3] Rochette L, Meloux A, Zeller M, et al. Role of humanin, a mitochondrial-derived peptide, in cardiovascular disorders[J]. Archives of cardiovascular diseases, 2020, 113(8-9): 564-571.
[4] Jung S S, Van Nostrand W E. Humanin rescues human cerebrovascular smooth muscle cells from Aβ‐induced toxicity[J]. Journal of neurochemistry, 2003, 84(2): 266-272.
[5] Sreekumar P G, Ishikawa K, Spee C, et al. The mitochondrial-derived peptide humanin protects RPE cells from oxidative stress, senescence, and mitochondrial dysfunction[J]. Investigative ophthalmology & visual science, 2016, 57(3): 1238-1253.
[6] Ha C P, Hua T N M, Vo V T A, et al. Humanin activates integrin αV–TGFβ axis and leads to glioblastoma progression[J]. Cell Death & Disease, 2024, 15(6): 464.
[7] Yen K, Wan J, Mehta H H, et al. Humanin prevents age-related cognitive decline in mice and is associated with improved cognitive age in humans[J]. Scientific reports, 2018, 8(1): 14212.
[8] Bulut F, Adam M, Özgen A, et al. Protective effects of chronic humanin treatment in mice with diabetic encephalopathy: a focus on oxidative stress, inflammation, and apoptosis[J]. Behavioural brain research, 2023, 452: 114584.
Protocol
| Cell experiment [1]: | |
Cell lines | Human cerebrovascular smooth muscle cells (HCSMCs) |
Preparation Method | HCSMCs were cultured in DMEM medium supplemented with 10% fetal bovine serum, 1mM non-essential amino acids, 2mM glutamine, 100U/ml penicillin, and 100μg/ml streptomycin at 37°C with 5% CO2 and 95% saturated atmospheric humidity. Cells were seeded into 24-well microplates at a density of 1×103 cells/ml for 24h. Various concentrations of Humanin (0, 0.1, 1, 5, and 10µM) were added to each well. After 6 days of incubation in the presence of beta-amyloid (25µM), cell viability was analyzed. |
Reaction Conditions | 0, 0.1, 1, 5, and 10µM; 6 days |
Applications | Humanin treatment significantly enhanced the cell viability of HCSMCs in the presence of beta-amyloid in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male Balb/C mice |
Preparation Method | Male Balb/C mice were housed under standard laboratory conditions on a 12-hour light/dark cycle (lights on at 7:00 am), constant temperature (23±2°C), and constant humidity (60±5), ad libitum food and water. After 7 days of acclimatization, the mice were randomly divided into four groups: Control, Humanin (4mg/kg), Streptozotocin (STZ), STZ + Humanin (4mg/kg). Each group, comprising ten mice, received daily intraperitoneal injections (i.p.) for a duration of 15 days between 09:00 and 10:00. Collect the blood of mice for analysis. |
Dosage form | 4mg/kg/day; 15 days; i.p. |
Applications | Humanin treatment reduced the serum glucose levels in mice with STZ-induced diabetes. |
References:
[1] Jung S S, Van Nostrand W E. Humanin rescues human cerebrovascular smooth muscle cells from Aβ‐induced toxicity[J]. Journal of neurochemistry, 2003, 84(2): 266-272.
[2] Bulut F, Adam M, Özgen A, et al. Protective effects of chronic humanin treatment in mice with diabetic encephalopathy: a focus on oxidative stress, inflammation, and apoptosis[J]. Behavioural brain research, 2023, 452: 114584.
Product Documents
Purity:>98.00%
Chemical Properties
CAS No.
330936-69-1
Chemical Name
L-methionyl-L-alanyl-L-prolyl-L-arginylglycyl-L-phenylalanyl-L-seryl-L-cysteinyl-L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-threonyl-L-seryl-L-α-glutamyl-L-isoleucyl-L-α-aspartyl-L-leucyl-L-prolyl-L-valyl-L-lysyl-L-arginyl-L-arginyl-L-alanine
SMILES
N/A
Molecular Formula
C119H204N34O32S2
Molecular Weight
2687.2
Solubility
DMSO : 100 mg/mL (37.21 mM; Need ultrasonic)
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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