FiVe1 is a potent vimentin (VIM; the intermediate filament and mesenchymal marker) inhibitor. FiVe1 promotes VIM disorganization and phosphorylation during metaphase, leading to mitotic catastrophe, multinucleation, and the loss of stemness. FiVe1 selectively and irreversibly inhibits the growth of multiple FOXC2-expressing breast cancer cell lines (HMLER cells (IC50: 267 nM); FOXC2-HMLER cells (IC50: 447 nM). FiVe1 inhibits the migration of mesenchymally transformed cells.
References:
[1]. Michael J Bollong, et al. A vimentin binding small molecule leads to mitotic disruption in mesenchymal cancers. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9903-E9912.