Latrunculin B is a cell-permeable actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin and thereby inhibit actin polymerization.Latrunculin B regulates pulmonary vein electrophysiological characteristics and attenuates stretch-induced arrhythmogenesis [1].
Latrunculin B (0-10μM; 60min) treatment of CH27 cells depolymerized actin filaments. Treatment of cells with Latrunculin B (0.1μM; 30min) reduced ghost pen cyclic peptide staining and resulted in changes in the cellular distribution of BCR [2].Latrunculin B (100nM; 30min) decreases the late Na+ current, L-type Ca2+ current, Na+/Ca2+ exchanger current, and stretch-activated BKCa current in PV cardiomyocytes [3]. Latrunculin B displays growth inhibition of HeLa cells with an IC50 value of 1.4μM [4].
Latrunculin B (0.5μM/day; ip; 2months) restored the frequency and number of FO B cells in Rictor KO mice compared with Rictor KO mice treated with vector only, but had no effect on MZ B cell formation, and also restored the levels of pY or pBtk in Rictor KO mice [5]. Treatment of eggs with latrunculin B (5, 10 and 20μg/ml; 90min) resulted in a decrease in the incidence of fertilization [6].
References:
[1] Youssef D T A, Asfour H Z, Genta-Jouve G, et al. Magnificines A and B, antimicrobial marine alkaloids featuring a tetrahydrooxazolo [3, 2-a] azepine-2, 5 (3H, 6H)-dione backbone from the Red Sea sponge Negombata magnifica[J]. Marine Drugs, 2021, 19(4): 214.
[2] Brown BK, Song W. The actin cytoskeleton is required for the trafficking of the B cell antigen receptor to the late endosomes. Traffic. 2001 Jun;2(6):414-27.
[3]Lu Y Y, Lin Y K, Wen Z H, et al. Latrunculin B modulates electrophysiological characteristics and arrhythmogenesis in pulmonary vein cardiomyocytes[J]. Clinical Science, 2016, 130(9): 721-732.
[4] oussef DTA, Asfour HZ, Genta-Jouve G, Shaala LA. Magnificines A and B, Antimicrobial Marine Alkaloids Featuring a Tetrahydrooxazolo[3,2-a]azepine-2,5(3H,6H)-dione Backbone from the Red Sea Sponge Negombata magnifica. Mar Drugs. 2021 Apr 12;19(4):214.
[5] Huang L, Zhang Y, Xu C, Gu X, Niu L, Wang J, Sun X, Bai X, Xuan X, Li Q, Shi C, Yu B, Miller H, Yang G, Westerberg LS, Liu W, Song W, Zhao X, Liu C. Rictor positively regulates B cell receptor signaling by modulating actin reorganization via ezrin. PLoS Biol. 2017 Aug 18;15(8):e2001750.
[6] McAvey BA, Wortzman GB, Williams CJ, Evans JP. Involvement of calcium signaling and the actin cytoskeleton in the membrane block to polyspermy in mouse eggs. Biol Reprod. 2002 Oct;67(4):1342-52.