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Hydrocortisone (Synonyms: Cortisol, Dihydrocostisone, NSC 10483)

Catalog No.GC11551

Hydrocortisone, a natural glucocorticoid secreted by adrenal and extra-adrenal tissues

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Hydrocortisone Chemical Structure

Cas No.: 50-23-7

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10mM (in 1mL DMSO)
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500mg
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1g
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5g
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

The human umbilical cord vein cell line EA.hy.926

Preparation Method

DHA at 0.1, 1 and 10µM, hydrocortisone at 0.5µg/mL or vehicle control. After 24h incubation with the pre-treatment, cells were challenged with recombinant human TNFα (50ng/ml) and IL-1β (10ng/mL).

Reaction Conditions

0.5µg/mL for 24 hours

Applications

In the same inflammatory conditions, pre-treatment with hydrocortisone induced a return to baseline, whereas DHA induced an increase of DHA-derived oxylipins that remains subtle at 0,1 and 1 µM, to reach more than half of the total oxylipin output at 10 µM.

Animal experiment [2]:

Animal models

C57BL/6 mice

Preparation Method

In the hydrocortisone group, mice were subcutaneously injected with 1.5 mg/kg hydrocortisone at 1 h after sepsis induction.

Dosage form

Subcutaneously injection, 1.5 mg/kg

Applications

Model mice were treated with hydrocortisone at 1 h after sepsis induction. After 12 h, the kidney tissue and blood samples were collected. After CLP, the kidney function biomarkers SCR and BUN were significantly elevated in the blood sample of mice, but this elevation was blocked by hydrocortisone treatment.

References:

[1]: A.C.Mottaa. K.Strassburg. P.Oranjea, R.J.Vreekenbc, D.M.Jacobsa, et al. Oxylipin profiling in endothelial cells in vitro - Effects of DHA and hydrocortisone upon an inflammatory challenge. 2019. Prostaglandins & Other Lipid Mediators. 144.doi. org/10.1016/j.prostaglandins.2019.106352
[2]: L Jin, W Liao, X Zhou, Y Wang, J Qian, et al. Hydrocortisone alleviates sepsis-induced acute kidney injury through HSF-1-mediated transcriptional suppression of XPO1. Tissue and Cell. 2022. 79. doi.org/10.1016/j.tice.2022.101915.

Background

Hydrocortisone, a natural glucocorticoid secreted by adrenal and extra-adrenal tissues, locally governs the transcription of genes involved in inflammation, immune response, metabolism, and energy homeostasis via binding to its cognate glucocorticoid receptor (GR) [1]. Glucocorticoids as hydrocortisone and methylprednisolone are known as ABCB1 substrates [2].

Hydrocortisone was observed to significantly decrease co-culture-induced pp38 MAPK expression in bothpancreatic acinar cells (PACs) and stellate cells (PSCs). The 10 nM concentration of hydrocortisone used in the study was significantly lower than the lowest normal serum cortisol level of approximately 140 nM in human sera [3].

The Hydrocortisone treatment alleviates sepsis-induced acute kidney injury in mice through HSF-1-mediated transcriptional suppression of XPO1 [4]. Hydrocortisone (70, 140 or 200 mg/kg) treated mice with i.p. injection of did not change either number or percentage of bone marrow B cell subsets. After 24 h of a single i.p. injection of 140 mg/kg of hydrocortisone a reduction in thymus weight and number of thymocytes could be observed. When mice were i.p. injected with hydrocortisone for three consecutive days, there was a depletion of the percentage and number of progenitors and lymphocytes [2].

References:
[1]. Sridharan, K., Rathore, B., Yousuf, M., Reddy Rachamalla, H. K., Jinka, S., Jaggarapu, M. M. C. S., & Banerjee, R. (2021). Self-assembling derivative of hydrocortisone as glucocorticoid receptor-targeted nanotherapeutics for synergistic, combination therapy against colorectal tumor. Molecular Pharmaceutics, 18(3), 1208- 1228.
[2]. Costa K.M.D, Valente R.C, Silva J.M.C.D, Paiva L.S, Rumjanek V.M, Glucocorticoid susceptibility and in vivo ABCB1 activity differ in murine B cell subsets. An. Acad. Bras. Cienc. 2018; 90 (30304236): 3081-3097. 10.1590/0001-3765201820180364
[3]. BlÄuer, M.; Sand, J.; Laukkarinen, J. Regulation of p38 MAPK and glucocorticoid receptor activation by hydrocortisone in mono-and co-cultured pancreatic acinar and stellate cells. Pancreatology 2021, 21, 384-389.
[4]. L Jin, W Liao, X Zhou, Y Wang, J Qian, et al. Hydrocortisone alleviates sepsis-induced acute kidney injury through HSF-1-mediated transcriptional suppression of XPO1. Tissue and Cell. 2022. 79. doi.org/10.1016/j.tice.2022.101915.

Chemical Properties

Cas No. 50-23-7 SDF
Synonyms Cortisol, Dihydrocostisone, NSC 10483
Chemical Name (8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
Canonical SMILES CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O
Formula C21H30O5 M.Wt 362.46
Solubility ≥ 13.3mg/mL in DMSO Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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Immediate-release granule formulation of hydrocortisone, Alkindi?, for treatment of paediatric adrenal insufficiency (Infacort development programme)

Treatment of paediatric patients with adrenal insufficiency is challenging due to the lack of appropriate glucocorticoid preparations for children, and the use of either pharmacy- or parent-compounded hydrocortisone tablets. Alkindi (hydrocortisone granules in capsules for opening) is a new therapeutic option for paediatric adrenal insufficiency. Areas Covered: Drawbacks of current therapy and formulation and clinical trial programme for Alkindi. Expert Commentary: Compounding hydrocortisone has multiple issues including inconsistent dosing with under and over treatment and practical problems for parents who compound the drug themselves or travel long distances to a compounding pharmacy and the cost of compounding by the pharmacy. Alkindi? is a novel paediatric formulation of immediate release hydrocortisone licensed for use in paediatric adrenal insufficiency. Alkindi? is formulated to address the needs of neonates, infants and young children, being available at appropriate paediatric doses of 0.5, 1.0, 2.0 and 5.0 mg, is multiparticulate, allowing either direct oral dosing or dosing mixed with food, is taste masked to obscure the bitter taste of hydrocortisone and is bioequivalent to current hydrocortisone preparations. Clinical trials in young children with adrenal insufficiency demonstrated cortisol levels after dosing similar to those seen in healthy children and the drug was well tolerated and favoured over current therapy by parents. Alkindi? will provide a licenced treatment option for accurate dosing in children with adrenal insufficiency where compounded adult tablets of hydrocortisone are unsuitable.

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Average Rating: 5 ★★★★★ (Based on Reviews and 19 reference(s) in Google Scholar.)

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