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MTSEA (Synonyms: 2-Aminoethyl methanethiosulfonate, Methanethiosulfonate Ethylammonium)

Catalog No.GC44252

MTSEA is a sulfhydryl-reactive compound that modifies free cysteine residues to produce a positively charged side chain approximately the size of lysine.

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MTSEA Chemical Structure

Cas No.: 16599-33-0

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10mg
$38.00
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50mg
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100mg
$227.00
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250mg
$322.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

MTSEA [(2-aminoethyl) methanethiosulfonate] is a cysteine modifying agent [1], cysteine's sulfhydral side chain (-SH) can form a covalent disulfide bond with sulfur in the MTSEA reagent [2].

MTSEA was used to demonstrate the presence of a water-accessible cysteine within the binding-site crevice of the human dopamine D2 receptor [3]. MTSEA was demonstrated rotation of TM6 (upon receptor activation) by the appearance of an MTSEA-accessible cysteine in a constitutively active mutant of the β2-adrenergic receptor not accessible in the wild-type receptor [4]. The effect of MTSEA on ligand binding, in conjunction with site-directed mutagenesis, was used to define intramolecular contacts in the neurokinin-2 tachykinin receptor [5].

References:
[1]. Choi YB, Tenneti L, Le DA, Ortiz J, Bai G, Chen HS, Lipton SA. Molecular basis of NMDA receptor-coupled ion channel modulation by S-nitrosylation. Nature neuroscience. 2000 Jan;3(1):15-21.
[2]. O'Reilly JP, Shockett PE. Time-and state-dependent effects of methanethiosulfonate ethylammonium (MTSEA) exposure differ between heart and skeletal muscle voltage-gated Na+ channels. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2012 Mar 1;1818(3):443-7.
[3]. Javitch JA, Li X, Kaback J, Karlin A. A cysteine residue in the third membrane-spanning segment of the human D2 dopamine receptor is exposed in the binding-site crevice. Proceedings of the National Academy of Sciences. 1994 Oct 25;91(22):10355-9.
[4]. Rasmussen SG, Jensen AD, Liapakis G, Ghanouni P, Javitch JA, Gether U. Mutation of a highly conserved aspartic acid in the β2 adrenergic receptor: constitutive activation, structural instability, and conformational rearrangement of transmembrane segment 6. Molecular pharmacology. 1999 Jul 1;56(1):175-84.
[5]. Bhogal N, Blaney FE, Ingley PM, Rees J, Findlay JB. Evidence for the proximity of the extreme N-terminus of the neurokinin-2 (NK2) tachykinin receptor to cys167 in the putative fourth transmembrane helix. Biochemistry. 2004 Mar 23;43(11):3027-38.

Chemical Properties

Cas No. 16599-33-0 SDF
Synonyms 2-Aminoethyl methanethiosulfonate, Methanethiosulfonate Ethylammonium
Canonical SMILES NCCSS(C)(=O)=O.Br
Formula C3H9NO2S2•HBr M.Wt 236.1
Solubility DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 10 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.2355 mL 21.1775 mL 42.3549 mL
5 mM 0.8471 mL 4.2355 mL 8.471 mL
10 mM 0.4235 mL 2.1177 mL 4.2355 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 25 reference(s) in Google Scholar.)

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