Bromophenol Blue (Synonyms: Albutest, NSC 7818) |
Catalog No.GC12740
One-Click Copy Product Info
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widely used in gel loading buffers
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 115-39-9
Sample solution is provided at 25 µL, 10mM.
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IC50: N/A
Bromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. Bromophenol blue can be prepared by slowly adding excess bromine to a hot solution ofphenolsulfonphthaleinin the presence of glacial acetic acid.
In vitro: Previous results showed that the adsorption process of bromophenol blue increased with the increase in the concentration of α-chitin nanoparticles (CNP), contact time and temperature, while the adsorption process decreased with increase in the initial dye concentration and strong acidic pH. The Fourier transform infrared spectroscopy results confirmed that the interaction between Bromophenol blue and CNP involved physical adsorption. The adsorption process obeyed Langmuir isotherm and pseudo second order kinetics more effectively. The isotherm and kinetic models confirmed that CNP could be used as a suitable adsorbent material for the removal of dyestuff, such as Bromophenol blue, from effluents [1].
In vivo: So far, bromophenol blue has not been employd in animal in vivo study.
Clinical trial: N/A
Reference:
[1] Dhananasekaran S,Palanivel R,Pappu S. Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles. J Adv Res.2016 Jan;7(1):113-24.
| Cas No. | 115-39-9 | SDF | |
| Synonyms | Albutest, NSC 7818 | ||
| Chemical Name | 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide | ||
| Canonical SMILES | BrC(C=C(C=C1Br)C(C2=CC=CC=C32)(C(C=C4Br)=CC(Br)=C4O)OS3(=O)=O)=C1O | ||
| Formula | C19H10Br4O5S | M.Wt | 669.96 |
| Solubility | ≥ 33.5mg/mL in EtOH | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4926 mL | 7.4631 mL | 14.9263 mL |
| 5 mM | 298.5 μL | 1.4926 mL | 2.9853 mL |
| 10 mM | 149.3 μL | 746.3 μL | 1.4926 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
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Appearance: A solid
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)