Opitor-0 |
Catalog No.GC79122
One-Click Copy Product Info
|
Opitor-0 is a OPA1 GTPase inhibitor with an IC50 of 3.0 μM.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
In Vitro, Opitor-0 (50 μM; 6-24 h) induces mitochondrial fragmentation in MCF10A, MCF10AT1 and MCF10CA1h breast cells; it selectively reduces mitochondrial respiratory efficiency and ATP-coupled respiration in metastatic MCF10CA1h and MCF10CA1a breast cancer cells[1]. Opitor-0 (1-50 μM; 5 h) induces concentration-dependent mitochondrial fragmentation in mtYFP-expressing MEF cells and HeLa cells[2]. Opitor-0 (50 μM; 20 h) selectively impairs the migratory capacity of metastatic MCF10CA1h and MCF10CA1a breast cancer cells, without affecting non-metastatic MCF10A and MCF10AT1 breast cancer cells[1]. Opitor-0 (50 μM; 24 h) selectively reduces the proliferative capacity of highly metastatic MCF10CA1a breast cancer cells, without affecting non-metastatic MCF10A, MCF10AT1 or moderately metastatic MCF10CA1h breast cancer cells[1]. Opitor-0 (50 μM) does not induce cell death in any tested breast cancer cell lines after 24 h of treatment, but causes a small yet significant increase in the level of cell death in metastatic MCF10CA1h and MCF10CA1a breast cancer cells after 72 h of treatment, without affecting non-metastatic MCF10A and MCF10AT1 breast cancer cells[1]. Opitor-0 potently inhibits the GTPase activity of wild-type mini-rOpa1, with an IC50 of 3.0 μM[2]. Opitor-0 (25-125 μM; 30 min) potently enhances cBID-mediated cytochrome c release from purified mouse liver mitochondria[2]. Opitor-0 (5-100 μM; administered for 5 h after 12 h of preincubation with 10-20 μM ABT-737) exerts a strong synergistic effect with the Bcl-2 inhibitor ABT-737 to induce caspase-dependent apoptosis in MDA-MB-231 triple-negative breast cancer cells[2].
References:
[1]. Diokmetzidou A, et al. Metastatic breast cancer cells are selectively dependent on the mitochondrial cristae-shaping protein OPA1. Cell death & disease. 2025 Jul 21;16(1):539.
[2]. Pellattiero A, et al. Small molecule OPA1 inhibitors amplify cytochrome c release and reverse cancer cells resistance to Bcl-2 inhibitors. Sci Adv. 2025 Jul 4;11(27):eadx4562.
| Cas No. | SDF | ||
| Formula | C30H25N5O2S | M.Wt | 519.62 |
| Solubility | DMSO: 35 mg/mL (67.36 mM; Need ultrasonic) | Storage | Store at -20°C |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9245 mL | 9.6224 mL | 19.2448 mL |
| 5 mM | 384.9 μL | 1.9245 mL | 3.849 mL |
| 10 mM | 192.4 μL | 962.2 μL | 1.9245 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)