p53 Bipartite NLS TFA is a bipartite nuclear localization signal derived from p53. When p53 Bipartite NLS TFA is fused to the N-terminus of p65 and the single-component nuclear localization signal of p65 itself is mutated, it alters the nuclear import pathway of p65. p53 Bipartite NLS TFA renders p65 resistant to TTP -mediated inhibition of NF-κB transcriptional activity and nuclear import [1].
In Vitro, p53 Bipartite NLS TFA confers partial resistance to TTP-mediated inhibition of p65-driven NF-κB-dependent luciferase activity in HEK 293 cells[1].
References:
[1]. Schichl YM, et al. Tristetraprolin impairs NF-kappaB/p65 nuclear translocation. J Biol Chem. 2009 Oct 23;284(43):29571-81.
In Vitro, p53 Bipartite NLS TFA confers partial resistance to TTP-mediated inhibition of p65-driven NF-κB-dependent luciferase activity in HEK 293 cells[1].
References:
[1]. Schichl YM, et al. Tristetraprolin impairs NF-kappaB/p65 nuclear translocation. J Biol Chem. 2009 Oct 23;284(43):29571-81.