QTZ |
Catalog No.GC65250
One-Click Copy Product Info
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QTZ is a bioluminescent substrate used for in vivo imaging.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2883232-39-9
Sample solution is provided at 25 µL, 10mM.
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QTZ is a bioluminescent substrate used for in vivo imaging. QTZ features red-shifted emission and low background characteristics, and as a coelenterazine analogue, QTZ bears a 4-quinolyl substitution at the C8 position. QTZ can be used in studies related to in vivo imaging and tumor immunoimaging[1-2].
References:
[1] Lu K, Wang Y, Wang C, et al. A Bioluminescent Probe for H2S Detection in Tumor Microenvironment. ACS Bio Med Chem Au. 2025 Jan 3;5(1):175-183.
[2] Xiong Y, Zhang Y, Li Z, et al. Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo. J Am Chem Soc. 2022 Aug 10;144(31):14101-14111.
QTZ for In Vivo Immuno-Bioluminescence Imaging[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Dissolve QTZ in an in vivo injection buffer to prepare a stock solution at a suitable concentration (can be dissolved up to ~2mM).
2. Paired Enzyme Preparation: Prepare the amber light-emitting mutant luciferase QLuc, which pairs with QTZ. This can be done by using an adeno-associated virus (AAV) system carrying a QLuc expression gene driven by a liver-specific TBG promoter, or by constructing and expressing/purifying a QLuc fusion protein with a targeting antibody fragment (e.g., anti-Her2 scFv).
3. Animal Model and Labeling: For immuno-bioluminescence imaging (ImmunoBLI), inoculate AkaLuc-labeled tumor cells in a xenograft mouse model. Inject AAV expressing QLuc (or the scFv-QLuc fusion protein) via the tail vein, or administer the purified scFv-QLuc fusion protein into tumor-bearing mice via tail vein injection.
4. Imaging Procedure: After QLuc expression is established in the target tissue (e.g., liver) or the scFv-QLuc fusion protein has circulated and accumulated in vivo (e.g., wait for 6 hours post-protein injection), administer QTZ to the mice via intraperitoneal (i.p.) or intravenous (i.v.) injection (e.g., 0.1μmol).
5. Signal Acquisition: Using a cooled CCD camera or bioluminescence imaging system equipped with appropriate filters (consider using a far-red filter of 600-700nm to reduce background interference), perform in vivo imaging on anesthetized mice to capture the amber light emitted by the QLuc-QTZ reaction (emission peak ~585nm).
Precautions:
(1) The QLuc-QTZ luciferase-luciferin pair emits red-shifted light (peak ~585nm), with over 52% of its total emitted light having wavelengths above 600nm, enhancing tissue penetration and signal-to-noise ratio for deep-tissue in vivo imaging.
(2) A The in vitro intrinsic brightness of the QLuc-QTZ pair is approximately 24% of the NLuc-FRZ pair but over 60 times brighter than the FLuc-D-luciferin pair. In a mouse liver model, its long-lasting luminescence signal (>30 minutes) outperforms other tested small single-domain NLuc mutants (teLuc, LumiLuc).
(3) The solubility of QTZ in water is approximately 62.5-125μM, and its solubility in the in vivo injection buffer can reach ~2mM.
(4) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Xiong Y, Zhang Y, Li Z, et al. Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo. J Am Chem Soc. 2022 Aug 10;144(31):14101-14111.
| Cas No. | 2883232-39-9 | SDF | |
| Formula | C28H20N4O | M.Wt | 428.48 |
| Solubility | DMSO : ≥ 25 mg/mL (58.35 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3338 mL | 11.6692 mL | 23.3383 mL |
| 5 mM | 466.8 μL | 2.3338 mL | 4.6677 mL |
| 10 mM | 233.4 μL | 1.1669 mL | 2.3338 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >96.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)