Sedanolide |
Catalog No.GC15216
One-Click Copy Product Info
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Sedanolide, one type of phthalein, is a potent hMAO-B inhibitor with an IC50 value of 0.124±0.033µM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6415-59-4
Sample solution is provided at 25 µL, 10mM.
Sedanolide, one type of phthalein, is a potent hMAO-B inhibitor with an IC50 value of 0.124±0.033µM [1]. Sedanolide has COX-I and COX-II inhibitory activities and topoisomerase-I and topoisomerase-II inhibitory activities[2]. Sedanolide has been widely used in a variety of cancer cell models to inhibit cell growth and has inhibitory effects on a variety of bacteria and fungi[3].
In vitro, Sedanolide treatment at 500μM for 24 hours induced autophagy in J5 cells, which inhibited the PI3K/Akt/mTOR pathway[4]. Treatment with 500μM Sedanolide for 24h resulted in a significant increase in DNA strand breaks in HepG2 cells[5]. Treatment with 100μM Sedanolide for 24h protected luciferase-expressing HepG2 cells from H2O2-induced cell death and reduced intracellular ROS levels[6].
In vivo, Sedanolide treatment via daily administration at a dose of 20mg/kg/day for 14 consecutive days protected mice from dextran sodium sulfate (DSS)-induced colitis, alleviated intestinal inflammation, and enhanced intestinal barrier function[7]. Oral administration of Sedanolide (10mg/kg/day) for 14 consecutive days can activate the Nrf2 pathway and alleviate liver damage in mice treated with Acetaminophen (APAP)[8].
References:
[1] Fan Y, Wang J, Jian J, et al. High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation[J]. Acta Pharmaceutica Sinica B, 2024, 14(4): 1772-1786.
[2] Momin R A, Nair M G. Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds[J]. Phytomedicine, 2002, 9(4): 312-318.
[3] Xie Q, Zhang X, Zhao S, et al. Sedanolide: A review on its chemical compounds, mechanisms and functions[J]. Fitoterapia, 2025: 106732.
[4] Hsieh S L, Chen C T, Wang J J, et al. Sedanolide induces autophagy through the PI3K, p53 and NF-κB signaling pathways in human liver cancer cells[J]. International journal of oncology, 2015, 47(6): 2240-2246.
[5] Woods J A, Jewell C, O'Brien N M. Sedanolide, a natural phthalide from celery seed oil: effect on hydrogen peroxide and tert-butyl hydroperoxide-induced toxicity in HepG2 and CaCo-2 human cell lines[J]. In Vitro & Molecular Toxicology: A Journal of Basic and Applied Research, 2001, 14(3): 233-240.
[6] Tabei Y, Abe H, Suzuki S, et al. Sedanolide activates KEAP1–NRF2 pathway and ameliorates hydrogen peroxide-induced apoptotic cell death[J]. International journal of molecular sciences, 2023, 24(22): 16532.
[7] Li S, Zhuge A, Chen H, et al. Sedanolide alleviates DSS-induced colitis by modulating the intestinal FXR-SMPD3 pathway in mice[J]. Journal of Advanced Research, 2025, 69: 413-426.
[8] Li S, Zhuge A, Xia J, et al. Bifidobacterium longum R0175 protects mice against APAP-induced liver injury by modulating the Nrf2 pathway[J]. Free Radical Biology and Medicine, 2023, 203: 11-23.
| Cell experiment [1]: | |
Cell lines | Luciferase-expressing HepG2 cells |
Preparation Method | Luciferase-expressing HepG2 cells were cultured at 37°C and 5% CO2 in a humidified environment in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 1% minimal essential medium nonessential amino acids (MEM), and 1mM sodium pyruvate. HepG2 cells expressing luciferase were seeded in 12-well plates at a density of 3.6×105 cells/well. After one day of incubation, cells were pretreated with 100µM Sedanolide for 24h and then exposed to hydrogen peroxide for an additional 24h before analysis of hydrogen peroxide-induced cell death. |
Reaction Conditions | 100µM; 24h |
Applications | Sedanolide pretreatment significantly inhibited hydrogen peroxide-induced cell death in Luciferase-expressing HepG2 cells. |
| Animal experiment [2]: | |
Animal models | Male C57BL/6J mice |
Preparation Method | Six-week-old male C57BL/6J mice were raised in an SPF environment and provided with adequate water and food. The mice were exposed to a 12-hour light/12-hour dark cycle and maintained at a temperature of 23±2°C. They were randomly divided into the NC group and the Sedanolide group. The mice were orally gavaged with Sedanolide (20mg/kg) or the vehicle every day for 14 consecutive days, starting from the 7th day to the 14th day. In the Sedanolide group, 2% DSS was given to the mice from the 7th day to the 14th day. On the 15th day, water was substituted for 2% DSS, and the mice were anesthetized and sacrificed on the 16th day. The colon tissues were collected for analysis. |
Dosage form | 20mg/kg/day for 14 days; p.o. |
Applications | Sedanolide treatment improved colitis symptoms, inflammation levels, and intestinal barrier function in mice. |
References: | |
| Cas No. | 6415-59-4 | SDF | |
| Chemical Name | 3-butyl-3a,4,5,6-tetrahydro-1(3H)-isobenzofuranone | ||
| Canonical SMILES | O=C1OC(CCCC)C2CCCC=C21 | ||
| Formula | C12H18O2 | M.Wt | 194.3 |
| Solubility | ≥ 6.65mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.1467 mL | 25.7334 mL | 51.4668 mL |
| 5 mM | 1.0293 mL | 5.1467 mL | 10.2934 mL |
| 10 mM | 514.7 μL | 2.5733 mL | 5.1467 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)