HS-1371 is a potent and ATP-competitive receptor-interacting protein kinase 3 (RIP3) inhibitor with an IC50 of 20.8 nM[1].
HS-1371 directly binds to RIP3 in an ATP-competitive and time-independent manner. HS-1371 has an inhibitory effect on RIP3 kinase activity in various cell lines[1].HS-1371 (0.1, 1, 5, 10, 20 μM, 9 h) can block RIP3 S227 phosphorylation[1].HS-1371 (0.1, 1, 5, 10 μM, 24 h) rescues cells from RIP3-dependent necroptotic cell death but not apoptotic cell death[1].|| Western Blot Analysis[1]||Cell Line:|HT-29 cells |Concentration:|0.1, 1, 5, 10, 20 μM|Incubation Time:|9 h|Result:|HS-1371 shows an inhibitory effect on S227 auto-phosphorylation of RIP3.
[1]. Park HH, et al. HS-1371, a novel kinase inhibitor of RIP3-mediated necroptosis. Exp Mol Med. 2018 Sep 20;50(9):125.