Spermine (Synonyms: Gerontine,Musculamine,Neuridine,NSC 268508,1,5,10,14-Tetraazatetradecane) |
Catalog No.GC14953
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Mixed NMDA glutamate receptor agonist/antagonist
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Cas No.: 71-44-3
Sample solution is provided at 25 µL, 10mM.
Spermine is an endogenous polyamine involved in cellular metabolism found in all eukaryotic cells. Spermine is required for eukaryotic cell growth and protein synthesis. Spermine functions as physiological blockers of inward rectifier K+ channels [1].
Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Inward rectifier K+ channels play an important role in controlling excitability [1].
Spermine blocked cloned inward rectifier K+ channels IRK1 with the IC50 of 31 nM clamped at 50 mV membrane potential [1]. Physiological concentrations of free spermine (10 μM) mediated strong rectification of IRK1 inward rectifier K+ channels even in the absence of free Mg2+ and in IRK1 mutant channels that have no endogenous rectification [1].
High dose level of spermine induced a great number of changes, such as emaciation, aggressiveness, convulsions and paralysis of the hind legs in Wistar rats. Spermine considerably decreased growth, food intake and water intake [3].
References:
[1] Fakler B, Brndle U, Glowatzki E, et al. Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine[J]. Cell, 1995, 80(1): 149-154.
[2] Til H P, Falke H E, Prinsen M K, et al. Acute and subacute toxicity of tyramine, spermidine, spermine, putrescine and cadaverine in rats[J]. Food and Chemical Toxicology, 1997, 35(3): 337-348.
| Cas No. | 71-44-3 | SDF | |
| Synonyms | Gerontine,Musculamine,Neuridine,NSC 268508,1,5,10,14-Tetraazatetradecane | ||
| Chemical Name | N1,N4-bis(3-aminopropyl)-1,4-butanediamine | ||
| Canonical SMILES | NCCCNCCCCNCCCN | ||
| Formula | C10H26N4 | M.Wt | 202.3 |
| Solubility | ≤50mg/ml in Water | Storage | 4°C, away from moisture and light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.9432 mL | 24.7158 mL | 49.4315 mL |
| 5 mM | 988.6 μL | 4.9432 mL | 9.8863 mL |
| 10 mM | 494.3 μL | 2.4716 mL | 4.9432 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50% Appearance: <29°C Solid,>29°C Liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)