Stains-All |
Catalog No.GC30230
One-Click Copy Product Info
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Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 7423-31-6
Sample solution is provided at 25 µL, 10mM.
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Stains-All is a cationic carbocyanine dye.
Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue[1].
[1]. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983 Sep 25;258(18):11267-73.
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Kinase experiment: |
Slab gels are fixed overnight with 25% isopropyl alcohol and washed exhaustively in 25% isopropyl alcohol to remove SDS. The gels are then stained in the dark for at least 48 h with 0.0025% Stains-all, 25% isopropyl alcohol, 7.5% formamide, and 30 mM Tris base, pH 8.8. The interaction of Stains-all with various Ca2+-binding proteins is also studied in aqueous solution. The standard solution contains 10 mM Tris base, pH 8.8, 0.001% Stains-all, and 0.1% formamide. Ca2+-binding proteins (0.5 to 12 μg) are added to 1.0 mL of solution and then incubated at room temperature in the dark for 30 min. The absorbance at 600 nm is then measured against a control solution, containing no protein, using a spectrophotometer[1]. |
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References: [1]. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983 Sep 25;258(18):11267-73. |
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| Cas No. | 7423-31-6 | SDF | |
| Canonical SMILES | CC(/C=C1SC2=CC=C3C=CC=CC3=C2N\1CC)=C\C4=[N+](CC)C5=C6C=CC=CC6=CC=C5S4.[Br-] | ||
| Formula | C30H27BrN2S2 | M.Wt | 559.58 |
| Solubility | DMSO : 65 mg/mL (116.16 mM);Water : < 0.1 mg/mL (insoluble) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7871 mL | 8.9353 mL | 17.8705 mL |
| 5 mM | 357.4 μL | 1.7871 mL | 3.5741 mL |
| 10 mM | 178.7 μL | 893.5 μL | 1.7871 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)