Trypsin inhibitor(soybean) (≥4000 BAEE Units/mg)

Trypsin inhibitor(soybean) is a trypsin inhibitor with an IC₅₀ of 19 µM. It can also inhibit chymotrypsin with an IC₅₀ of 14.3µM^[1]. Trypsin inhibitor (soybean) has strong binding force with trypsin and blocks its active site, K_i=1-10⁻⁵nM^[2]. Trypsin inhibitor (soybean) can be added to cell culture medium to terminate trypsin digestion of cells ^[3]. Trypsin inhibitor (soybean) inhibits trypsin at a 1:1 molar ratio.

In vitro, Trypsin inhibitor (soybean) (0-25µM) treated MCF-7 and HepG2 cells for 72 hours, and both inhibited cell proliferation, with IC₅₀ values of 45.7µM and 23.7µM respectively ^[1]. Trypsin inhibitor (soybean) (10μg/mL) treated HEK and A549 cells for 24 hours, which produced significant cytotoxic effects, was more toxic to A549 cells, and increased the activity of reactive oxygen species scavenging enzymes ^[4].

In vivo, Trypsin inhibitor (soybean) (2.0mg/g) was orally fed to KM mice for 5 weeks, which significantly increased pancreatic mitochondrial MDA content and reduced SOD and GSH-Px levels ^[5].

References:
[1] Ye X, Ng T B. A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans[J]. Planta medica, 2009, 75(05): 550-556.
[2] Blow D M, Janin J, Sweet R M. Mode of action of soybean trypsin inhibitor (Kunitz) as a model for specific protein–protein interactions[J]. Nature, 1974, 249(5452): 54-57.
[3] Stevenson N L, Bergen D J M, Lu Y, et al. Giantin is required for intracellular N-terminal processing of type I procollagen[J]. Journal of Cell Biology, 2021, 220(6): e202005166.
[4] Shamsi T N, Parveen R, Fatima S. Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes[J]. Indian Journal of Pharmacology, 2017, 49(2): 155-160.
[5] Gu C, Yang Q, Li S, et al. Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods[J]. Foods, 2023, 12(8): 1691.