CORM 2 |
رقم الكتالوجGC50347
One-Click Copy Product Info
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CORM 2 هو مانح دوائي لإطلاق ثاني أكسيد الكربون.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 22594-69-0
Sample solution is provided at 25 µL, 10mM.
CORM 2 is a carbon monoxide-releasing molecule that can liberate a controlled amount of CO in biological systems and has been developed as a carbon monoxide donor[1-2]. When CORM 2 is in contact with reduced deoxymyoglobin or deoxyhaemoglobin rapidly transfers ca. one CO equivalent to the protein haem, forming carboxymyoglobin (COMb) or carboxyhaemoglobin (COHb), respectively[3]. In addition, CORM 2 also exerts a protective effect against gastric mucosal injury induced by 75% ethanol[4].
In vitro, after treating human umbilical vein endothelial cells (HUVECs) with 100µM CORM 2 for 0-5min, a bidirectional current can be recorded. This current is insensitive to the small-, intermediate-, and large-conductance Ca2+-activated K+ channel blockers apamin (the small-conductance Ca2+-activated K+ channel blocker), TRAM-34 (the intermediate-conductance Ca2+-activated K+ channel blocker), and iberiotoxin (the big-conductance Ca2+-activated K+ channel blocker), and it is not affected by EGTA in the pipette solution[2]. RAW264.7 murine macrophage cell line was exposed to P. intermedia lipopolysaccharide (LPS) (10μg/mL) together with escalating concentrations of CORM 2 (12.5, 25, 50, 100μM) for 24h (NO) or 48h (IL-1β), and the release of both NO and IL-1β was markedly and dose-dependently suppressed[5].
In vivo, intravenous infusion of CORM 2 at 10mg/kg/h for 30min (total 5mg/kg) after cardiac arrest significantly increased neurological deficit scores (NDS), reduced S-100B levels, and improved 3-day survival in resuscitated Sprague-Dawley rats[6]. Administering CORM 2 (30mg/kg; i.p.) before a lethal dose of LPS significantly attenuated the severe hypothermia induced by LPS in C57BL/6 mice[7].
References:
[1] Khir NAM, Noh ASM, Long I, et al. Recent progress on anti-nociceptive effects of carbon monoxide releasing molecule-2 (CORM-2). Mol Cell Biochem. 2024;479(3):539-552.
[2] Dong DL, Chen C, Huang W, et al. Tricarbonyldichlororuthenium (II) dimer (CORM2) activates non-selective cation current in human endothelial cells independently of carbon monoxide releasing. Eur J Pharmacol. 2008;590(1-3):99-104.
[3] Fagone P, Mangano K, Coco M, et al. Therapeutic potential of carbon monoxide in multiple sclerosis. Clin Exp Immunol. 2012;167(2):179-187.
[4] Magierowska K, Magierowski M, Hubalewska-Mazgaj M, et al. Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage. PLoS One. 2015;10(10):e0140493.
[5] Choi EY, Keum BR, Choe SH, et al. Tricarbonyldichlororuthenium(II) dimer, the lipid-soluble carbon monoxide-releasing molecule, attenuates Prevotella intermedia lipopolysaccharide-induced production of nitric oxide and interleukin-1β in murine macrophages. Int Immunopharmacol. 2021;90:107190.
[6] Wang P, Yao L, Zhou LL, et al. Carbon Monoxide Improves Neurologic Outcomes by Mitochondrial Biogenesis after Global Cerebral Ischemia Induced by Cardiac Arrest in Rats. Int J Biol Sci. 2016;12(8):1000-1009.
[7] Riquelme SA, Bueno SM, Kalergis AM. Carbon monoxide down-modulates Toll-like receptor 4/MD2 expression on innate immune cells and reduces endotoxic shock susceptibility. Immunology. 2015;144(2):321-332
| Cell experiment [1]: | |
Cell lines | RAW264.7 murine macrophage cell line |
Preparation Method | Cells were incubated with the increasing dosages of CORM 2 (12.5-100μM) for 24h (measure NO) or 48h (measure IL-1β) in the presence of P. intermedia lipopolysaccharide (LPS) (10μg/ml). |
Reaction Conditions | 12.5, 25, 50, 100μM; 24h (measure NO) or 48h (measure IL-1β) |
Applications | The secretion levels of NO and IL-1β caused by P. intermedia LPS alone were obviously upregulated over those of the untreated control cells, and the administration of CORM 2 potently reduced the release of these proinflammatory mediators, and the suppressive influence was elevated with the increase of doses. CORM 2 reduced the synthesis of NO and IL-1β by approximately 83% and 84%, respectively, at the concentration of 100μM. |
| Animal experiment [2]: | |
Animal models | Male Sprague-Dawley rats |
Preparation Method | Cardiac arrest was induced by asphyxia. After 6min of untreated arrest, cardiopulmonary resuscitation (CPR) was initiated. Return of spontaneous circulation (ROSC) was defined as the restoration of a supraventricular rhythm with a mean arterial pressure (MAP)≥60mmHg sustained for at least 5min. After successful resuscitation, animals were randomly assigned to three groups: (1) CPR group—cardiac arrest was induced and CPR performed; (2) CPR+CO group—cardiac arrest was induced and CPR performed, followed by treatment with CORM 2; (3) Control group—identical surgical procedures were carried out, but cardiac arrest was not induced and CPR was omitted. Thirty minutes after ROSC, rats in the CPR+CO group received a continuous intravenous infusion of CORM 2 at 10mg/kg/h for 30min (total dose 5mg/kg). Concurrently, rats in the control and CPR groups received an equal volume of placebo (0.9% NaCl solution containing DMSO) at an infusion rate of 4mL/kg/h. |
Dosage form | 5mg/kg; intravenous infusion |
Applications | The CORM 2 treatment results showed that CO increased Neurologic deficit scores (NDS) and decreased S-100B levels significantly at 24h after ROSC. CO treatment significantly increased the 3-day survival rates of the rats after ROSC. |
References: | |
| Cas No. | 22594-69-0 | SDF | |
| Canonical SMILES | [Cl-][Ru++]1([Cl-][Ru++]([Cl-])([Cl-]1)(C#[O])(C#[O])C#[O])(C#[O])(C#[O])C#[O] | ||
| Formula | C6Cl4O6Ru2 | M.Wt | 512.01 |
| الذوبان | DMSO : 30 mg/mL (58.59 mM; Need ultrasonic); H2O : < 0.1 mg/mL (insoluble) | Storage | 4°C, away from moisture and light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9531 mL | 9.7654 mL | 19.5309 mL |
| 5 mM | 390.6 μL | 1.9531 mL | 3.9062 mL |
| 10 mM | 195.3 μL | 976.5 μL | 1.9531 mL |
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- Purity: >98.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)