Polyphyllin VI |
رقم الكتالوجGN10249
One-Click Copy Product Info
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Polyphyllin VI is an orally active natural saponin that exerts anticancer effects primarily by inducing cell-cycle arrest, apoptosis, and pyroptosis.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 55916-51-3
Sample solution is provided at 25 µL, 10mM.
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Polyphyllin VI is an orally active natural saponin that exerts anticancer effects primarily by inducing cell-cycle arrest, apoptosis, and pyroptosis[1][2]. Polyphyllin VI is commonly used in cancer-mechanism studies, especially those focused on cell-death pathways and their associated signaling cascades[3].
In vitro, treatment of glioma cells with Polyphyllin VI (0-8μM; 12-48h) dose-dependently inhibits cell proliferation, blocks cycle at G2/M, suppresses migration, and induces apoptosis and autophagy[4]. Polyphyllin VI (0-30μM; 48h) inhibits U2OS osteosarcoma proliferation, triggers G2/M arrest, collapses mitochondrial membrane potential, and simultaneously evokes caspase-mediated apoptosis[5].
In vivo, Polyphyllin VI (5mg/kg/day; i.p.; 14 days) reversed bleomycin-induced pulmonary fibrosis in C57BL/6J mice, reduced collagen deposition, suppressed α-SMA and vimentin expression, and restored E-cadherin levels[6]. Polyphyllin VI (10mg/kg/day; i.p.; 10 days) increased NLRP3 inflammasome activation and caspase-1-mediated pyroptosis in A549 tumor xenografts of athymic nude mice[7].
References:
[1] Lin Z, Liu Y, Li F, et al. Anti-lung Cancer Effects of Polyphyllin VI and VII Potentially Correlate with Apoptosis In Vitro and In Vivo. Phytother Res. 2015;29(10):1568-1576.
[2] Liu Y, Dong X, Wang W, et al. Molecular Mechanisms of Apoptosis in HepaRG Cell Line Induced by Polyphyllin VI via the Fas Death Pathway and Mitochondrial-Dependent Pathway. Toxins (Basel). 2018;10(5):201.
[3] Wang Y, Huang X, Xian B, et al. Machine learning and bioinformatics-based insights into the potential targets of saponins in Paris polyphylla smith against non-small cell lung cancer. Front Genet. 2022;13:1005896.
[4] Liu W, Chai Y, Hu L, et al. Polyphyllin VI Induces Apoptosis and Autophagy via Reactive Oxygen Species Mediated JNK and P38 Activation in Glioma. Onco Targets Ther. 2020;13:2275-2288.
[5] Yuan YL, Jiang N, Li ZY, et al. Polyphyllin VI induces apoptosis and autophagy in human osteosarcoma cells by modulation of ROS/JNK activation. Drug Des Devel Ther. 2019;13:3091-3103.
[6] Xie Y, Gan C, Liu H, et al. Polyphyllin VI Ameliorates Pulmonary Fibrosis by Suppressing the MAPK/ERK and PI3K/AKT Signaling Pathways via Upregulating DUSP6. Phytother Res. 2024;38(12):5930-5948.
[7] Teng JF, Mei QB, Zhou XG, et al. Polyphyllin VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD Signal Axis in Non-Small Cell Lung Cancer. Cancers (Basel). 2020;12(1):193.
| Cell experiment [1]: | |
Cell lines | Glioma Cell lines U87, LN229, U251, and U343 |
Preparation Method | Glioma Cell lines U87, LN229, U251, and U343 were cultured in DMEM medium supplemented with 10% FBS and maintained in an incubator at 37°C, and 5% CO2. Approximately 2000 glioma cells were re-suspended in 100µL of complete medium and cultured in 6-well plates for 24 hrs. The cells were treated with varying concentrations of Polyphyllin VI (0, 2, 4, 6, and 8µM) followed by a 12, 24 and 48 hrs incubation. Thereafter, the cells were treated with the WST-8 reagent from CCK-8 for 2 hrs, then their viability assayed by detecting the absorbance at OD 450nm. Cell apoptosis was analyzed using the Annexin V-FITC Kit. Cell cycle distribution was detected by flow cytometry. Transwell assay was carried out to determine the role of Polyphyllin VI on the invasive ability of glioma cells. |
Reaction Conditions | 0-8μM; 12-48h |
Applications | Polyphyllin VI (0-8μM; 12-48h) dose-dependently inhibits cell proliferation, suppresses migration, and induces apoptosis in glioma cells. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | C57BL/6 mice were first anesthetized with 5mg/mL pentobarbital sodium. Then, 100μL of normal saline containing BLM sulfate(2mg/kg) was directly injected into the trachea of C57BL/6J mice. For the therapeutic experiment, the mice were randomly categorized into two groups on the seventh day following BLM modeling: the BLM+solvent (dimethyl sulfoxide: polyethylene glycol 400: normal saline=5:45:50) group and the BLM+Polyphyllin VI (5mg/kg/day) group. All mice were injected intraperitoneally with PPVI for 14 days, during which the mice's weight was measured every 3 days. Then the mice were sacrificed for tissue sample collection, and the weight of the lungs was recorded. Histopathological and immunohistochemical (IHC) tests were conducted on a lung excised from the left and soaked in 4% paraformaldehyde. |
Dosage form | 5mg/kg/day; i.p.; 14 days |
Applications | Polyphyllin VI reversed bleomycin-induced pulmonary fibrosis in C57BL/6J mice, reduced collagen deposition, suppressed α-SMA and vimentin expression, and restored E-cadherin levels. |
References: | |
| Cas No. | 55916-51-3 | SDF | |
| Chemical Name | Chonglou saponin VI | ||
| Canonical SMILES | O[C@@H]([C@@H](O)[C@H]1O)[C@]([H])(O[C@H]2[C@@](O[C@H]3CC4=CC[C@]([C@](C[C@@](O[C@]5(OC6)CC[C@H]6C)([H])[C@]7(O)[C@@H]5C)([H])[C@@]7(CC8)C)([H])[C@@]8([H])[C@]4(CC3)C)([H])O[C@H](CO)[C@@H](O)[C@@H]2O)C[C@@H]1C | ||
| Formula | C39H62O13 | M.Wt | 738.91 |
| الذوبان | ≥ 73.9mg/mL in EtOH with gentle warming | Storage | Store at -20℃ |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3533 mL | 6.7667 mL | 13.5334 mL |
| 5 mM | 270.7 μL | 1.3533 mL | 2.7067 mL |
| 10 mM | 135.3 μL | 676.7 μL | 1.3533 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)