Polyphyllin VI

Cell lines

Glioma Cell lines U87, LN229, U251, and U343

Preparation Method

Glioma Cell lines U87, LN229, U251, and U343 were cultured in DMEM medium supplemented with 10% FBS and maintained in an incubator at 37°C, and 5% CO2. Approximately 2000 glioma cells were re-suspended in 100µL of complete medium and cultured in 6-well plates for 24 hrs. The cells were treated with varying concentrations of Polyphyllin VI (0, 2, 4, 6, and 8µM) followed by a 12, 24 and 48 hrs incubation. Thereafter, the cells were treated with the WST-8 reagent from CCK-8 for 2 hrs, then their viability assayed by detecting the absorbance at OD 450nm. Cell apoptosis was analyzed using the Annexin V-FITC Kit. Cell cycle distribution was detected by flow cytometry. Transwell assay was carried out to determine the role of Polyphyllin VI on the invasive ability of glioma cells.

Reaction Conditions

0-8μM; 12-48h

Applications

Polyphyllin VI (0-8μM; 12-48h) dose-dependently inhibits cell proliferation, suppresses migration, and induces apoptosis in glioma cells.

Animal models

C57BL/6 mice

Preparation Method

C57BL/6 mice were first anesthetized with 5mg/mL pentobarbital sodium. Then, 100μL of normal saline containing BLM sulfate(2mg/kg) was directly injected into the trachea of C57BL/6J mice. For the therapeutic experiment, the mice were randomly categorized into two groups on the seventh day following BLM modeling: the BLM+solvent (dimethyl sulfoxide: polyethylene glycol 400: normal saline=5:45:50) group and the BLM+Polyphyllin VI (5mg/kg/day) group. All mice were injected intraperitoneally with PPVI for 14 days, during which the mice's weight was measured every 3 days. Then the mice were sacrificed for tissue sample collection, and the weight of the lungs was recorded. Histopathological and immunohistochemical (IHC) tests were conducted on a lung excised from the left and soaked in 4% paraformaldehyde.

Dosage form

5mg/kg/day; i.p.; 14 days

Applications

Polyphyllin VI reversed bleomycin-induced pulmonary fibrosis in C57BL/6J mice, reduced collagen deposition, suppressed α-SMA and vimentin expression, and restored E-cadherin levels.

References:
[1] Liu W, Chai Y, Hu L, et al. Polyphyllin VI Induces Apoptosis and Autophagy via Reactive Oxygen Species Mediated JNK and P38 Activation in Glioma. Onco Targets Ther. 2020;13:2275-2288.
[2] Xie Y, Gan C, Liu H, et al. Polyphyllin VI Ameliorates Pulmonary Fibrosis by Suppressing the MAPK/ERK and PI3K/AKT Signaling Pathways via Upregulating DUSP6. Phytother Res. 2024;38(12):5930-5948.