Chktide acetate |
Catalog No.GC79076
One-Click Copy Product Info
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Chktide acetate is a synthetic peptide substrate applicable for activity assays of Chk1 kinase and cyk/CaMKII.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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In Vitro, Chktide acetate serves as a standard synthetic substrate for Chk1 serine protein kinase assays and can be used for the quantitative detection of Chk1 catalytic activity[1]. Chktide acetate contains the Ser53 phosphorylation site of Xenopus cyclin B2, and upon phosphorylation, it induces a prophase-like premature phenotype in fibroblasts[2].
References:
[1]. Walker M, et al. Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity. Oncogene. 2009 Jun 18;28(24):2314-23.
[2]. Stevens I, et al. Identification of cyk, a cyclin B2 kinase, as a novel calcium/calmodulin-dependent protein kinase II and its role during Xenopus laevis oocyte maturation. Experimental cell research. 1999 Nov 01;252(2):303-18.
| Cas No. | SDF | ||
| Formula | C115H198N40O33S·xC2H4O2 | M.Wt | 2701.12 |
| Solubility | H2O: 50 mg/mL (Need ultrasonic) | Storage | Sealed storage, away from moisture |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 370.2 μL | 1.8511 mL | 3.7022 mL |
| 5 mM | 74 μL | 370.2 μL | 740.4 μL |
| 10 mM | 37 μL | 185.1 μL | 370.2 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)