GLPBio

CMK

Catalog No.: GC14693Purity:>99.50%
COASDSData Sheet

Products are for research use only. Not for human use. We do not sell to patients.

CMK
Cas No.: 821794-90-5
10mgIn Stock
$186.00
1
25mgIn Stock
$259.00
1
100mgIn Stock
$603.00
1
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Citations

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    Nature
    641, 529–536 (2025)
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    388(6745) (2025)
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    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 273–287 (2023)
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    33, 546–561 (2023)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

Description

CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK.

CMK inhibits the growth of Cdc5 (L158G) with IC50 of 36 nM, greater than 30 μM for wild type Cdc5. CMK exhibits a concentration-dependent first cell cycle mitotic arrest in the cdc5-as1 strain with an IC50 of 1.1 μM. CMK inhibition of Cdc5 (L158G) leads to a first cell cycle anaphase arrest and delay in anaphase spindle migration[1].

References:
[1]. Snead JL, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007 Nov;14(11):1261-72.

Protocol

Kinase experiment:

For Cdc5 kinase assays, Spc72-TAP is isolated from cell extract by pulldown with IgG sepharose or rabbit IgG coupled to M-270 epoxy dynabeads and incubated in kinase buffer (25 mM HEPES, pH 8.0, 60 mM KCl, 15 mM MnCl2, 100 μg/mL BSA, 80 nM microcystin, 1mM DTT, 100 μM 200 μCi/mL [γ-32P]ATP) in the absence or presence of 100 ng purified baculovirus expressed 6xHis-Cdc5. 32P incorporation is visualized on a Typhoon PhosphorImager, and images are processed using ImageQuant software.

References:

[1]. Snead JL, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007 Nov;14(11):1261-72.

Product Documents

Purity:>99.50%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
821794-90-5
Chemical Name
1-[4-amino-7-(3-hydroxypropyl)-5-(4-methylphenyl)pyrrolo[2,3-d]pyrimidin-6-yl]-2-chloroethanone
SMILES
CC1=CC=C(C=C1)C2=C(N(C3=C2C(=NC=N3)N)CCCO)C(=O)CCl
Molecular Formula
C18H19ClN4O2
Molecular Weight
358.83
Solubility
Soluble in DMSO
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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