Cytidine-d2 is intended for use as an internal standard for the quantification of cytidine by GC- or LC-MS. Cytidine is a pyrimidine nucleoside composed of ribose and a cytosine base.1 It forms complementary base pairs with the purine nucleoside guanosine in DNA and RNA. Cytidine is a precursor of uridine and can also be phosphorylated by uridine-cytidine kinase to form cytidine 5’-monophosphate .2,1 It rescues inhibition of proliferation induced by knockdown of CTP synthase 1 in T cells when used at a concentration of 200 µM.3
1.Berg, J.M., Tymoczko, J.L., and Stryer, L.Nucleotide biosynthesisBiochemistry(2002) 2.Chabner, B.A., Johns, D.G., Coleman, C.N., et al.Purification and properties of cytidine deaminase from normal and leukemic granulocytesJ. Clin. Invest.53(3)922-931(1974) 3.Martin, E., Palmic, N., Sanquer, S., et al.CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferationNature510(7504)288-292(2014)