DPBQ |
Katalog-Nr.GC33384
One-Click Copy Product Info
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DPBQ aktiviert p53 und lÖst polyploid-spezifisch Apoptose aus, hemmt aber weder Topoisomerase noch bindet es DNA. DPBQ lÖst die Expression und Phosphorylierung von p53 aus und diese Wirkung ist spezifisch fÜr tetraploide Zellen.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 7029-89-2
Sample solution is provided at 25 µL, 10mM.
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DPBQ is a p53 activator.
DPBQ elicits apoptosis and cell death and is selective for effects in 4N cells. DPBQ elicits expression and phosphorylation of p53 and this effect is specific to tetraploid cells. Knockdown of TP53 restores proliferation of tetraploid cells in the presence of DPBQ[1].
[1]. Choudhary A, et al. Identification of Selective Lead Compounds for Treatment of High-Ploidy Breast Cancer. Mol Cancer Ther. 2016 Jan;15(1):48-59.
Kinase experiment: | For Western blot analysis, 2N and 4N RPE1 cells are treated with DMSO, 50 ng/mL doxorubicin, or 1 μM DPBQ for 48 hours. Western blotting is performed with a SDS-PAGE Electrophoresis and semidry transfer. Samples are analyzed by chemiluminescence. β-actin is used as a protein loading control[1]. |
Cell experiment: | For Apoptosis assays, 2N and 4N RPE1 cells are treated with DMSO, 60 nM staurosporine, or 1 μM DPBQ for 48 hours. Apoptosis is evaluated by an Annexin V-PE/7-AAD apoptosis detection kit. For the flow cytometry analysis, the percent positive cells in the upper right and lower right quadrants are summed to give the total number of apoptotic cells. Three independent replicates are performed[1]. |
References: [1]. Choudhary A, et al. Identification of Selective Lead Compounds for Treatment of High-Ploidy Breast Cancer. Mol Cancer Ther. 2016 Jan;15(1):48-59. | |
| Cas No. | 7029-89-2 | SDF | |
| Canonical SMILES | O=C(C1=CC=CC=C12)C3=C(N=C(C4=CC=CC=C4)C(C5=CC=CC=C5)=N3)C2=O | ||
| Formula | C24H14N2O2 | M.Wt | 362.38 |
| Löslichkeit | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7595 mL | 13.7977 mL | 27.5953 mL |
| 5 mM | 551.9 μL | 2.7595 mL | 5.5191 mL |
| 10 mM | 276 μL | 1.3798 mL | 2.7595 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)