Patulin
Catalog No.: GC40483Purity:>98.00%Synonyms: Clairformin, Expansin, Mycoin C, NSC 8120, NSC 32951
Patulin (Terinin) ist ein Mykotoxin, das von Pilzen einschließlich der Arten Aspergillus, Penicillium und Byssochlamys produziert wird und im Verdacht steht, klastogen, mutagen, teratogen und zytotoxisch zu sein.
Die Produkte sind ausschließlich für Forschungszwecke bestimmt. Nicht zur Anwendung am Menschen. Wir verkaufen nicht an Patienten.
Cas No.: 149-29-1
5mgIn Stock
$97.00
1
10mgIn Stock
$183.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 5mg | $97.00 | In Stock | 1 | |
| 10mg | $183.00 | In Stock | 1 |
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Zitate (Citations)
1 publicationsPublikationen von Forschern, die dieses Produkt verwendenBeschreibung (Description)
Patulin is a toxic polyphenolic lactolactone [1]. Patulin reacts with protein sulfhydryl groups, triggering cellular oxidative stress and p53-mediated DNA damage, which damages the intestinal epithelial barrier [2]. Patulin has teratogenic, genotoxic, and neurotoxic properties [3-4].
In HL-60 cells, after Patulin (0-10μM; 6h) significantly decreased cell viability [5]. In HepG2 cells, Patulin (0.5-2μM; 24h) induces autophagy in cells via the ROS-Akt1-MTOR pathway [6].
In CF-1 mice, Patulin (1.0-3.75mg/kg; ip; single injection) induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney [7]. In B16F10 cell xenograft mouse model, Patulin (5-50μg/kg; ip; 20d) significantly induced tumor regression [8].
References:
[1]. Puel O, Galtier P, Oswald I P. Biosynthesis and toxicological effects of patulin[J]. Toxins, 2010, 2(4): 613-631.
[2]. Saxena N, Ansari K M, Kumar R, et al. Patulin causes DNA damage leading to cell cycle arrest and apoptosis through modulation of Bax, p53 and p21/WAF1 proteins in skin of mice[J]. Toxicology and applied pharmacology, 2009, 234(2): 192-201.
[3]. Glaser N, Stopper H. Patulin: Mechanism of genotoxicity[J]. Food and Chemical Toxicology, 2012, 50(5): 1796-1801.
[4]. Lei W L, Li Y Y, Hou Y, et al. Toxic effects of patulin on mouse oocytes and its possible mechanisms[J]. Toxicology, 2021, 464: 153013.
[5]. Wu T S, Liao Y C, Yu F Y, et al. Mechanism of patulin-induced apoptosis in human leukemia cells (HL-60)[J]. Toxicology letters, 2008, 183(1-3): 105-111.
[6]. Yang G, Bai Y, Wu X, et al. Patulin induced ROS-dependent autophagic cell death in Human Hepatoma G2 cells[J]. Chemico-Biological Interactions, 2018, 288: 24-31.
[7]. de Melo F T, de Oliveira I M, Greggio S, et al. DNA damage in organs of mice treated acutely with patulin, a known mycotoxin[J]. Food and Chemical Toxicology, 2012, 50(10): 3548-3555.
[8]. Boussabbeh M, Ben Salem I, Rjiba-Touati K, et al. The potential effect of patulin on mice bearing melanoma cells: an anti-tumour or carcinogenic effect?[J]. Tumor Biology, 2016, 37(5): 6285-6295.
Protokoll (Protocol)
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 (1 × 105 cells) were seeded in 96-well plates, treated with vehicle (15% ethanol in 0.01M PBS) alone or various concentrations (final concentration 0-10μM) of Patulin at the designated time. |
Reaction Conditions | 0-10μM; 6h |
Applications | Patulin treatment significantly decreased cell viability. |
| Animal experiment [2]: | |
Animal models | CF-1 mice |
Preparation Method | The experimental design involved nine groups of 10 mice, housed individually in cages. Mice received a single intraperitoneal (i.p.) dose of 1.0, 2.5, or 3.75mg/kg body weight (BW) of Patulin. Patulin stock solution was dissolved in DMSO and then diluted with saline to a final DMSO concentration below 0.1%. Mice were divided into the following groups: Group 1: Control group, administered with 0.1% DMSO in saline (5mL/kg BW). Group 2: Positive control group, administered with MMS (40mg/kg BW). Groups 3-5: Patulin was administered intraperitoneally at the following doses: Group 3, 1.0mg/kg BW; Group 4, 2.5mg/kg BW; Group 5, 3.75mg/kg BW. Group 6: N-ac (1840μmol/kg BW) was administered 3 hours prior to administration of 0.1% DMSO (5mL/kg BW). Groups 7-9 were intraperitoneally injected with Patulin at the following doses: 1.0mg/kg body weight for Group 7; 2.5mg/kg body weight for Group 8; and 3.75mg/kg body weight for Group 9. All animals in Groups 7-9 were given 300mg/kg of N-ac 3 hours before Patulin. |
Dosage form | 1.0-3.75mg/kg; ip; single injection |
Applications | Patulin induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney. |
References: | |
Produktdokumente (Product Documents)
Purity:>98.00%Appearance:A solid
Chemische Eigenschaften (Chemical Properties)
CAS-Nr.
149-29-1
Synonyme
Clairformin, Expansin, Mycoin C, NSC 8120, NSC 32951
Chemischer Name
4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one
SMILES
O=C1OC2=CCOC(O)C2=C1
Summenformel
C7H6O4
Molekulargewicht
154.1
Löslichkeit
13mg/mL in ethanol, 10mg/mL in DMSO, 20mg/mL in DMF
Lagerung
-20°C, protect from light
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.
Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit
g/mol
Reviews
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