GLPBio

Ecdysterone

Catalog No.: GN10564Purity:>99.50%Synonyms: Ecdysterone, Isoinokosterone
COASDSData Sheet
Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods.

Products are for research use only. Not for human use. We do not sell to patients.

Ecdysterone
Cas No.: 5289-74-7
10mM (in 1mL DMSO)In Stock
$26.00
1
5mgIn Stock
$25.00
1
10mgIn Stock
$35.00
1
50mgIn Stock
$140.00
1
100mgIn Stock
$224.00
1
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Description

Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods[1]. Ecdysterone can promote protein synthesis in skeletal muscle cells[2]. Ecdysterone can be used as a sports nutrition supplement and in metabolic disease research[3, 4].

In vitro, Ecdysterone (0-500μM) treatment of MCF7, MDA-MB-231, and MDA-MB-468 cells for 96h inhibited colony formation, cell proliferation, and induced cell death in the three cell lines[5]. Ecdysterone (1, 50, 100μM) pretreatment of PC12 cells for 24h reversed the cytotoxic effects induced by β-amyloid peptide fragment 25-35 (Aβ25-35) in a dose-dependent manner[6].

In vivo, subcutaneous injection of Ecdysterone (0.6, 0.8, 1mg/kg) in osteoarthritis (OA) rats for 4 weeks significantly improved the pathological state of cartilage tissue, inhibited inflammation in cartilage tissue, and upregulated the expression levels of autophagy markers LC3II/LC3I in cartilage tissue[7].

References:
[1] Lafont R, Balducci C, Dinan L. Ecdysteroids[J]. Encyclopedia, 2021, 1(4): 1267-1302.
[2] Gorelick-Feldman J, MacLean D, Ilic N, et al. Phytoecdysteroids increase protein synthesis in skeletal muscle cells[J]. Journal of agricultural and food chemistry, 2008, 56(10): 3532-3537.
[3] Isenmann E, Ambrosio G, Joseph J F, et al. Ecdysteroids as non-conventional anabolic agent: performance enhancement by ecdysterone supplementation in humans[J]. Archives of toxicology, 2019, 93(7): 1807-1816.
[4] Todorova V, Ivanova S, Chakarov D, et al. Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition[J]. Nutrients, 2024, 16(9): 1382.
[5] Shuvalov O, Fedorova O, Tananykina E, et al. An arthropod hormone, ecdysterone, inhibits the growth of breast cancer cells via different mechanisms[J]. Frontiers in Pharmacology, 2020, 11: 561537.
[6] Su-fen Y, Zhong-jun W, Zheng-qin Y, et al. Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid 25–35[J]. Chinese journal of integrative medicine, 2005, 11: 293-296.
[7] Tang Y, Mo Y, Xin D, et al. β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway[J]. American Journal of Translational Research, 2020, 12(11): 7174.

Protocol

Cell experiment [1]:

Cell lines

MCF7, MDA-MB-231, MDA-MB-468 cells

Preparation Method

1000 cells were planted per well on a 6-well plate, in triplicates. 24h later, the cells were treated with 0, 100, 150, 250, 350 or 500μM Ecdysterone for 96h. After treatment, fresh media was added, and cells were grown for 10 days. After the indicated time, cells were fixed with acetic acid/methanol (1:7, v/v) and stained with 0.5% crystal violet.

Reaction Conditions

0, 100, 150, 250, 350, 500μM; 96h

Applications

Photographs of plates with colonies clearly showed that not only the number of colonies, but also their size, was significantly reduced upon treatment with Ecdysterone.

Animal experiment [2]:

Animal models

Osteoarthritis (OA) rats

Preparation Method

The OA rats were treated with 30mg/kg 3-methyladenine, 1mg/kg rapamycin, 0.6mg/kg Ecdysterone, 0.8mg/kg Ecdysterone and 1mg/kg Ecdysterone, respectively, with model rats without dosing as control. The administration route for 3-methyladenine and rapamycin was intraperitoneal injection and that for Ecdysterone was subcutaneous injection. The dosing frequency for all the 5 groups was twice a week for 4 weeks.

Dosage form

0.6, 0.8, 1mg/kg; twice a week for 4 weeks; s.c.

Applications

OA was alleviated by Ecdysterone on rats. The expression ratio of LC3II/LC3I was promoted greatly by Ecdysterone.

References:
[1]Shuvalov O, Fedorova O, Tananykina E, et al. An arthropod hormone, ecdysterone, inhibits the growth of breast cancer cells via different mechanisms[J]. Frontiers in Pharmacology, 2020, 11: 561537.
[2]Tang Y, Mo Y, Xin D, et al. β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway[J]. American Journal of Translational Research, 2020, 12(11): 7174.

Product Documents

Purity:>99.50%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
5289-74-7
Synonyms
Ecdysterone, Isoinokosterone
Chemical Name
(2S,3R,5R,9R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-[(2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl]-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one
SMILES
CC12CCC3C(=CC(=O)C4C3(CC(C(C4)O)O)C)C1(CCC2C(C)(C(CCC(C)(C)O)O)O)O
Molecular Formula
C27H44O7
Molecular Weight
486.64
Solubility
≥ 23.25mg/mL in DMSO
Storage
4°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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