Ecdysterone (Synonyms: Ecdysterone, Isoinokosterone) |
Catalog No.GN10564
One-Click Copy Product Info
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Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5289-74-7
Sample solution is provided at 25 µL, 10mM.
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Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods[1]. Ecdysterone can promote protein synthesis in skeletal muscle cells[2]. Ecdysterone can be used as a sports nutrition supplement and in metabolic disease research[3, 4].
In vitro, Ecdysterone (0-500μM) treatment of MCF7, MDA-MB-231, and MDA-MB-468 cells for 96h inhibited colony formation, cell proliferation, and induced cell death in the three cell lines[5]. Ecdysterone (1, 50, 100μM) pretreatment of PC12 cells for 24h reversed the cytotoxic effects induced by β-amyloid peptide fragment 25-35 (Aβ25-35) in a dose-dependent manner[6].
In vivo, subcutaneous injection of Ecdysterone (0.6, 0.8, 1mg/kg) in osteoarthritis (OA) rats for 4 weeks significantly improved the pathological state of cartilage tissue, inhibited inflammation in cartilage tissue, and upregulated the expression levels of autophagy markers LC3II/LC3I in cartilage tissue[7].
References:
[1] Lafont R, Balducci C, Dinan L. Ecdysteroids[J]. Encyclopedia, 2021, 1(4): 1267-1302.
[2] Gorelick-Feldman J, MacLean D, Ilic N, et al. Phytoecdysteroids increase protein synthesis in skeletal muscle cells[J]. Journal of agricultural and food chemistry, 2008, 56(10): 3532-3537.
[3] Isenmann E, Ambrosio G, Joseph J F, et al. Ecdysteroids as non-conventional anabolic agent: performance enhancement by ecdysterone supplementation in humans[J]. Archives of toxicology, 2019, 93(7): 1807-1816.
[4] Todorova V, Ivanova S, Chakarov D, et al. Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition[J]. Nutrients, 2024, 16(9): 1382.
[5] Shuvalov O, Fedorova O, Tananykina E, et al. An arthropod hormone, ecdysterone, inhibits the growth of breast cancer cells via different mechanisms[J]. Frontiers in Pharmacology, 2020, 11: 561537.
[6] Su-fen Y, Zhong-jun W, Zheng-qin Y, et al. Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid 25–35[J]. Chinese journal of integrative medicine, 2005, 11: 293-296.
[7] Tang Y, Mo Y, Xin D, et al. β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway[J]. American Journal of Translational Research, 2020, 12(11): 7174.
| Cell experiment [1]: | |
Cell lines | MCF7, MDA-MB-231, MDA-MB-468 cells |
Preparation Method | 1000 cells were planted per well on a 6-well plate, in triplicates. 24h later, the cells were treated with 0, 100, 150, 250, 350 or 500μM Ecdysterone for 96h. After treatment, fresh media was added, and cells were grown for 10 days. After the indicated time, cells were fixed with acetic acid/methanol (1:7, v/v) and stained with 0.5% crystal violet. |
Reaction Conditions | 0, 100, 150, 250, 350, 500μM; 96h |
Applications | Photographs of plates with colonies clearly showed that not only the number of colonies, but also their size, was significantly reduced upon treatment with Ecdysterone. |
| Animal experiment [2]: | |
Animal models | Osteoarthritis (OA) rats |
Preparation Method | The OA rats were treated with 30mg/kg 3-methyladenine, 1mg/kg rapamycin, 0.6mg/kg Ecdysterone, 0.8mg/kg Ecdysterone and 1mg/kg Ecdysterone, respectively, with model rats without dosing as control. The administration route for 3-methyladenine and rapamycin was intraperitoneal injection and that for Ecdysterone was subcutaneous injection. The dosing frequency for all the 5 groups was twice a week for 4 weeks. |
Dosage form | 0.6, 0.8, 1mg/kg; twice a week for 4 weeks; s.c. |
Applications | OA was alleviated by Ecdysterone on rats. The expression ratio of LC3II/LC3I was promoted greatly by Ecdysterone. |
References: | |
| Cas No. | 5289-74-7 | SDF | |
| Synonyms | Ecdysterone, Isoinokosterone | ||
| Chemical Name | (2S,3R,5R,9R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-[(2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl]-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one | ||
| Canonical SMILES | CC12CCC3C(=CC(=O)C4C3(CC(C(C4)O)O)C)C1(CCC2C(C)(C(CCC(C)(C)O)O)O)O | ||
| Formula | C27H44O7 | M.Wt | 486.64 |
| Solubility | ≥ 23.25mg/mL in DMSO | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0549 mL | 10.2745 mL | 20.5491 mL |
| 5 mM | 411 μL | 2.0549 mL | 4.1098 mL |
| 10 mM | 205.5 μL | 1.0275 mL | 2.0549 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)