GLPBio

Phytic acid dodecasodium salt hydrate

Catalog No.: GC36903Purity:>98.00%
COASDSData Sheet
Phytic acid (Inositol hexaphosphate) dodecasodium hydrate is a phosphorus storage compound of seeds and cereal grains.

Products are for research use only. Not for human use. We do not sell to patients.

Phytic acid dodecasodium salt hydrate
Cas No.: 123408-98-0
10mM (in 1mL Water)In Stock
$52.00
1
250mgIn Stock
$46.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

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Description

Phytic acid dodecasodium salt hydrate is an antinutrient found naturally in legume seeds [1]. Phytic acid dodecasodium salt hydrate and its degradation products can scavenge free radicals and reduce oxidative stress [2]. Phytic acid dodecasodium salt hydrate chelation of metal ions (such as iron) can reduce metal-catalyzed oxidative reactions [3]. Phytic acid dodecasodium salt hydrate exhibits anticancer and antioxidant properties [4].

In MC65 cells, Cell survival rate was significantly improved after Phytic acid dodecasodium salt hydrate (100μM; 4d) treatment [5]. In HepG2 cells, Phytic acid dodecasodium salt hydrate (0.1-4mM; 24-48h) treatment inhibited cell growth [6].

In dextran sulfate sodium (DSS)-induced UC mice model, Phytic acid dodecasodium salt hydrate (0.25g/kg; ig; 14d) treatment improved weight loss, shortened colon length, improved clinical scores, and reduced the release of proinflammatory factors (Il-1β, Il-6, and TNF-α) [7]. In a high-fat diet mice model, Phytic acid dodecasodium salt hydrate (0.25-2mg/mL; po; 13 weeks) significantly inhibited obesity and alleviated hepatic steatosis [8].

References:
[1]. Oatway L, Vasanthan T, Helm J H. Phytic acid dodecasodium salt hydrate[J]. Food Reviews International, 2001, 17(4): 419-431.
[2]. Graf E, Empson K L, Eaton J W. Phytic acid dodecasodium salt hydrate. A natural antioxidant[J]. Journal of Biological Chemistry, 1987, 262(24): 11647-11650.
[3]. Lee B J, Hendricks D G. Metal‐catalyzed oxidation of ascorbate, deoxyribose and linoleic acid as affected by Phytic acid dodecasodium salt hydrate in a model system[J]. Journal of Food Science, 1997, 62(5): 935-984.
[4]. Bloot A P M, Kalschne D L, Amaral J A S, et al. A review of Phytic acid dodecasodium salt hydrate sources, obtention, and applications[J]. Food Reviews International, 2023, 39(1): 73-92.
[5]. Anekonda T S, Wadsworth T L, Sabin R, et al. Phytic acid dodecasodium salt hydrate as a potential treatment for Alzheimer's pathology: evidence from animal and in vitro models[J]. Journal of Alzheimer’s disease, 2011, 23(1): 21-35.
[6]. Al-Fatlawi A A, Al-Fatlawi A A, Irshad M, et al. Rice bran Phytic acid dodecasodium salt hydrate induced apoptosis through regulation of Bcl-2/Bax and p53 genes in HepG2 human hepatocellular carcinoma cells[J]. Asian Pacific Journal of Cancer Prevention, 2014, 15(8): 3731-3736.
[7]. Hou X, Sang Y, Dong L. The improved effect and its mechanism of Phytic acid dodecasodium salt hydrate on DSS-induced UC mice[J]. Life sciences, 2022, 311: 121139.
[8]. Ran X, Hu G, He F, et al. Phytic acid dodecasodium salt hydrate improves hepatic steatosis, inflammation, and oxidative stress in high-fat diet (HFD)-fed mice by modulating the gut–liver axis[J]. Journal of Agricultural and Food Chemistry, 2022, 70(36): 11401-11411.

Protocol

Cell experiment [1]:

Cell lines

MC65 cells

Preparation Method

Cell viability was determined using an MTS assay and a calcium ion assay. At 90% confluence, cells were harvested from TC flasks using 0.05% trypsin/EDTA, washed twice with PBS, and seeded at 15 × 103 cells/well in 96-well plates in OPTIMem medium containing (Tet+) or (Tet-) tetracycline (1μg/mL) and excipients, or Phytic acid dodecasodium salt hydrate (100μM). Cells grown under Tet- conditions typically died after approximately 3–4 days of culture, accompanied by expression of endogenous A oligomers, whereas cells grown under Tet+ conditions remained viable during this period. Wells containing growth medium without cells served as background controls, and wells containing vehicle served as positive controls. Each treatment was replicated in 3–6 wells, and the treatment duration for each experiment was 72 hours. Absorbance was measured at a wavelength of 490nm using a Spectra Max PLUS microplate reader. The cell viability under different treatments was determined relative to the cell viability under Tet + treatment. The experiments were repeated at least three times.

Reaction Conditions

100μM; 4d

Applications

Cell survival rate was significantly improved after Phytic acid dodecasodium salt hydrate treatment.
Animal experiment [2]:

Animal models

Dextran sulfate sodium (DSS)-induced UC mice model

Preparation Method

The C57BL/6 mice were randomly divided into four groups of five mice each. The specific groups were as follows: no treatment group (NT group), Phytic acid dodecasodium salt hydrate feeding group (Phytic acid dodecasodium salt hydrate group), DSS modeling group (DSS group), and Phytic acid dodecasodium salt hydrate treatment group (Phytic acid dodecasodium salt hydrate + DSS group). The mice in the Phytic acid dodecasodium salt hydrate and Phytic acid dodecasodium salt hydrate + DSS groups were gavaged with Phytic acid dodecasodium salt hydrate (0.25g/kg/day) for 21days. After receiving Phytic acid dodecasodium salt hydrate for 14days, mice in the DSS and Phytic acid dodecasodium salt hydrate + DSS groups were given 2.5% DSS aqueous solution for 7days to develop the mouse model of UC. The body weights of all mice were recorded from day 1 of the DSS feeding. Following the completion of the DSS induction period, all mice were euthanized, and colon samples were collected for subsequent studies.

Dosage form

0.25g/kg; ig; 14d

Applications

Phytic acid dodecasodium salt hydrate treatment improved weight loss, shortened colon length, improved clinical scores, and reduced the release of proinflammatory factors (Il-1β, Il-6, and TNF-α).

References:
[1]. Anekonda T S, Wadsworth T L, Sabin R, et al. Phytic acid dodecasodium salt hydrate as a potential treatment for Alzheimer's pathology: evidence from animal and in vitro models[J]. Journal of Alzheimer’s disease, 2011, 23(1): 21-35.
[2]. Hou X, Sang Y, Dong L. The improved effect and its mechanism of Phytic acid dodecasodium salt hydrate on DSS-induced UC mice[J]. Life sciences, 2022, 311: 121139.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
123408-98-0
SMILES
O=P(O[Na])(O[Na])O[C@H]1[C@H]([C@H]([C@H](OP(O[Na])(O[Na])=O)[C@@H](OP(O[Na])(O[Na])=O)[C@@H]1OP(O[Na])(O[Na])=O)OP(O[Na])(O[Na])=O)OP(O[Na])(O[Na])=O.[x].O.[12Na]
Molecular Formula
C6H8Na12O25P6
Molecular Weight
942.833
Solubility
Water: ≥ 250 mg/mL
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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