Green CMFDA
Products are for research use only. Not for human use. We do not sell to patients.
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 1mg | $224.00 | In Stock | 1 | |
| 5mg | $955.00 | In Stock | 1 |
Citations
1 publicationsPublications from researchers using this productDescription
Green CMFDA is a cell-permeable fluorescent probe (Ex=485nm; Em=514nm) and can be used as a cell tracer[1]. Upon entering a living cell, Green CMFDA is cleaved by non-specific esterases common to living cells, producing a fluorescent compound, fluorescein, which accurately reflects changes associated with dormancy, and can be useful in laboratory experiments utilizing symbiont-bearing foraminifers[2]. Green CMFDA staining reduces the viability of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and causes changes in cell morphology, accompanied by a decrease in metabolic activity[3]. The Green CMFDA has been widely used for labeling living co-cultures, facilitating real-time imaging analysis and phagocytosis measurement[4].
References:
[1] Markovic J, Borrás C, Ortega A, et al. Glutathione is recruited into the nucleus in early phases of cell proliferation[J]. Journal of Biological Chemistry, 2007, 282(28): 20416-20424.
[2] Ross B J, Hallock P. Challenges in using CellTracker Green on foraminifers that host algal endosymbionts[J]. PeerJ, 2018, 6: e5304.
[3] Andrzejewska A, Jablonska A, Seta M, et al. Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity[J]. Stem Cell Research & Therapy, 2019, 10(1): 187.
[4] Rodriguez-Manzanet R, Sanjuan M A, Wu H Y, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice[J]. Proceedings of the National Academy of Sciences, 2010, 107(19): 8706-8711.
Protocol
Cell labelling using Green CMFDA
This protocol only provides a guideline and should be modified according to your specific needs
1. Stock and dilute solution preparation for staining of cells
(1) Prepare Green CMFDA stock solution (10mM) by dissolving Green CMFDA powder in DMSO, which was then aliquoted into appropriate volumes and stored at -20°C.
(2) Dilute the Green CMFDA stock solution with PBS to a final 1µM concentration.
NOTE: The working solution must be prepared fresh on the day of the experiment.
2. Cell Preparation
Seed cultured cells in a 6-well plate or a 60-mm culture dish at 37°C, depending on the scale of the experiment.
3. Labeling of cells and analysis
(1) Wash the cells twice with PBS.
(2) Add Green CMFDA in PBS at a final concentration of 1μM, and incubate at 37°C for 30 minutes in the dark with gentle agitation.
(3) Collect cells into a 1.5ml tube and centrifuge at 500g for 10 minutes and discard the supernatant.
(4) Resuspend cells in culture medium and analyze using flow cytometry or fluorescence microscopy.
4. Experimental Precautions
(1) Ensure that the Green CMFDA is adequately protected from light.
(2) This protocol and all reagents are for scientific research use only.
References:
[1] Rodriguez-Manzanet R, Sanjuan M A, Wu H Y, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice[J]. Proceedings of the National Academy of Sciences, 2010, 107(19): 8706-8711.
Product Documents
Biological Data
Chemical Properties
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
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