Isorhamnetin (Synonyms: 3'Omethyl Quercetin) |
Catalog No.GN10023
One-Click Copy Product Info
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Isorhamnetin is a flavonoid compound extracted from the traditional Chinese medicine Hippophae rhamnoides L., possessing various biological activities including antioxidant, anti-inflammatory, anticancer, and neuroprotective effects.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 480-19-3
Sample solution is provided at 25 µL, 10mM.
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Isorhamnetin is a flavonoid compound extracted from the traditional Chinese medicine Hippophae rhamnoides L., possessing various biological activities including antioxidant, anti-inflammatory, anticancer, and neuroprotective effects[1, 2]. Isorhamnetin exerts its effects by regulating multiple cellular signaling pathways (such as NF-κB and PI3K/AKT), and is beneficial for cardiovascular health, diabetes, and potentially neurodegenerative diseases[3].
In vitro, treatment of A549 cells with Isorhamnetin (31.6, 63.2µM) for 24h resulted in the accumulation of A549 cells in the G0/G1 phase, a decrease in the number of cells in the S phase, and induced apoptosis. Isorhamnetin upregulated the expression of apoptotic genes Bax, caspase-3, and p53, and downregulated the expression of Bcl-2, cyclin D1, and PCNA proteins[4]. Treatment of HepG2 cells with Isorhamnetin (10-100µM) enhanced the nuclear translocation of NF-E2-related factor 2 (Nrf2) in a dose- and time-dependent manner, increased antioxidant response element (ARE) reporter gene activity, and elevated the protein levels of heme oxygenase-1 (HO-1) and glutamate cysteine ligase (GCL), and increased intracellular glutathione (GSH) levels[5].
In vivo, Isorhamnetin (5mg/kg) administered via intraperitoneal injection for 7 days to rats with a chronic cardiotoxicity model significantly reduced myocardial damage, decreased serum cardiac enzyme release, and reduced myocardial vacuolization[6]. Isorhamnetin (5mg/kg) administered via intraperitoneal injection to mice with experimental stroke significantly reduced cerebral edema, improved blood-brain barrier function and neurological function, and upregulated the gene expression of tight junction proteins (including occludin, ZO-1, and claudin-5)[7].
References:
[1] Khaled R. Biological activities of isorhamnetin: A review[J]. Plantae Scientia, 2020, 3(5): 78-81.
[2] Teng B, Lu Y H, Wang Z T, et al. In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402 cells[J]. Pharmacological research, 2006, 54(3): 186-194.
[3] Mao Y, Zha Y, Zang Y, et al. Isorhamnetin improves diabetes-induced erectile dysfunction in rats through activation of the PI3K/AKT/eNOS signaling pathway[J]. Biomedicine & Pharmacotherapy, 2024, 177: 116987.
[4] Li Q, Ren F Q, Yang C L, et al. Anti-proliferation effects of isorhamnetin on lung cancer cells in vitro and in vivo[J]. Asian Pacific Journal of Cancer Prevention, 2015, 16(7): 3035-3042.
[5] Yang J H, Shin B Y, Han J Y, et al. Isorhamnetin protects against oxidative stress by activating Nrf2 and inducing the expression of its target genes[J]. Toxicology and applied pharmacology, 2014, 274(2): 293-301.
[6] Sun J, Sun G, Meng X, et al. Isorhamnetin protects against doxorubicin-induced cardiotoxicity in vivo and in vitro[J]. PloS one, 2013, 8(5): e64526.
[7] Zhao J J, Song J Q, Pan S Y, et al. Treatment with isorhamnetin protects the brain against ischemic injury in mice[J]. Neurochemical Research, 2016, 41(8): 1939-1948.
| Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation Method | A549 cells were incubated with 10, 20µg/mL (31.6, 63.2µM) of Isorhamnetin for 24h. After treatment, cells were washed with PBS and fixed in ice-cold 75% ethano. Cellular DNA was stained with 0.05% propidium iodide (PI) for 20min at 4°C in darkness. The cell cycle distribution and apoptotic cells were detected with FACScan. |
Reaction Conditions | 31.6, 63.2µM; 24h |
Applications | Isorhamnetin treatment resulted in an accumulation of A549 cellsin the G0/G1 phase with concomitant losses in the S phase. |
| Animal experiment [2]: | |
Animal models | Sprague-Dawley rats |
Preparation Method | Rats were randomly assigned to four groups. (1) Control group: rats were intraperitoneally (i.p.) injected with saline; (2) Doxorubicin (Dox) group: rats were treated with Dox i.p. with a dose of 3mg/kg every other day for a cumulative dose of 9mg/kg, as previously described; (3) Isorhamnetin group (Iso): rats were pretreated with Isorhamnetin at a dose of 5mg/kg i.p. every day for 6 days; (4) Isorhamnetin doxorubicin group: rats were treated with Isorhamnetin at a dose of 5mg/kg i.p. prior to Dox for 7 days. After 28 days, the rats were then sacrificed and the hearts were removed rapidly. The relative heart weight index (heart weight-to-body weight ratio) was determined. Blood was collected and centrifuged. The serum samples were assayed for LDH, CK, and AST activities. The left ventricles of the hearts were used for histopathological examination and other analyses. |
Dosage form | 5mg/kg; 7 days; i.p. |
Applications | Daily pretreatment with Isorhamnetin (5mg/kg, i.p.) for 7 days was found to reduce Dox-induced myocardial damage significantly, including the decline of cardiac index, decrease in the release of serum cardiac enzymes and amelioration of heart vacuolation. |
References: | |
| Cas No. | 480-19-3 | SDF | |
| Synonyms | 3'Omethyl Quercetin | ||
| Chemical Name | 3,5,7-trihydroxy-2-(4-hydroxy-3-methoxyphenyl)chromen-4-one | ||
| Canonical SMILES | COC1=C(C=CC(=C1)C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O)O | ||
| Formula | C16H12O7 | M.Wt | 316.27 |
| Solubility | ≥ 31.8 mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1619 mL | 15.8093 mL | 31.6186 mL |
| 5 mM | 632.4 μL | 3.1619 mL | 6.3237 mL |
| 10 mM | 316.2 μL | 1.5809 mL | 3.1619 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)