J 147 |
Catalog No.GC10988
One-Click Copy Product Info
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reduces soluble Aβ40 and Aβ42 levels
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1146963-51-0
Sample solution is provided at 25 µL, 10mM.
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J-147 is a broad spectrum neuroprotective phenyl hydrazide compound(EC50=60-115 nM) with significant neurotrophic properties related to the induction of brain-derived neurotrophic factor (BDNF).IC50 value: 60-115 nM in vitro assay [1]Target: J-147 has been shown to promotes HT22 and primary cell survival in a dose-dependent manner (EC50 value range of 0.06 – 0.115 μM) when applied to cell exposed to the mitochondrial neurotoxin, iodoacetic acid (IAA), toxicity mediated by the excitatory amino acid glutamate, which causes HT22 cell death by an oxytosis mechanism. In the preclinical in vitro development assays, the EC50 value for J-147-induced cell survival is 25 nM. J-147 was metabolically unstable (highly metabolized) with only 12% of parent remaining after incubation with rat microsomes (phase 1 metabolism).
References:
[1]. Lapchak PA, et al. J-147 a Novel Hydrazide Lead Compound to Treat Neurodegeneration: CeeToxTM Safety and Genotoxicity Analysis. J Neurol Neurophysiol. 2013 Aug;4(3). pii: 158.
| Cas No. | 1146963-51-0 | SDF | |
| Chemical Name | (Z)-N-(2,4-dimethylphenyl)-2,2,2-trifluoro-N'-(3-methoxybenzylidene)acetohydrazide | ||
| Canonical SMILES | FC(F)(C(N(C1=CC=C(C)C=C1C)/N=C\C2=CC=CC(OC)=C2)=O)F | ||
| Formula | C18H17F3N2O2 | M.Wt | 350.33 |
| Solubility | ≥ 16.65mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8545 mL | 14.2723 mL | 28.5445 mL |
| 5 mM | 570.9 μL | 2.8545 mL | 5.7089 mL |
| 10 mM | 285.4 μL | 1.4272 mL | 2.8545 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 32 reference(s) in Google Scholar.)