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2,4-DPD (Synonyms: 2,4-Diethylpyridine dicarboxylate)

カタログ番号GC16195

ジエチル ピリジン-2,4-ジカルブは、強力なプロリル 4-ヒドロキシラーゼ指向のプロインヒビターです。

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2,4-DPD 化学構造

Cas No.: 41438-38-4

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

2,4-DPD is a cell permeable, competitive inhibitor of the oxygen-sensing enzyme HIF-α prolyl hydroxylase (HIF-PH) [1].

Hypoxia-inducible factor (HIF) is a transcription factor with a key role in cellular responses to hypoxia in a variety of organisms. The HIF system plays an important role in angiogenesis, erythropoiesis, energy utilization, glucose/energy metabolism, tumour development, and ischaemic/hypoxic disease. Genetic or pharmacological inactivation of the HIF hydroxylases results in a constitutive activation of the HIF pathway with little or even absent regulation by oxygen remaining. The oxygen-sensing enzyme HIF-α prolyl hydroxylase catalyzes hydroxylation of specific prolyl and asparaginyl residues in the regulatory HIF-α subunits [2].

Exposure to 2,4-DPD limited prolyl 4-hydroxylase activity in C. elegans., where their esters are hydrolyzed to form competitors of a -ketoglutarate. 2,4-DPD showed dramatic effects among the progeny. When exposed to a high level of 2,4-DPD (2.7 mM), all progeny died regardless of genotype of C. elegans. The dead embryos arrested at the twofold stage [1].

References:
[1] Friedman L, Higgin J J, Moulder G, et al.  Prolyl 4-hydroxylase is required for viability and morphogenesis in Caenorhabditis elegans[J]. Proceedings of the National Academy of Sciences, 2000, 97(9): 4736-4741.
[2] Schofield C J, Ratcliffe P J.  Signalling hypoxia by HIF hydroxylases[J]. Biochemical and biophysical research communications, 2005, 338(1): 617-626.

Chemical Properties

Cas No. 41438-38-4 SDF
同義語 2,4-Diethylpyridine dicarboxylate
Chemical Name 2,4-pyridinedicarboxylic acid, diethyl ester
Canonical SMILES O=C(OCC)C1=NC=CC(C(OCC)=O)=C1
Formula C11H13NO4 M.Wt 223.2
溶解度 ≤50mg/ml in ethanol;20mg/ml in DMSO;30mg/ml in dimethyl formamide Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.4803 mL 22.4014 mL 44.8029 mL
5 mM 0.8961 mL 4.4803 mL 8.9606 mL
10 mM 0.448 mL 2.2401 mL 4.4803 mL
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