Adenosine 5′-diphosphoribose sodium (Synonyms: ADP ribose sodium) |
カタログ番号GC19729
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Adenosine 5′-diphosphoribose sodiumはヌクレオチド誘導体であり、Transient receptor potential melastatin 2 (TRPM2)の拮抗剤であり、細胞で様々な代謝経路で重要な役割を果たしている。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68414-18-6
Sample solution is provided at 25 µL, 10mM.
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Adenosine 5′-diphosphoribose sodiumはヌクレオチド誘導体であり、Transient receptor potential melastatin 2 (TRPM2)の拮抗剤であり、細胞で様々な代謝経路で重要な役割を果たしている[1]。Adenosine 5′-diphosphoribose sodiumは、細胞内シグナル伝達経路を調節でき、細胞の増殖、分化とアポトーシスなどのプロセスを影響できる[2]。Adenosine 5′-diphosphoribose sodiumは細胞ストレス反応で肝心な役割をも果たし、細胞が酸化ストレスなどの副作用を対応できるよう手助けをする[3]。Adenosine 5′-diphosphoribose sodiumは細胞免疫反応と緊密に関連しており、免疫細胞活性の調節に関与している[4]。
体外で、Adenosine 5′-diphosphoribose sodium (100μM)でRIN-5F細胞を処理した結果、PLC-IP3経路を通じて、細胞内カルシウムイオン(Ca²⁺)の濃度を増やす[5]。Adenosine 5′-diphosphoribose sodium (1mM)で小膠細胞を処理した結果、Ca²⁺とMg²⁺の流入を誘発する[6]。
References:
[1] Fliegert R, Bauche A, Wolf Pérez AM, et al. 2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist. Nat Chem Biol. 2017 Sep;13(9):1036-1044.
[2] Vyas S, Chang P. New PARP targets for cancer therapy. Nat Rev Cancer. 2014 Jul;14(7):502-9.
[3] Dousa TP, Chini EN, Beers KW. Adenine nucleotide diphosphates: emerging second messengers acting via intracellular Ca2+ release. Am J Physiol. 1996 Oct;271(4 Pt 1):C1007-24.
[4] Ji D, Luo ZW, Ovcjak A, et al. Role of TRPM2 in brain tumours and potential as a drug target. Acta Pharmacol Sin. 2022 Apr;43(4):759-770.
[5] Ishii M, Shimizu S, Hagiwara T, et al. Extracellular-added ADP-ribose increases intracellular free Ca2+ concentration through Ca2+ release from stores, but not through TRPM2-mediated Ca2+ entry, in rat beta-cell line RIN-5F. J Pharmacol Sci. 2006 Jun;101(2):174-8.
[6] Kraft R, Grimm C, Grosse K, et al. Hydrogen peroxide and ADP-ribose induce TRPM2-mediated calcium influx and cation currents in microglia. Am J Physiol Cell Physiol. 2004 Jan;286(1):C129-37.
| 細胞実験[1]: | |
細胞株 | RIN-5F細胞（ラット膵臓β-細胞株） |
準備方法 | 37℃で、10%のウシ胎児血清、100U/mlのペニシリンと100μg/mlのストレプトマイシンを含有するRPMI 1640培地でRIN-5F細胞を培養した。細胞を細胞外添加のAdenosine 5′-diphosphoribose sodium (100μM)で10-20秒処理した。 |
反応条件 | 100μM；10–20秒 |
アプリケーション | 細胞外Adenosine 5′-diphosphoribose sodiumは、タプシガルギン感受性Ca²⁺貯蔵庫からのCa²⁺放出を通じて、細胞内遊離の濃度で一過性の増加を誘発した。 |
References: | |
| Cas No. | 68414-18-6 | SDF | |
| 同義語 | ADP ribose sodium | ||
| Canonical SMILES | O[C@H]([C@@H]1O)[C@@H](O[C@@H]1COP(OP(OC[C@@H](O)[C@@H](O)[C@@H](O)C=O)([O-])=O)(O)=O)N2C(N=CN=C3N)=C3N=C2.[Na+] | ||
| Formula | C₁₅H₂₂N₅NaO₁₄P₂ | M.Wt | 581.3 |
| 溶解度 | Water : 125 mg/mL (215.04 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7203 mL | 8.6014 mL | 17.2028 mL |
| 5 mM | 344.1 μL | 1.7203 mL | 3.4406 mL |
| 10 mM | 172 μL | 860.1 μL | 1.7203 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)