DPPH (Synonyms: 2,2-Diphenyl-1-Picrylhydrazyl DPPH radical) |
Catalog No.GC19475
One-Click Copy Product Info
|
DPPH (1,1-diphenyl2-picrylhydrazyl)은 전체 분자에 대한 여분의 전자 탈로화로 인해 안정한 자유 라디칼이다.DPPH검사는 가장 잘 알려진, 자주 사용되고 정확한 방법 중 하나이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1898-66-4
Sample solution is provided at 25 µL, 10mM.
DPPH (1,1-diphenyl2-picrylhydrazyl)은 전체 분자에 대한 여분의 전자 탈로화로 인해 안정한 자유 라디칼이다.DPPH검사는 가장 잘 알려진, 자주 사용되고 정확한 방법 중 하나이다. delocalization으로 끈끈한 보라색을 일으키고 λmax는 약 520 nm 정도이다. DPPH 용액을 보랏빛 색깔이 연황색으로 작용하는 기판과 혼합하면 보라색이 옅은 노란색으로 변하면서 안정된 비라디칼 형태의 DPPH를 얻게 된다[1].
DPPH검사는 에탄올과 곡휴, 야채, 공결 아오일 아세이트, 약초, 식용 기름, 밀가루의 항산화 성질을 조사하기 위해 성공적으로 사용되었다. 이는 에탄올, 수성 아세톤, 메탄올, 수성 알코올, 벤젠을 포함한 몇 가지 다른 솔벤트 시스템에서 사용된다[2,3]. DPPH검사은 시스테인, 글루타치온, 아스코르브산, 토코페롤, 폴리히드록시 방향족 화합물의 항산화 분석에 편리한 방법이다[4], 또한 올리브 오일, 과일, 주스, 와인에도 사용된다[5].
References:[1]. Szabo M, Idioiu C, Chambre D, et al. Improved DPPH determination for antioxidant activity spectrophotometric assay[J]. Chemical Papers, 2007, 61(3): 214-216.[2]. Yu L. Free radical scavenging properties of conjugated linoleic acids[J]. Journal of Agricultural and Food Chemistry, 2001, 49(7): 3452-3456.[3]. Parry J, Su L, Luther M, et al. Fatty acid composition and antioxidant properties of cold-pressed marionberry, boysenberry, red raspberry, and blueberry seed oils[J]. Journal of agricultural and food chemistry, 2005, 53(3): 566-573.[4]. Nishizawa M, Kohno M, Nishimura M, et al. Non-reductive scavenging of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) by peroxyradical: a useful method for quantitative analysis of peroxyradical[J]. Chemical and Pharmaceutical bulletin, 2005, 53(6): 714-716.[5]. Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems[J]. Food science and technology international, 2002, 8(3): 121-137.
DPPH 검사[1]
추출물의 DPPH 자유기 제거 활동을 분석했습니다. DPPH 용액(40 μg/mL)은 메탄올에 준비되었습니다. 마이크로 티터 플레이트의 각 칸에 적당한 농도(1000, 500, 100, 50, 10 μg/mL)의 20 μL 표본(3회 반복)과 180 μL의 DPPH 용액을 추가했습니다. 음성 대조군으로 메탄올을 샘플 대신 사용했습니다. BHA, BHT, 비타민C(AA)를 양성 대조군으로 사용했습니다. 반응 판은 37℃의 어두운 곳에 30분간 유지한 후 Multiskan Sky Thermo Scientific 마이크로 티터 판독기를 사용하여 517 nm에서 흡광도를 측정햤습니다. 시험 샘플에서 DPPH 자유기의 억제율은 다음 공식을 사용하여 계산하고 백분율(%)로 표현했습니다:
Ab는 음성 대조군(샘플 없음)의 흡광도이고 As는 다양한 농도의 샘플과 양성 대조군의 흡광도입니다. 결과는 DPPH 자유기 억제율의 평균 백분율 ± 표준 오차로 제시됩니다. 이 프로토콜은 지침을 제공하고 사용자의 구체적인 요구에 따라 수정되어야 합니다.
참고문헌:
[1]. Mandić M R, Oalđe M M, Lunić T M, et al. Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia[J]. PloS one, 2021, 16(2): e0246810.
| Cas No. | 1898-66-4 | SDF | |
| Synonyms | 2,2-Diphenyl-1-Picrylhydrazyl DPPH radical | ||
| Chemical Name | 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl | ||
| Canonical SMILES | O=[N+](C1=C(C([N+]([O-])=O)=CC([N+]([O-])=O)=C1)[N]N(C2=CC=CC=C2)C3=CC=CC=C3)[O-] | ||
| Formula | C18H12N5O6 | M.Wt | 394.3 |
| Solubility | 10mg/mL in DMF, 10mg/mL in Ethanol, 79mg/mL in DMSO | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5361 mL | 12.6807 mL | 25.3614 mL |
| 5 mM | 507.2 μL | 2.5361 mL | 5.0723 mL |
| 10 mM | 253.6 μL | 1.2681 mL | 2.5361 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
D Free-radical scavengabilities of ascorbic acid, PADM, and AgNP–PADM hydrogels in range 50–250μg mL^−1. E IC50 values of ascorbic acid, PADM, and AgNP–PADM hydrogel groups IC50: the 50% inhibiting concentration
Then, the ascorbic acid, the PADM hydrogel, and the AgNP–PADM hydrogel prepared using the 80-μg mL^-1 AgNP solution were added to a 0.4 mM DPPH(GlpBio) anhydrous ethanol solution, and the solution was stored in the dark for 10 min.
Biomater Res 26.1 (2022): 36. PMID: 35879746 IF: 11.2997 -
Related Biological Data
The free radical scavenging ratio was determined by the DPPH method. CNMs at different MIC levels were mixed with DPPH solution. The mixtures were shaken well and put in the dark at room temperature for 30 min.
CNMs at different MIC levels were mixed with DPPH(GlpBio) solution. The mixtures were shaken well and put in the dark at room temperature for 30 min.
Front Immunol 12 (2022): 5955. PMID: 35126369 IF: 8.7864
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)