Putrescine |
Catalog No.GC19608
One-Click Copy Product Info
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Putrescine (polyamines, spermidine and spermine) are organic cations of low molecular weight, it have important roles in cellular growth, cell differentiation, modulation of enzyme activities, regulation of ion channels and in the control of DNA and protein synthesis.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 110-60-1
Sample solution is provided at 25 µL, 10mM.
Putrescine (polyamines, spermidine and spermine) are organic cations of low molecular weight, it have important roles in cellular growth, cell differentiation, modulation of enzyme activities, regulation of ion channels and in the control of DNA and protein synthesis[1-2].
Putrescine(100 ng/ml) promoted induction of immature bone marrow derived DCs (BMDCs), along with the increased phagocytosis and migration, and altered cytokine secretion in immature BMDCs [3]. Putrescine(0.5mM) improved the mitochondrial function of both oocyte and CCs via antioxygenation and the regulation of glucose metabolism [4-5]. Putrescine(100 μM) shifts L-arginine-metabolism related-genes on BALB/c macrophages and affects infection by L. amazonensis[6].
Putrescine produces antidepressant-like effects. Putrescine (1-10 mg/kg, i.p) significantly reduced the immobility time both in the mouse forced swimming test (FST) and in the tail suspension test (TST), two models predictive of antidepressant activity [7]. Putrescine(100 μM;24h) elicits ROS-dependent activation of the salicylic acid pathway in Arabidopsis thaliana[8].
References:
[1]. Park MH, Igarashi K. Polyamines and their metabolites as diagnostic markers of human diseases. Biomol Ther (Seoul). 2013 Jan;21(1):1-9. doi: 10.4062/biomolther.2012.097. PMID: 24009852; PMCID: PMC3762300.
[2]. Bird J, Mohd-Hidir S,et,al. Putrescine--a potent endogenous anti-inflammatory substance in inflammatory exudates. Agents Actions. 1983 Jun;13(4):342-7. doi: 10.1007/BF01971486. PMID: 6613748.
[3]. Huang P, Wang M, et,al. Putrescine accelerates the differentiation of bone marrow derived dendritic cells via inhibiting phosphorylation of STAT3 at Tyr705. Int Immunopharmacol. 2023 Mar;116:109739. doi: 10.1016/j.intimp.2023.109739. Epub 2023 Jan 26. PMID: 36706590.
[4]. Xu W, Li L, et,al. Putrescine delays postovulatory aging of mouse oocytes by upregulating PDK4 expression and improving mitochondrial activity. Aging (Albany NY). 2018 Dec 16;10(12):4093-4106. doi: 10.18632/aging.101699. PMID: 30554191; PMCID: PMC6326651.
[5]. Tao Y, Tartia A, et,al. Can peri-ovulatory putrescine supplementation improve egg quality in older infertile women? J Assist Reprod Genet. 2019 Mar;36(3):395-402. doi: 10.1007/s10815-018-1327-x. Epub 2018 Nov 22. PMID: 30467617; PMCID: PMC6439130.
[6]. Zanatta JM, Acuña SM, et,al. Putrescine supplementation shifts macrophage L-arginine metabolism related-genes reducing Leishmania amazonensis infection. PLoS One. 2023 Mar 31;18(3):e0283696. doi: 10.1371/journal.pone.0283696. PMID: 37000792; PMCID: PMC10065296.
[7]. Zomkowski AD, Santos AR, et,al. Putrescine produces antidepressant-like effects in the forced swimming test and in the tail suspension test in mice. Prog Neuropsychopharmacol Biol Psychiatry. 2006 Dec 30;30(8):1419-25. doi: 10.1016/j.pnpbp.2006.05.016. Epub 2006 Jul 5. PMID: 16822602.
[8]. Liu C, Atanasov KE, et,al. Putrescine elicits ROS-dependent activation of the salicylic acid pathway in Arabidopsis thaliana. Plant Cell Environ. 2020 Nov;43(11):2755-2768. doi: 10.1111/pce.13874. Epub 2020 Sep 14. PMID: 32839979.
| Cell experiment [1]: | |
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Cell lines |
Bone marrow-derived murine dendritic cells |
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Preparation Method |
Cells were treated with putrescine and transwell experiments were performed. |
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Reaction Conditions |
100 ng/ml |
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Applications |
Putrescine enhanced immature migration of DCs. Putrescine promoted the induction of bone marrow cells to immature DCs. |
| Animal experiment [2]: | |
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Animal models |
Swiss mice |
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Preparation Method |
Putrescine injected mice intraperitoneally and then observed and measured forced swimming test (FST) . The forced swimming test was conducted on mice, each of which was placed in an open cylindrical container to swim. A mouse was considered immobile when it stopped struggling and floated passively in the water, making only minimal movements to keep its head above the surface. |
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Dosage form |
1–10 mg/kg; i.p; administered 30 min before the test |
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Applications |
Putrescine significantly decreased the immobility time in the forced swimming test (FST). |
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References: |
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| Cas No. | 110-60-1 | SDF | |
| Canonical SMILES | NCCCCN | ||
| Formula | C4H12N2 | M.Wt | 88.15 |
| Solubility | >139.9 mg/mL in DMSO; >42.3 mg/mL in EtOH; >9.3 mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 11.3443 mL | 56.7215 mL | 113.443 mL |
| 5 mM | 2.2689 mL | 11.3443 mL | 22.6886 mL |
| 10 mM | 1.1344 mL | 5.6721 mL | 11.3443 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)