SBFI AM (Synonyms: Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester) |
Catalog No.GC44876
One-Click Copy Product Info
|
SBFI AM is a membrane-permeant, fluorescent Na+ indicator dye.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 129423-53-6
Sample solution is provided at 25 µL, 10mM.
SBFI AM is a membrane-permeant, fluorescent Na+ indicator dye. It is selective for Na+ over K+ with Kd values of 20 and 120 mM for these ions, respectively. [1]Increasing concentration of Na+ increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm. [1] Therefore, ratio fluorometry and imaging can be performed with the same wavelengths. SBF AM is suitable for single intracellular dye injection and is typically used for high-resolution measurements of Na+ concentration in intact tissue through two-photon confocal imaging.[2]
Reference:
[1]. Minta, A., and Tsien, R.Y. Fluorescent indicators for cytosolic sodium. The Journal of Biological Chemisty 264(32), 19449-19457 (1989).
[2]. Meier, S.D., Kovalchuk, Y., and Rose, C.R. Properties of the new fluorescent Na+ indicator CoroNa Green: Comparison with SBFI and confocal Na+ imaging. Journal of Neuroscience Methods 155(2), 251-259 (2006).
| Cas No. | 129423-53-6 | SDF | |
| Synonyms | Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester | ||
| Chemical Name | 4,4’-[1,4,10-trioxa-7,13-diazacyclopentadecane-7,13-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3-benzenedicarboxylic acid 1,1’,3,3’-tetrakis[(acetyloxy)methyl] ester | ||
| Canonical SMILES | O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC(C=C3OC)=C(O2)C=C3N4CCOCCOCCN(C5=C(OC)C=C(C=C(C6=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C6)O7)C7=C5)CCOCC4 | ||
| Formula | C56H58N2O23 | M.Wt | 1127.1 |
| Solubility | Methanol: Soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 887.2 μL | 4.4362 mL | 8.8723 mL |
| 5 mM | 177.4 μL | 887.2 μL | 1.7745 mL |
| 10 mM | 88.7 μL | 443.6 μL | 887.2 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)