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SMIP004

Catalog No.GC16055 Copy One-Click Copy Product Info

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SMIP004 Chemical Structure

Cas No.: 143360-00-3

Size Price Stock Qty
10mM (in 1mL DMSO)
$117.00
In stock
2mg
$83.00
In stock
5mg
$125.00
In stock
10mg
$189.00
In stock
25mg
$340.00
In stock
50mg
$514.00
In stock

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Sample solution is provided at 25 µL, 10mM.



Description of SMIP004

IC50 Value: 1.09 uM (MTT assay in LNCaP-S14 cells) [1] SMIP004 (N-(4-butyl-2-methyl-phenyl) acetamide) is a novel inducer of cancer-cell selective apoptosis of human prostate cancer cells. Unlike SMIP001, SMIP004 was found to downregulate SKP2 and to stabilize p27, although neither SMIP is a proteasome inhibitor. in vitro: Whereas SMIP012 and 016 were moderately toxic in normal fibroblasts, SMIPs 001 and 004 showed substantial cancer cell specificity being at least five times more potent in LNCaP-S14 than in IMR90 cells , treatment with either MG132 or SMIP004 increased p27 half-life to > 6 h [1]. Both SMIP001 and 004 led to a strong increase in the recruitment of p27 to CDK2, while SMIP001 also slightly increased coprecipitation of p21 (Figure 6c). SMIP004 also reduced the amounts of cyclins E and A retrieved with CDK2. This was paralleled by a marked downregulation of cyclins E and A upon SMIP004 treatment. SMIP004 decreased the levels of positive cell cycle regulators, upregulated cyclin-dependent kinase inhibitors, and resulted in G1 arrest, inhibition of colony formation in soft agar, and cell death [2]. in vivo: SMIP004 potently inhibits the growth of prostate and breast cancer xenografts in mice [2]. Clinical trial: N/A

Chemical Properties of SMIP004

Cas No. 143360-00-3 SDF
Chemical Name N-(4-butyl-2-methylphenyl)acetamide
Canonical SMILES CCCCC1=CC(=C(C=C1)NC(=O)C)C
Formula C13H19NO M.Wt 205.3
Solubility ≥ 20.5 mg/mL in DMSO, ≥ 53.6 mg/mL in EtOH with ultrasonic Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of SMIP004

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1 mg 5 mg 10 mg
1 mM 4.8709 mL 24.3546 mL 48.7092 mL
5 mM 974.2 μL 4.8709 mL 9.7418 mL
10 mM 487.1 μL 2.4355 mL 4.8709 mL
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In vivo Formulation Calculator (Clear solution) of SMIP004

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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