GLPBio

Deoxyshikonin

Catalog No.: GC38767Purity:>99.50%
COASDSData Sheet
La desoxishikonina se aÍsla de Lithospermum erythrorhizon Sieb con actividad antitumoral.

Los productos son exclusivamente para uso en investigación. No aptos para uso humano. No vendemos a pacientes.

Deoxyshikonin
Cas No.: 43043-74-9
10mM (in 1mL DMSO)In Stock
$168.00
1
1mgIn Stock
$56.00
1
5mgIn Stock
$112.00
1
10mgIn Stock
$174.00
1
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Citas (Citations)

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    Nature
    641, 529–536 (2025)
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    628, 630–638 (2024)
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    388(6745) (2025)
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    387(6739) (2025)
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    387(6734) (2025)
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    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 273–287 (2023)
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    31, 1291–1307 (2021)

Descripción (Description)

Deoxyshikonin is a natural product isolated from Lithospermum erythrorhizon Sieb with antitumor activity[1]. Deoxyshikonin has proangiogenesis effect by increasing the expression of VEGF-C and VEGF-A mRNA in human microvascular endothelial cells–dermal lymphatic (HMVEC-dLy), promoting HIF-1α and HIF-1β subunit interaction and binding to specific DNA sequences targeted by HIF[2]. Deoxyshikonin is also an antibacterial agent against methicillin-resistant S. aureus (MRSA) and S. pneumonia[3]. Deoxyshikonin is usually used in research related to tumors and infections[4][5].

In vitro, Deoxyshikonin (2.5-20μM; 24h) dose-dependently reduced viability, induced sub-G1 arrest, and triggered apoptosis in human osteosarcoma U2OS and HOS cells[6].

In vivo, Deoxyshikonin (10mg/kg/day; 21 days; topical) shortened telogen and prolonged anagen, increased hair-follicle density, diameter and length, elevated VEGF/IGF-1, and reduced TGF-β1 in depilated alopecia areata C57BL/6 mice[7].

References:
[1] Zhu Y, Zhong Y, Long X, et al. Deoxyshikonin isolated from Arnebia euchroma inhibits colorectal cancer by down-regulating the PI3K/Akt/mTOR pathway. Pharm Biol. 2019;57(1):412-423.
[2] Prangsaengtong O, Park JY, Inujima A, Igarashi Y, Shibahara N, Koizumi K. Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1α Expression and Nuclear Translocation by Deoxyshikonin. Evid Based Complement Alternat Med. 2013;2013:148297.
[3] Zhang S, Wang J, Xu W, et al. Antibacterial effects of Traditional Chinese Medicine monomers against Streptococcus pneumoniae via inhibiting pneumococcal histidine kinase (VicK). Front Microbiol. 2015;6:479.
[4] Zhang S, Wang Y. Deoxyshikonin inhibits cisplatin resistance of non-small-cell lung cancer cells by repressing Akt-mediated ABCB1 expression and function. J Biochem Mol Toxicol. 2020;34(10):e22560.
[5] Cho WK, Ma JY. Deoxyshikonin Inhibits Influenza A Virus Infection at an Early Stage. Int J Mol Sci. 2025;26(17):8158.
[6] Hsieh MC, Hsieh YH, Chou CH, et al. Apoptotic effect and cell arrest of deoxyshikonin in human osteosarcoma cells through the p38 pathway. J Cell Mol Med. 2023;27(11):1592-1602.
[7] Li Y, Mu Y, Chen X, et al. Deoxyshikonin from Arnebiae Radix promotes hair growth by targeting the Wnt/β-catenin signaling pathway. Phytomedicine. 2025;140:156590.

Protocolo (Protocol)

Cell experiment [1]:

Cell lines

 Human osteosarcoma U2OS and HOS cells

Preparation Method

 U2OS cells (osteogenic sarcoma; human, 15-year-old female) were cultured in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 5mL of glutamine. HOS cells (human osteosarcoma; 13-year-old female) were cultured in Eagle's MEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 5mL glutamine. Cells were cultured at 37°C in a humidified atmosphere of 5% CO2 in an incubator and treated with Deoxyshikonin (2.5, 5, 10 and 20μM) for 24h. MTT colorimetric assay was performed to examine cell viability. FITC Annexin V Apoptosis Detection Kit was used to determine the apoptotic effect and flow cytometry was performed to analyse cell cycles.

Reaction Conditions

 2.5-20μM; 24h

Applications

 Deoxyshikonin dose-dependently reduced viability, induced sub-G1 arrest, and triggered apoptosis in human osteosarcoma U2OS and HOS cells.
Animal experiment [2]:

Animal models

 C57BL/6 mice

Preparation Method

 C57BL/6 mice (age=6 weeks, male, weight=21±2g) were kept at 23±2℃ in 12h dark/light cycle and 55% humidity with ad libitum access to chow and water. The alopecia mice model was established by topically applying depilatory paste to a 6cm² dorsal skin area using a cotton swab once daily for 21 days. Skin color and hair growth were monitored daily, and photographs were taken to document treatment effects over time. After hair removal, Deoxyshikonin (10mg/kg) were topically applied to mice. On day 14, after the depilator application, regrown hairs were randomly plucked from each mouse to measure average hair length. On day 21, a 1cm diameter circular skin sample was biopsied from the depilated area on each mouse’s back using a biopsy punch. Furthermore, hairs were scraped off, and their total weight was measured. Moreover, the lengths of 10 randomly selected hairs from the scraped sample were measured using a vernier caliper. The skin tissue content of TGF-β1, VEGF, and IGF-1 in alopecia mice was assessed by ELISA kit.

Dosage form

 10mg/kg/day; 21 days; topical

Applications

 Deoxyshikonin shortened telogen and prolonged anagen, increased hair-follicle density, diameter and length, elevated VEGF/IGF-1, and reduced TGF-β1 in depilated alopecia areata C57BL/6 mice.

References:
[1] Hsieh MC, Hsieh YH, Chou CH, et al. Apoptotic effect and cell arrest of deoxyshikonin in human osteosarcoma cells through the p38 pathway. J Cell Mol Med. 2023;27(11):1592-1602.
[2] Li Y, Mu Y, Chen X, et al. Deoxyshikonin from Arnebiae Radix promotes hair growth by targeting the Wnt/β-catenin signaling pathway. Phytomedicine. 2025;140:156590.

Documentos del producto (Product Documents)

Purity:>99.50%Appearance:A solid
COASDSData Sheet

Propiedades químicas (Chemical Properties)

N.º CAS
43043-74-9
SMILES
O=C1C(CC/C=C(C)/C)=CC(C2=C1C(O)=CC=C2O)=O
Fórmula molecular
C16H16O4
Peso molecular
272.3
Solubilidad
DMSO : 33.33 mg/mL (122.40 mM; ultrasonic and warming and heat to 60°C)
Almacenamiento
4°C, protect from light
Consejos generales
Seleccione un disolvente adecuado según los datos de solubilidad del producto. Una vez preparada, divida la solución madre en alícuotas para evitar ciclos repetidos de congelación y descongelación.
Utilice las soluciones madre en un plazo de 6 meses si se conservan a -80°C, o de 1 mes si se conservan a -20°C.
Para mejorar la solubilidad, caliente el tubo a 37°C y soníquelo durante un periodo adecuado.
Condiciones de envío
Las muestras de evaluación se envían con acumuladores de frío. Los demás tamaños se envían a temperatura ambiente o, previa solicitud, con acumuladores de frío.

Calculadoras (Calculators)Molaridad / Dilución / Peso molecular / Conversión de unidades / Formulación in vivo / Solubilidad

g/mol

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