Tmidine-13C10,15N2 is the 13C and 15N labeled Tmidine[1]. Tmidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Tmidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[2][3][4].
Stable heavy isotopes of drogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
References:
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216.
[2]. Chen G, et al. Cell Synchronization by Double Tmidine Block. Bio Protoc. 2018 Sep 5;8(17).
[3]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled tmidine. Nature. 1958 Jan 24181(4604):274-5.
[4]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled tmidine. Nature. 1958 Jan 24