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DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Products for  DNA/RNA Synthesis

  1. Cat.No. Product Name Information
  2. GC10867 (+)-Aphidicolin Tetracyclic diterpene antibiotic
  3. GC42061 2',3'-Dideoxyadenosine 5'-triphosphate 2',3'-Dideoxyadenosine 5'-triphosphate (ddATP) is an in vitro inhibitor of reverse transcriptases from retroviruses, including HIV-1 and visna (Kis = 20 and 37 nM, respectively).
  4. GC33496 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)
  5. GC34452 3,7,4'-Trihydroxyflavone
  6. GC13510 3-AP ribonucleotide reductase inhibitor and iron chelator with antitumor activity
  7. GC31187 5-Methoxyflavone
  8. GC35166 5-Methylcytosine
  9. GC12193 6-Thio-dG telomere disrupting compound
  10. GC12777 8-Chloroadenosine Nucleoside analog, inhibits RNA synthesis
  11. GC34090 Acelarin (NUC-1031)
  12. GC16866 Actinomycin D RNA polymerase inhibitor
  13. GC17186 Acyclovir Antiviral agent
  14. GC13432 Adenine High affinity adenine receptor agonist
  15. GC11825 Adenine HCl Adenine receptor agonist
  16. GC17278 Adenine sulfate Sulfate salt form of adenine
  17. GC11559 Anisomycin JNK agonist, potent and specific
  18. GC38463 ASLAN003
  19. GC15033 Azathioprine purine synthesis and GTP-binding protein Rac1 activation inhibitor
  20. GC32838 BAY-2402234
  21. GC32333 Beaucage reagent

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