Home >> Signaling Pathways >> DNA Damage/DNA Repair >> DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Targets for  DNA/RNA Synthesis

Products for  DNA/RNA Synthesis

  1. Cat.No. Product Name Information
  2. GC19566 N-Nitrosodiethylamine  N-Nitrosodiethylamine  Chemical Structure
  3. GC10867 (+)-Aphidicolin Tetracyclic diterpene antibiotic (+)-Aphidicolin  Chemical Structure
  4. GC41695 (6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) (6R,S)-5,6,7,8-Tetrahydrofolic acid (THFA), the reduced form of folic acid, serves as a cofactor in methyltransferase reactions and is the major one-carbon carrier in one carbon metabolism. (6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride)  Chemical Structure
  5. GC41088 (6S)-Tetrahydrofolic Acid (6S)-Tetrahydrofolic acid is a diastereomer of tetrahydrofolic acid, a reduced form of folic acid that serves as a cofactor in methyltransferase reactions and is the major one-carbon carrier in one carbon metabolism. (6S)-Tetrahydrofolic Acid  Chemical Structure
  6. GC41633 (R)-Prunasin (R)-Prunasin is a cyanogenic glucoside that is the precursor to amygdalin, a bitter component found in species of the genera Prunus and Olinia. (R)-Prunasin  Chemical Structure
  7. GC41859 1,5,7-Triazabicyclo[4.4.0]dec-5-ene 1,5,7-Triazabicyclo[4.4.0]dec-5-ene is a bicyclic guanidine base that can be used as a catalyst for a variety of base-mediated organic transformations, including Michael additions, Wittig reactions, Henry reactions, and transesterification reactions. 1,5,7-Triazabicyclo[4.4.0]dec-5-ene  Chemical Structure
  8. GC63796 116-9e 116-9e  Chemical Structure
  9. GC62530 2’-O-Me-C(Bz) Phosphoramidite 2’-O-Me-C(Bz) Phosphoramidite  Chemical Structure
  10. GC62529 2’-OMe-A(Bz) Phosphoramidite 2’-OMe-A(Bz) Phosphoramidite  Chemical Structure
  11. GC62531 2’-OMe-G(ibu) Phosphoramidite 2’-OMe-G(ibu) Phosphoramidite  Chemical Structure
  12. GC42061 2',3'-Dideoxyadenosine 5'-triphosphate 2',3'-Dideoxyadenosine 5'-triphosphate (ddATP) is an in vitro inhibitor of reverse transcriptases from retroviruses, including HIV-1 and visna (Kis = 20 and 37 nM, respectively). 2',3'-Dideoxyadenosine 5'-triphosphate  Chemical Structure
  13. GC42151 2'-Deoxyguanosine 5'-monophosphate (sodium salt hydrate) 2'-Deoxyguanosine 5'-monophosphate (dGMP) is used as a substrate of guanylate kinases to generate dGDP, which in turn is phosphorylated to dGTP, a nucleotide precursor used in DNA synthesis. 2'-Deoxyguanosine 5'-monophosphate (sodium salt hydrate)  Chemical Structure
  14. GC42080 2'2'-cGAMP (sodium salt) 2'2'-cGAMP is a synthetic dinucleotide (CDN) that contains non-canonical 2'5'-phosphodiester bonds. 2'2'-cGAMP (sodium salt)  Chemical Structure
  15. GC60459 2,4-D (2,4-Dichlorophenoxyacetic acid) 2,4-D (2,4-Dichlorophenoxyacetic acid)   Chemical Structure
  16. GC33496 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)  Chemical Structure
  17. GC39527 2-O-Methylcytosine 2-O-Methylcytosine  Chemical Structure
  18. GC42197 2-Thiouridine 2-Thiouridine (s2U) is a modified nucleobase found in tRNAs that is known to stabilize U:A pairs and modestly destabilize U:G wobble pairs. 2-Thiouridine  Chemical Structure
  19. GC40618 3',4',7-Trihydroxyisoflavone 3',4',7-Trihydroxyisoflavone is a natural isoflavonoid that has antioxidant activity. 3',4',7-Trihydroxyisoflavone  Chemical Structure
  20. GC60023 3'-Deoxyuridine-5'-triphosphate 3'-Deoxyuridine-5'-triphosphate  Chemical Structure
  21. GC61862 3'-Deoxyuridine-5'-triphosphate trisodium 3'-Deoxyuridine-5'-triphosphate trisodium  Chemical Structure
  22. GC45332 3'-Dephosphocoenzyme A 3'-Dephosphocoenzyme A  Chemical Structure
  23. GC34452 3,7,4'-Trihydroxyflavone 3,7,4'-Trihydroxyflavone  Chemical Structure
  24. GC13510 3-AP ribonucleotide reductase inhibitor and iron chelator with antitumor activity 3-AP  Chemical Structure
  25. GC18853 4-isocyanato TEMPO 4-isocyanato TEMPO is a spin labeling reagent used to label the 2'-position in RNA. 4-isocyanato TEMPO  Chemical Structure
  26. GC61683 4-Methoxyphenethyl alcohol 4-Methoxyphenethyl alcohol  Chemical Structure
  27. GC62157 5’-O-DMT-2’-O-TBDMS-Ac-rC 5’-O-DMT-2’-O-TBDMS-Ac-rC  Chemical Structure
  28. GC62149 5’-O-DMT-2’-TBDMS-Uridine 5’-O-DMT-2’-TBDMS-Uridine  Chemical Structure
  29. GC62532 5’-O-DMT-3’-O-TBDMS-Ac-rC 5’-O-DMT-3’-O-TBDMS-Ac-rC  Chemical Structure
  30. GC62573 5’-O-DMT-Bz-rC 5’-O-DMT-Bz-rC  Chemical Structure
  31. GC62574 5’-O-DMT-N4-Ac-2’-F-dC 5’-O-DMT-N4-Ac-2’-F-dC  Chemical Structure
  32. GC62575 5’-O-DMT-N4-Bz-2’-F-dC 5’-O-DMT-N4-Bz-2’-F-dC  Chemical Structure
  33. GC62576 5’-O-DMT-N4-Bz-5-Me-dC 5’-O-DMT-N4-Bz-5-Me-dC  Chemical Structure
  34. GC62812 5’-O-DMT-PAC-dA 5’-O-DMT-PAC-dA  Chemical Structure
  35. GC62813 5’-O-DMT-rI 5’-O-DMT-rI  Chemical Structure
  36. GC62577 5’-O-DMT-rU 5’-O-DMT-rU  Chemical Structure
  37. GC62578 5’-O-TBDMS-Bz-dA 5’-O-TBDMS-Bz-dA  Chemical Structure
  38. GC62579 5’-O-TBDMS-dA 5’-O-TBDMS-dA  Chemical Structure
  39. GC62580 5’-O-TBDMS-dG 5’-O-TBDMS-dG  Chemical Structure
  40. GC62581 5’-O-TBDMS-dT 5’-O-TBDMS-dT  Chemical Structure
  41. GC40641 5'-Ethynyl-2'-deoxycytidine 5'-Ethynyl-2'-deoxycytidine (EdC) is a nucleoside analog that inhibits replication of the herpes simplex virus-1 (HSV-1) KOS strain (ID50 = 0.2 μg/mL). 5'-Ethynyl-2'-deoxycytidine  Chemical Structure
  42. GC61726 5'-O-DMT-N4-Ac-dC 5'-O-DMT-N4-Ac-dC  Chemical Structure
  43. GC62389 5-Iminodaunorubicin 5-Iminodaunorubicin  Chemical Structure
  44. GC31187 5-Methoxyflavone 5-Methoxyflavone  Chemical Structure
  45. GC35166 5-Methylcytosine 5-Methylcytosine  Chemical Structure
  46. GC62547 5-O-TBDMS-N4-Benzoyl-2-deoxycytidine 5-O-TBDMS-N4-Benzoyl-2-deoxycytidine  Chemical Structure
  47. GC60031 6-Azathymine 6-Azathymine  Chemical Structure
  48. GC50249 6-Hydroxy-DL-DOPA Allosteric inhibitor of RAD52; also APE1 inhibitor 6-Hydroxy-DL-DOPA  Chemical Structure
  49. GC12193 6-Thio-dG telomere disrupting compound 6-Thio-dG  Chemical Structure
  50. GC33577 7-Aminoactinomycin D (7-AAD) 7-Aminoactinomycin D (7-AAD)  Chemical Structure
  51. GC49561 7-Methylguanosine-d3 An internal standard for the quantification of 7-methylguanosine 7-Methylguanosine-d3  Chemical Structure
  52. GC12777 8-Chloroadenosine Nucleoside analog, inhibits RNA synthesis 8-Chloroadenosine  Chemical Structure
  53. GC61763 Ac-rC Phosphoramidite Ac-rC Phosphoramidite  Chemical Structure
  54. GC34090 Acelarin (NUC-1031) Acelarin (NUC-1031)  Chemical Structure
  55. GC16866 Actinomycin D

    RNA polymerase inhibitor

    Actinomycin D  Chemical Structure
  56. GC17186 Acyclovir Antiviral agent Acyclovir  Chemical Structure
  57. GC13432 Adenine High affinity adenine receptor agonist Adenine  Chemical Structure
  58. GC11825 Adenine HCl Adenine receptor agonist Adenine HCl  Chemical Structure
  59. GC17278 Adenine sulfate Sulfate salt form of adenine Adenine sulfate  Chemical Structure
  60. GC42732 Adenosine 3',5'-diphosphate (sodium salt) Adenosine 3',5'-diphosphate is an adenine nucleotide containing a phosphate group at the 3' and 5' positions of the pentose sugar ribose. Adenosine 3',5'-diphosphate (sodium salt)  Chemical Structure
  61. GC42733 Adenosine 5'-monophosphate (sodium salt hydrate) Adenosine 5'-monophosphate (AMP) is a central nucleotide with functions in metabolism and cell signaling. Adenosine 5'-monophosphate (sodium salt hydrate)  Chemical Structure
  62. GC42734 Adenosine 5'-phosphosulfate (sodium salt) Adenosine 5'-phosphosulfate is an ATP and sulfate competitive inhibitor of ATP sulfurylase in humans, S. Adenosine 5'-phosphosulfate (sodium salt)  Chemical Structure
  63. GC62470 AG-636 AG-636  Chemical Structure
  64. GC11559 Anisomycin

    JNK agonist, potent and specific

    Anisomycin  Chemical Structure
  65. GC40032 Antipain (hydrochloride)

    Antipain is a protease inhibitor originally isolated from actinomycetes.

    Antipain (hydrochloride)  Chemical Structure
  66. GC38463 ASLAN003 ASLAN003  Chemical Structure
  67. GC61821 AT-130 AT-130  Chemical Structure
  68. GC40005 Aurodox

    Aurodox is a polyketide antibiotic originally isolated from S.

    Aurodox  Chemical Structure
  69. GC60062 AV-153 free base AV-153 free base  Chemical Structure
  70. GC15033 Azathioprine purine synthesis and GTP-binding protein Rac1 activation inhibitor Azathioprine  Chemical Structure
  71. GC60616 AZT triphosphate AZT triphosphate  Chemical Structure
  72. GC60617 AZT triphosphate TEA AZT triphosphate TEA  Chemical Structure
  73. GC50656 BAY 707 BAY 707  Chemical Structure
  74. GC32838 BAY-2402234 BAY-2402234  Chemical Structure
  75. GC50572 BC-LI-0186 Leucyl-tRNA synthase (TRS)/Ras-related GTP-binding protein D (RagD) interaction inhibitor BC-LI-0186  Chemical Structure
  76. GC62863 BCH001 BCH001  Chemical Structure
  77. GC32333 Beaucage reagent Beaucage reagent  Chemical Structure
  78. GC18094 Blasticidin S HCl Antibiotic, inhibits protein synthesis Blasticidin S HCl  Chemical Structure
  79. GC60089 Bleomycin hydrochloride Bleomycin hydrochloride  Chemical Structure
  80. GC15819 Bleomycin Sulfate

    Chemotherapy agent, induces DNA strand break

    Bleomycin Sulfate  Chemical Structure
  81. GC15190 BMH-21 RNA polymerase I Inhibitior BMH-21  Chemical Structure
  82. GC50140 BRACO 19 trihydrochloride

    Telomerase inhibitor

    BRACO 19 trihydrochloride  Chemical Structure
  83. GC62128 Bractoppin Bractoppin  Chemical Structure
  84. GC32702 Branaplam (LMI070) Branaplam (LMI070)  Chemical Structure
  85. GC42978 Bromamphenicol Bromamphenicol is a dibrominated derivative of the antibiotic chloramphenicol. Bromamphenicol  Chemical Structure
  86. GC62136 BVDV-IN-1 BVDV-IN-1  Chemical Structure
  87. GC61735 Bz-rA Phosphoramidite Bz-rA Phosphoramidite  Chemical Structure
  88. GC15866 Capecitabine DNA, RNA and protein synthesis inhibitor Capecitabine  Chemical Structure
  89. GC38896 Caracemide Caracemide  Chemical Structure
  90. GC11207 Carboplatin Antitumor agent that forms platinum-DNA adducts. Carboplatin  Chemical Structure
  91. GC12748 Carmofur Cytostatic derivative of fluorouracilm,antineoplatic agent Carmofur  Chemical Structure
  92. GC19098 CeMMEC1 CeMMEC1 is an inhibitor of BRD4, and also has high affinity for TAF1, with an IC50 of 0.9 uM for TAF1, and a Kd of 1.8 uM for TAF1 (2). CeMMEC1  Chemical Structure
  93. GC19099 CeMMEC13 CeMMEC13 is a potent inhibitor of TAF1 (2) bromodomain, with an IC50 of 2.1 uM. CeMMEC13  Chemical Structure
  94. GC32250 Chebulinic acid Chebulinic acid  Chemical Structure
  95. GC18640 cis-9,10-Methyleneoctadecanoic Acid methyl ester cis-9,10-Methyleneoctadecanoic acid methyl ester is a cyclopropane fatty acid methyl ester. cis-9,10-Methyleneoctadecanoic Acid methyl ester  Chemical Structure
  96. GC10509 Cladribine Apoptosis inducer in CLL cells Cladribine  Chemical Structure
  97. GC15219 Clofarabine Antimetabolite,inhibit DNA polymerase and ribonucleotide reductase Clofarabine  Chemical Structure
  98. GC19110 COH29 COH29 is a potent ribonucleotide reductase (RNR) inhibitor with anticancer activity. COH29  Chemical Structure
  99. GC17231 CRT0044876 APE1 inhibitor, potent and selective CRT0044876  Chemical Structure
  100. GC14404 CX-5461 Pol I-mediated rRNA synthesis inhibitor CX-5461  Chemical Structure
  101. GC35761 CX-5461 dihydrochloride CX-5461 dihydrochloride  Chemical Structure

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