360A iodide |
Catalog No.GC10115
One-Click Copy Product Info
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360A iodide is a G-quadruplex stabilizer and also an effective inhibitor of telomerase activity (IC₅₀=300nM).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 737763-37-0
Sample solution is provided at 25 µL, 10mM.
360A iodide is a G-quadruplex stabilizer and also an effective inhibitor of telomerase activity (IC₅₀=300nM)[1-2]. 360A iodide can interfere with the cell cycle and induce cancer cell death[3-4].
In vitro, treatment of human glioma cell lines T98G, CB193, and U118-MG with 360A iodide (5μM) for 7-14 days. 360A iodide significantly reduced cell viability and induced cell cycle arrest and apoptosis[5]. Treatment of ATM-deficient (ATMKD) and ATM-normal human HeLa cells with 360A iodide (5μM) for 12 hours. 360A iodide significantly induced telomere instability, including sister telomere fusion, telomere loss, and telomere doublet formation[6].
References:
[1] Granotier C, Pennarun G, Riou L, et al. Preferential binding of a G-quadruplex ligand to human chromosome ends. Nucleic Acids Res. 2005 Jul 28;33(13):4182-90.
[2] Hwang IP, Mailliet P, Hossard V, et al. Investigating the Effect of Mono- and Dimeric 360A G-Quadruplex Ligands on Telomere Stability by Single Telomere Length Analysis (STELA). Molecules. 2019 Feb 6;24(3):577.
[3] Revikumar A, Kashyap V, Palollathil A, et al. Multiple G-quadruplex binding ligand induced transcriptomic map of cancer cell lines. J Cell Commun Signal. 2022 Mar;16(1):129-135.
[4] Gauthier LR, Granotier C, Hoffschir F, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40.
[5] Pennarun G, Granotier C, Gauthier LR, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28.
[6] Pennarun G, Granotier C, Hoffschir F, et al. Role of ATM in the telomere response to the G-quadruplex ligand 360A. Nucleic Acids Res. 2008 Mar;36(5):1741-54.
| Cell experiment [1]: | |
Cell lines | Human glioma cell lines (T98G, CB193, U118-MG), ALT osteosarcoma cell line (SAOS-2), and primary normal astrocytes |
Preparation Method | Cells were maintained in DMEM supplemented with 10% fetal bovine serum, 2mM glutamine, and antibiotics at 37°C under 5% CO₂. Cells were treated with 360A iodide (5μM) for 7–14 days. |
Reaction Conditions | 5µM; 7–14 days |
Applications | 360A iodide induced dose-dependent apoptosis in telomerase-positive glioma cells and ALT-positive SAOS-2 cells, preceded by S-phase arrest, metaphase prolongation, and cytokinesis defects. |
References: | |
| Cas No. | 737763-37-0 | SDF | |
| Chemical Name | (3Z,3'Z)-3,3'-((pyridine-2,6-dicarbonyl)bis(azanylylidene))bis(1-methyl-2,3-dihydroquinolin-1-ium-2-ide) dihydroiodide | ||
| Canonical SMILES | CN1=C2C=CC=CC2=C/C(C=1)=N/C(C3=NC(C(/N=C4C=C5C=CC=CC5=N(C)=C/4)=O)=CC=C3)=O.I.I | ||
| Formula | C27H23I2N5O2 | M.Wt | 703.31 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4218 mL | 7.1092 mL | 14.2185 mL |
| 5 mM | 284.4 μL | 1.4218 mL | 2.8437 mL |
| 10 mM | 142.2 μL | 710.9 μL | 1.4218 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)