Asiaticoside
Catalog No.: GN10534Purity:>98.00%Synonyms: Ba 2742, NSC 36002, NSC 166062
Asiaticoside is a pentacyclic triterpenoid glycoside extracted from Centella asiatica.
المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.
Cas No.: 16830-15-2
10mM (in 1mL DMSO)In Stock
$33.00
1
1mgIn Stock
$9.00
1
5mgIn Stock
$19.00
1
10mgIn Stock
$30.00
1
50mgIn Stock
$60.00
1
100mgIn Stock
$90.00
1
500mgIn Stock
$162.00
1
1gIn Stock
$227.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $33.00 | In Stock | 1 | |
| 1mg | $9.00 | In Stock | 1 | |
| 5mg | $19.00 | In Stock | 1 | |
| 10mg | $30.00 | In Stock | 1 | |
| 50mg | $60.00 | In Stock | 1 | |
| 100mg | $90.00 | In Stock | 1 | |
| 500mg | $162.00 | In Stock | 1 | |
| 1g | $227.00 | In Stock | 1 |
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المراجع (Citations)
1 publicationsمنشورات الباحثين الذين استخدموا هذا المنتجالوصف (Description)
Asiaticoside is a pentacyclic triterpenoid glycoside extracted from Centella asiatica [1]. Asiaticoside activates the Smad signaling pathway (specifically in a TGF-β receptor I kinase-independent manner), promoting the synthesis of type I collagen by fibroblasts, thereby strengthening skin structure and wound healing [2-3]. Asiaticoside is commonly used in wound healing and skin disease treatment [4].
In HCT116 cells, Asiaticoside (0.1-2µM; 24-72h) significantly reduced cell viability in a time- and dose-dependent manner [5]. In human brain microvascular endothelial cells, Asiaticoside (50µM; 24h) effectively attenuated Aβ1-42-induced cell growth inhibition [6].
In Swiss mice, Asiaticoside (5-40mg/kg; po; single administration) treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm [7], In Swiss mice Asiaticoside (10-20mg/kg; po; single administration) significantly shortened the immobility time of mice in the tail suspension test [8].
References:
[1]. Shukla A, Rasik A M, Jain G K, et al. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica[J]. Journal of ethnopharmacology, 1999, 65(1): 1-11.
[2]. Tang B, Zhu B, Liang Y, et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts[J]. Archives of dermatological research, 2011, 303(8): 563-572.
[3]. Lee J, Jung E, Kim Y, et al. Asiaticoside induces human collagen I synthesis through TGFβ receptor I kinase (TβRI kinase)-independent Smad signaling[J]. Planta medica, 2006, 72(04): 324-328.
[4]. Bandopadhyay S, Mandal S, Ghorai M, et al. Therapeutic properties and pharmacological activities of asiaticoside and madecassoside: A review[J]. Journal of cellular and molecular medicine, 2023, 27(5): 593-608.
[5]. Zhou X, Ke C, Lv Y, et al. Asiaticoside suppresses cell proliferation by inhibiting the NF‑κB signaling pathway in colorectal cancer[J]. International Journal of Molecular Medicine, 2020, 46(4): 1525-1537.
[6]. Song D, Jiang X, Liu Y, et al. Asiaticoside attenuates cell growth inhibition and apoptosis induced by Aβ1-42 via inhibiting the TLR4/NF-κB signaling pathway in human brain microvascular endothelial cells[J]. Frontiers in pharmacology, 2018, 9: 28.
[7]. Chen S W, Wang W J, Li W J, et al. Anxiolytic-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2006, 85(2): 339-344.
[8]. Liang X, Huang Y N, Chen S W, et al. Antidepressant-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2008, 89(3): 444-449.
البروتوكول (Protocol)
| Cell experiment [1]: | |
Cell lines | HCT116 cells |
Preparation Method | Cell viability was assessed using a CCK-8 assay. HCT116 cells in the logarithmic growth phase were digested and seeded into 96-well plates at a density of 5×103 cells/well followed by treatment with various concentrations of Asiaticoside (0.1, 0.5 and 2µM) at 37℃. Cells treated with 0.3% DMSO were used as the negative controls. Subsequently, 10% CCK-8 reagent was added to each well at different time intervals (24, 48 and 72h), and cells incubated for 2h at 37℃. The absorbance was then measured using a microplate reader at a wavelength of 450nm. |
Reaction Conditions | 0.1-2µM; 24-72h |
Applications | Asiaticoside significantly reduced cell viability in a time- and dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Swiss mice |
Preparation Method | The elevated plus-maze consists of four arms (30 × 5cm) elevated 45cm above the floor, with each arm positioned at 90° relative to the adjacent arms. The two enclosed arms had 30cm walls and to facilitate grip on the open arms these included a raised edge of 0.25cm. Open and closed arms were connected via a central area (5 × 5cm) to form a plus sign. The maze floor was constructed of black Plexiglas and the wall of the enclosed arms was constructed of clear Plexiglas. Four 25-W red fluorescent lights arranged as a cross at 100cm above the maze were used as the source of illumination. Mice (n= 9-10 per group) were randomly assigned (with a slight adjustment for matched body-weight) to eight experimental groups (vehicle-control, 0.5, 1, 2mg/kg diazepam or 5, 10, 20, 40mg/kg Asiaticoside). Drug was administration PO 60min prior to the test. Testing commenced by placing a mouse on the central platform of the maze facing an open arm. The number of entries into and the time spent on each of the two types of arms were recorded during the 5-min trial. An arm entry was defined as all four paws having crossed the dividing line between an arm and the central area. The plus-maze was thoroughly cleaned after each trial. |
Dosage form | 5-40mg/kg; po; single administration |
Applications | Asiaticoside treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm. |
References: | |
وثائق المنتج (Product Documents)
Purity:>98.00%Appearance:A solid
الخصائص الكيميائية (Chemical Properties)
رقم CAS
16830-15-2
المرادفات
Ba 2742, NSC 36002, NSC 166062
الاسم الكيميائي
Asiaticoside
SMILES
CC1CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)O)O)C)C)C2C1C)C)C(=O)OC6C(C(C(C(O6)COC7C(C(C(C(O7)CO)OC8C(C(C(C(O8)C)O)O)O)O)O)O)O)O
الصيغة الجزيئية
C48H78O19
الوزن الجزيئي
959.12
الذوبانية
≥ 44.6mg/mL in DMSO
التخزين
Store at 2-8°C, protect from light
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.
الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية
g/mol
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