Sodium laureth sulfate (Sodium lauryl polyoxyethylene ether sulfate)

Cell lines

Saccharomyces cerevisiae (yeast strain ATCC9763)

Preparation Method

S. cerevisiae cells were cultured in yeast extract-peptone-dextrose (YPD) medium at 30°C with shaking (120rpm) until mid-log phase. Cells were harvested, washed, and resuspended in sterile saline (0.85% NaCl). Cells were treated with plasma-activated water (PAW) combined with Sodium laureth sulfate at concentrations of 0.01–0.50mg/mL for 5–20min.

Reaction Conditions

0.01–0.50mg/mL; 5–20min

Applications

The combined treatment of PAW and Sodium laureth sulfate synergistically inactivated S. cerevisiae, reducing the population from 6.95 log₁₀CFU/mL to undetectable levels. Sodium laureth sulfate enhanced PAW-induced membrane damage, leading to leakage of intracellular nucleic acids and proteins.

Animal models

Albino guinea pigs

Preparation Method

Guinea pigs were treated topically with Sodium lauryl sulphate at 50mg/kg alone or in combination with Sodium laureth sulfate (50mg/kg) for 7 and 14 days. The treated skin was homogenized in sucrose solution, and enzyme activities and biochemical parameters were analyzed.

Dosage form

50mg/kg; topical application; daily for 7–14 days.

Applications

Sodium laureth sulfate treatment significantly increased the activities of lysosomal enzymes (acid phosphatase, β-glucuronidase), cytoplasmic enzymes (lactic dehydrogenase, malic dehydrogenase), and amino acid metabolism enzymes (aspartate aminotransferase, alanine aminotransferase). Sodium laureth sulfate also elevated skin amino nitrogen and sulphydryl group contents. These alterations were more pronounced when Sodium laureth sulfate was combined with nickel, indicating synergistic dermal toxicity. The study suggests that Sodium laureth sulfate induces skin irritation through membrane damage, protein denaturation, and inflammatory responses, highlighting risks for industrial workers exposed to Sodium laureth sulfate and nickel.

References:
[1] Liu X, Li Y, Zhang R, et al. Inactivation effects and mechanisms of plasma-activated water combined with sodium laureth sulfate (SLES) against Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2021 Apr;105(7):2855-2865.
[2] Mathur AK, Agarwal C, Singh A, et al. Effect of sodium lauryl sulphate and nickel alone and in combination on the skin of guinea pigs. Toxicol Lett. 1988 Sep;42(3):249-56.