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FITC, Fluorescein isothiocyanate (Synonyms: 5-FITC, FITC isomer I, 5-Isothiocyanatofluorescein)

Catalog No.GC12317

FITC, Fluorescein isothiocyanate (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines.

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FITC, Fluorescein isothiocyanate Chemical Structure

Cas No.: 3326-32-7

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10mM (in 1mL DMSO)
$39.00
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100mg
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500mg
$69.00
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1g
$118.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.

References:

[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
[2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71

Background

FITC is a derivative of fluorescein for the labeling of amines.

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].

References:
[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
[2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71

Chemical Properties

Cas No. 3326-32-7 SDF
Synonyms 5-FITC, FITC isomer I, 5-Isothiocyanatofluorescein
Chemical Name 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
Canonical SMILES S=C=NC1=CC(C(OC23C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)=O)=C2C=C1
Formula C21H11NO5S M.Wt 389.38
Solubility ≥ 38.9mg/mL in DMSO Storage Store at 2-8°C, protect from light, stored under nitrogen
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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