FlAsH-EDT2 (Synonyms: Fluorescein Arsenical Helix Binder, Lumio Green) |
Catalog No.GC43674
One-Click Copy Product Info
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FlAsH-EDT2 is a fluorescent biarsenical dye used for labeling proteins tagged with tetracysteine motifs. FlAsH-EDT2 can cross the cell membrane, making FlAsH-EDT2 suitable for labeling proteins in living cells, including both intracellular and cell surface proteins.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 212118-77-9
Sample solution is provided at 25 µL, 10mM.
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FlAsH-EDT2 is a fluorescent biarsenical dye used for labeling proteins tagged with tetracysteine motifs. FlAsH-EDT2 can cross the cell membrane, making FlAsH-EDT2 suitable for labeling proteins in living cells, including both intracellular and cell surface proteins.
References:
[1] Hoffmann C, Gaietta G, Zürn A, et al. Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc. 2010 Sep;5(10):1666-77.
Labeling proteins tagged with tetracysteine using FlAsH-EDT2[1]
This protocol only provides a guideline, and should be modified according to your specific needs.
Cell Labeling:
1. Wash the cells twice with 2ml of HBSS/glucose solution.
2. Transfer 1ml of the HBSS/glucose solution for each sample to a Falcon tube.
3. Take 50-100µl of the HBSS/glucose solution and add the HBSS/glucose solution to the FlAsH-EDT2 solution, mixing well.
4. Add the mixed solution to the Falcon tube, ensuring the solution is homogeneous.
5. Add 1ml of this solution to each culture dish, gently swirling the dish to ensure initial mixing.
6. Incubate at 37°C (with a humidified atmosphere and CO₂ as appropriate) for 1h. During the labeling process, the concentrations in the system are 500nM FlAsH-EDT2, 12.5µM EDT (1,2-ethanedithiol), and 0.1% (v/v) DMSO.
Removal of Excess and Nonspecifically Bound Dye:
7. Preparation of the washing solution: Prepare a 500mM EDT solution in DMSO.
8. Wash the cells with HBSS/glucose solution containing 250µM EDT for 10min.
9. Wash the cells 2-3 times with HBSS/glucose solution (without EDT) to reduce EDT residue.
10. Repeat the washing step 1 or 2 times to further reduce background fluorescence.
Imaging:
11. Confocal microscopy: Directly excite FlAsH using a 514nm argon laser and record its fluorescence emission.
References:
[1] Hoffmann C, Gaietta G, Zürn A, et al. Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc. 2010 Sep;5(10):1666-77.
| Cas No. | 212118-77-9 | SDF | |
| Synonyms | Fluorescein Arsenical Helix Binder, Lumio Green | ||
| Chemical Name | 4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one | ||
| Canonical SMILES | OC1=C([As]2SCCS2)C3=C(C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C([As]7SCCS7)=C6O3)C=C1 | ||
| Formula | C24H18As2O5S4 | M.Wt | 664.5 |
| Solubility | DMF: 15 mg/ml,DMSO: 20 mg/ml,DMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5049 mL | 7.5245 mL | 15.0489 mL |
| 5 mM | 301 μL | 1.5049 mL | 3.0098 mL |
| 10 mM | 150.5 μL | 752.4 μL | 1.5049 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)