6-hydroxy Chlorzoxazone
Catalog No.: GC42580Purity:>97.00%Synonyms: NSC 24955
6-hydroxy Chlorzoxazone is the primary metabolite of Chlorzoxazone in vivo. 6-hydroxy Chlorzoxazone is mainly formed through the hepatic cytochrome P450 enzyme system and is commonly used as a probe in biochemical research to quantitatively evaluate CYP2E1 activity.
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Cas No.: 1750-45-4
10mM (in 1mL DMSO)In Stock
$180.00
1
1mgIn Stock
$88.00
1
5mgIn Stock
$192.00
1
10mgIn Stock
$312.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $180.00 | In Stock | 1 | |
| 1mg | $88.00 | In Stock | 1 | |
| 5mg | $192.00 | In Stock | 1 | |
| 10mg | $312.00 | In Stock | 1 |
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Citations
1 publicationsPublications from researchers using this productDescription
6-hydroxy Chlorzoxazone is the primary metabolite of Chlorzoxazone in vivo. 6-hydroxy Chlorzoxazone is mainly formed through the hepatic cytochrome P450 enzyme system and is commonly used as a probe in biochemical research to quantitatively evaluate CYP2E1 activity. 6-hydroxy Chlorzoxazone is also involved in studies of hepatic xenobiotic metabolism, pharmacokinetics, and toxicology to elucidate metabolic pathways and drug interaction mechanisms[1-2].
References:
[1] Dreisbach AW, Ferencz N, Hopkins NE, et al. Urinary excretion of 6-hydroxychlorzoxazone as an index of CYP2E1 activity. Clin Pharmacol Ther. 1995 Nov;58(5):498-505.
[2] O'Shea D, Davis SN, Kim RB, et al. Effect of fasting and obesity in humans on the 6-hydroxylation of chlorzoxazone: a putative probe of CYP2E1 activity. Clin Pharmacol Ther. 1994 Oct;56(4):359-67.
Protocol
Determination of CYP2E1 Enzyme Activity Using 6-hydroxy Chlorzoxazone
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent and Material Preparation
(1) Standard stock solution: Dissolve 6-hydroxy Chlorzoxazone in methanol to prepare a 1mg/mL master stock solution, and store at -20°C.
(2) Incubation system: Contains 0.25mg/mL human liver microsomal protein, 50μM Chlorzoxazone, 1.15% KCl, 0.2M phosphate buffer (pH=7.4), and 5mM NADPH.
(3) Stop solution: Water/acetonitrile/formic acid (42:55:3, v/v), containing the internal standard Chlorpropamide.
2. Chromatographic and Mass Spectrometry Conditions
(1) Chromatographic column: Waters Acquity UPLC BEH C18 (50mm × 2.1mm, 1.7μm) with a VanGuard pre-column (5mm × 2.1mm, 1.7μm).
(2) Mobile phase: Phase A is 0.1% formic acid in water, Phase B is 0.1% formic acid in acetonitrile; gradient elution (5% B → 80% B, 4min), flow rate 0.4mL/min, column temperature 35°C.
3. Incubation and Sample Processing: Take 50μL of microsomal suspension and mix with 50μL of Chlorzoxazone. Add buffer and KCl, and pre-incubate for 3 minutes (37°C). Initiate the reaction by adding NADPH, incubate for 15 minutes, then add 25μL of stop solution to terminate the reaction. After cooling on ice, centrifuge (20,000×g, 10min, 4°C), and collect the supernatant for injection analysis.
Precautions:
(1) The total analysis time of this method is 5.25 minutes, making it suitable for high-throughput detection. 6-hydroxy Chlorzoxazone must be detected in negative ion mode and analyzed in two separate injections along with other metabolites.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] De Bock L, Boussery K, Colin P, et al. Development and validation of a fast and sensitive UPLC-MS/MS method for the quantification of six probe metabolites for the in vitro determination of cytochrome P450 activity. Talanta. 2012 Jan 30;89:209-16.
Product Documents
Purity:>97.00%Appearance:A solid
Chemical Properties
CAS No.
1750-45-4
Synonyms
NSC 24955
Chemical Name
5-chloro-6-hydroxy-2(3H)-benzoxazolone
SMILES
O=c1[nH]c2cc(Cl)c(O)cc2o1
Molecular Formula
C7H4ClNO3
Molecular Weight
185.6
Solubility
Slightly soluble in DMSO, Slightly soluble in Methanol
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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