Lomustine

Cell lines

L1210 leukemia cells

Preparation method

The solubility of this compound in DMSO is >11.7mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

50 μg/ml, 37°C

Applications

In L1210 leukemia cells, the radioactivity of the cyclohexyl moiety of CCNU (Lomustine) was bound almost exclusively to proteins, and that of the ethylene group was bound (at a much lower level) to both nucleic acids and proteins.

Animal models

L1210 leukemia-bearing mice

Dosage form

0.5 mg in 0.2 ml of dimethyl sulfoxide; i.p. injection

Application

In L1210 leukemia-bearing mice, the radioactivity of the cyclohexyl moiety of CCNU (Lomustine) was almost exclusively bound to cellular proteins, rather than to nucleic acids. The radioactivity of the ethylene moiety was bound to both nucleic acids and proteins of brain, liver, and leukemia cells to about the same level.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Cheng CJ, Fujimura S, Grunberger D, Weinstein IB. Interaction of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (NSC 79037) with nucleic acids and proteins in vivo and in vitro. Cancer Res. 1972 Jan;32(1):22-7.