IF 11
PMID: 42365696 ↗Hypoxanthine
Catalog No.: GC10199Purity:>99.50%Synonyms: 6-Hydroxypurine, NSC 14665, NSC 129419
Hypoxanthine is a metabolite of the purine catabolism pathway.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68-94-0
10mM (in 1mL DMSO)In Stock
$27.00
1
50mgIn Stock
$25.00
1
100mgIn Stock
$35.00
1
500mgIn Stock
$66.00
1
1gIn Stock
$105.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $27.00 | In Stock | 1 | |
| 50mg | $25.00 | In Stock | 1 | |
| 100mg | $35.00 | In Stock | 1 | |
| 500mg | $66.00 | In Stock | 1 | |
| 1g | $105.00 | In Stock | 1 |
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Citations
2 publicationsPublications from researchers using this productIF 10
PMID: 40081774 ↗Description
Hypoxanthine is a metabolite of the purine catabolism pathway[1]. Hypoxanthine can be used as rapid and sensitive bio-marker for acute cardiac ischemia at an early stage[2]. Hypoxanthine acts as a substrate for xanthine oxidase and is associated with ROS (reactive oxygen species), which contribute to vascular dysfunction and atherosclerosis[1].
Hypoxanthine (1.5, 2mM; 2h) induced ROS generation and increased cholesterol levels in HepG2 cells[1].
Hypoxanthine (200mg/kg; 8weeks; p.o.) significantly increased the cholesterol ester levels in Apoe KO mice[1]. Hypoxanthine (135mg/kg; 3d; i.v.) consistently increased the brain infarct volume in C57BL/6J mice underwent middle cerebral artery occlusion surgery[3].
References:
[1] yu H M, Kim Y J, Oh E J, et al. Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E-deficient mice and cells [J]. Journal of cellular and molecular medicine, 2016, 20(11): 2160-2172.
[2] Farthing D E, Farthing C A, Xi L. Inosine and hypoxanthine as novel biomarkers for cardiac ischemia: from bench to point-of-care [J]. Experimental biology and medicine, 2015, 240(6): 821-831.
[3] Ye J, Bi X, Deng S, et al. Hypoxanthine is a metabolic biomarker for inducing GSDME-dependent pyroptosis of endothelial cells during ischemic stroke [J]. Theranostics, 2024, 14(15): 6071-6087.
Protocol
| Cell experiment [1]: | |
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Cell lines |
Human HepG2 hepatic carcinoma cells |
|
Preparation Method |
HepG2 cells were seeded at a density of 1×104 cells/well in a black 96-well plate. Cells were incubated with a solution of 50μM DCFH-DA (2’,7’-dichlorofluorescein diacetate) in serum-free medium for 30min. This was followed by washing the cells twice with PBS. The cells were then incubated with Hypoxanthine for 2h. Intracellular ROS levels were determined using a fluorescence microscope. |
|
Reaction Conditions |
1.5, 2mM; 2h |
|
Applications |
Hypoxanthine induced ROS generation in HepG2 cells. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6J mice |
|
Preparation Method |
Adult C57BL/6J mice were received middle cerebral artery occlusion (MCAO) surgery. Intravenous injection of Hypoxanthine (135mg/kg) was carried out from one day before MCAO to one day after MCAO. Infarct volume assessment by triphenyl tetrazolium chloride (TTC) staining. |
|
Dosage form |
135mg/kg; 3d; i.v. |
|
Applications |
Hypoxanthine consistently increased the brain infarct volume. |
References: [1] Ryu H M, Kim Y J, Oh E J, et al. Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E-deficient mice and cells [J]. Journal of cellular and molecular medicine, 2016, 20(11): 2160-2172. [2] Ye J, Bi X, Deng S, et al. Hypoxanthine is a metabolic biomarker for inducing GSDME-dependent pyroptosis of endothelial cells during ischemic stroke [J]. Theranostics, 2024, 14(15): 6071-6087. | |
Product Documents
Purity:>99.50%Appearance:A solid
Chemical Properties
CAS No.
68-94-0
Synonyms
6-Hydroxypurine, NSC 14665, NSC 129419
SMILES
O=C1N=CNC2=C1NC=N2
Molecular Formula
C5H4N4O
Molecular Weight
136.11
Solubility
≥ 1.36mg/mL in DMSO with ultrasonic
Storage
Store at 2-8°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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