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D4476 (Synonyms: Casein Kinase I Inhibitor)

カタログ番号GC13202

CK1とALK5の阻害剤

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D4476 化学構造

Cas No.: 301836-43-1

サイズ 価格 在庫数 個数
10mM (in 1mL DMSO)
$69.00
在庫あり
10mg
$62.00
在庫あり
50mg
$178.00
在庫あり
200mg
$479.00
在庫あり
1g
$1,133.00
在庫あり

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Sample solution is provided at 25 µL, 10mM.

Description of D4476

D4476 is a relatively specific and cell-permeant inhibitor of CK1 and ALK5 with respective IC50 values of 0.3 and 0.5 μM at 0.1 mM ATP in vitro.[1]

CK1 (casein kinase 1) phosphorylates serine residues that are located close to another phosphoserine in multisite phosphorylation domains (MPDs). D4476 may be an ATP-competitive inhibitor of CK1. It weakly inhibits SAPK2a/p38 and does not inhibit PKB or SGK. In H4IIE cells, the site-specific phosphorylation (Ser322 and Ser325) of FOXO1a was inhibited at 50 and 125 μM, which reduced the initial rate of nuclear exclusion[1]. In Hela cells, the phosphorylation(Ser185) of RhoB caused by CK1 was inhibited by D4476 at 50 μM then the RhoB-dependent actin fiber formation was subsequently activated[2]. D4476 treatment has been observed to result in a partial p53-dependent growth arrest in HCT116 cells.[3]

References:
1. G. Rena, J. Bain, M. Elliott and P. Cohen, EMBO Rep 2004, 5, 60-65.
2. V. Tillement, I. Lajoie-Mazenc, A. Casanova, C. Froment, M. Penary, D. Tovar, R. Marquez, B. Monsarrat, G. Favre and A. Pradines, Exp Cell Res 2008, 314, 2811-2821.
3. A. S. Huart, N. J. MacLaine, D. W. Meek and T. R. Hupp, J Biol Chem 2009, 284, 32384-32394.

Protocol of D4476

Cell experiment [1,2]:

Cell lines

H4IIE, Hela and HCT116 cell lines

Preparation method

The solubility of this compound in DMSO is >19.9 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

H4IIE: 0-150 μM for 1 h; Hela: 50 μM; HCT116: 5-40 μM for 48 h

Applications

In H4IIE hepatoma cells, D4476 specifically inhibited the phosphorylation of endogenous forkhead box transcription factor O1a (FOXO1a) on Ser322 and Ser325 within its MPD, without affecting the phosphorylation of other sites. It was also found that although the treatment of in HCT116 cells with D4476 led to a partial p53-dependent growth arrest, the induction of p53-independent apoptosis by D4476 suggested a critical role for the MDM2-CK1 complex in maintaining E2F-1 anti-apoptotic signaling.

References:

[1] Rena G, et al. D4476, a cell-permeant inhibitor of CK1, suppresses the site-specific phosphorylation and nuclear exclusion of FOXO1a. EMBO Rep. 2004 Jan;5(1):60-5.

[2] Huart AS, MacLaine NJ, Meek DW, Hupp TR.CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability. J Biol Chem. 2009 Nov 20;284(47):32384-94.

Chemical Properties of D4476

Cas No. 301836-43-1 SDF
同義語 Casein Kinase I Inhibitor
Chemical Name 4-[4-(2,3-dihydro-1,4-benzodioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl]benzamide
Canonical SMILES C1COC2=C(O1)C=CC(=C2)C3=C(NC(=N3)C4=CC=C(C=C4)C(=O)N)C5=CC=CC=N5
Formula C23H18N4O3 M.Wt 398.41
溶解度 ≥ 19.9 mg/mL in DMSO, ≥ 41.5 mg/mL in EtOH with ultrasonic and warming Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of D4476

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.51 mL 12.5499 mL 25.0998 mL
5 mM 0.502 mL 2.51 mL 5.02 mL
10 mM 0.251 mL 1.255 mL 2.51 mL
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In vivo Formulation Calculator (Clear solution) of D4476

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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