DD1, a proteasome inhibitor, targets Bax activation and P70S6K degradation during acute myeloid leukemia (AML) apoptosis. DD1 induces apoptosis in the caspase-dependent manner.DD1 induces mitochondrial membrane depolarization and Bad dephosphorylation.
DD1 (20 μM; 96 h) shows antiproliferation activity in a dose- and time-dependent manner in U937 cells[1].DD1 induces cell cycle arrest at G2/M phase and apoptosis[1].DD1 (10, 20 μM; 48 h) induces mitochondrial membrane depolarization, Bax upregulation and Bad dephosphorylation[1].DD1 (2-20 μM; 40 h) shows inhibitor for cmotrypsin-like activity in U937 lysates[1].
References:
[1]. Marion Piedfer, et al. p70S6 kinase is a target of the novel proteasome inhibitor 3,3'-diamino-4'-methoxyflavone during apoptosis in human myeloid tumor cells. Biochim Biops Acta.2013 Jun;1833(6):1316-28.