Ac-DEVD-CHO |
Catalog No.GC32695
One-Click Copy Product Info
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Ac-DEVD-CHO es un inhibidor de Caspase-3 con un valor de IC50 de 0.016μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 169332-60-9
Sample solution is provided at 25 µL, 10mM.
Ac-DEVD-CHO es un inhibidor de Caspase-3 con un valor de IC50 de 0.016μM[1]. Ac-DEVD-CHO también tiene actividad inhibitoria contra otros miembros de la familia de la caspasa, pero es más selectivo para la caspase-3, con un valor Ki de 0.23nM [2]. Ac-DEVD-CHO inhibe el proceso de apoptosis al inhibir la actividad de las caspases, y se puede utilizar para estudiar el proceso de apoptosis y enfermedades relacionadas, como la degeneración de la retina[3].
In vitro, tratados osteoclasts con (OCLs)Ac-DEVD-CHO (10μM) durante 24 h, bloqueando parcialmente la apoptosis inducida por sinomenina y reduciendo el número de núcleos apoptóticos [4]. Ac-DEVD-CHO (100μM) trató las células musculares lisas vasculares durante 48 horas, reduciendo significativamente el nivel de expresión de caspase-3 e inhibiendo la apoptosis inducida por el artesunato[5].
In vivo, ratones tratados con Ac-DEVD-CHO (2mg/kg) con retinitis hereditaria a través de inyección intraperitoneal, inhibiendo efectivamente la apoptosis de las células fotorreceptoras y retrasando temporalmente la degeneración retiniana[6]. Ac-DEVD-CHO (4mg/kg) se inyectó por vía subcutánea en ratones con lesión renal aguda y redujo significativamente las concentraciones séricas de BUN, TNF-α, IL-6, IL-10 y la tasa de apoptosis de las células renales [7].
References:
[1] Firoozpour L, Gao L, Moghimi S, et al. Efficient synthesis, biological evaluation, and docking study of isatin based derivatives as caspase inhibitors[J]. Journal of enzyme inhibition and medicinal chemistry, 2020, 35(1): 1674-1684.
[2] Garcia-Calvo M, Peterson E P, Leiting B, et al. Inhibition of human caspases by peptide-based and macromolecular inhibitors[J]. Journal of Biological Chemistry, 1998, 273(49): 32608-32613.
[3] Yao K, Wang K, Xu W, et al. Caspase-3 and its inhibitor Ac-DEVD-CHO in rat lens epithelial cell apoptosis induced by hydrogen in vitro[J]. Chinese medical journal, 2003, 116(07): 1034-1038.
[4] He L, Li X, Zeng X, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation[J]. Acta Pharmacologica Sinica, 2014, 35(2): 203-210.
[5] Zhang J, Wang L, Chen H, et al. Effect of Caspase Inhibitor Ac-DEVD-CHO on Apoptosis of Vascular Smooth Muscle Cells Induced by Artesunate[J]. AIMS Bioengineering, 2014, 1(1): 13-24.
[6] Yoshizawa K, Kiuchi K, Nambu H, et al. Caspase-3 inhibitor transiently delays inherited retinal degeneration in C3H mice carrying the rd gene[J]. Graefe's archive for clinical and experimental ophthalmology, 2002, 240: 214-219.
[7] Liu L X, Hu Z J, Li Y, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice[J]. Zhongguo wei Zhong Bing ji jiu yi xue, 2010, 22(12): 736-739.
| Experimentos celulares [1]: | |
Líneas celulares | OCLs |
Método de preparación | OCLs se incubaron con RANKL y se trataron con 0.5 mmol/L SIN con o sin el inhibidor esoecífico de la caspase-3 Ac-DEVD-CHO (10μM) durante 24 h. |
Condiciones de reacción | 10μM; 24 h |
Áreas de aplicación | Ac-DEVD-CHO bloqueó parcialmente el efecto de la apoptosis inducida por SIN y redujo el número de núcleos apoptóticos. |
| Experimentos con animales [2]: | |
Modelos animales | Ratones C3H e ICR |
Método de preparación | A partir de los 8 días de edad, 40 C3H y 40 ICR recibieron una inyección i.p. injection de 2 mg/kg de peso corporal de Ac-DEVD-CHO o salina cada dos días.Diez ratones C3H e ICR por grupo fueron seleccionados al azar y aseesinados a los 13 días de edad, y los 10 ratones C3H e ICR restantes en cada grupo fueron asesinados a los 17 días. |
Forma de dosificación | 2mg/kg; i.p. |
Áreas de aplicación | Ac-DEVD-CHO retrasa transitoriamente la degeneración retiniana heredada en ratones C3H que llevan el gen rd. |
References: | |
| Cas No. | 169332-60-9 | SDF | |
| Canonical SMILES | N-Acetyl-Asp-Glu-Val-Asp-al | ||
| Formula | C20H30N4O11 | M.Wt | 502.47 |
| Solubility | Water : ≥ 50 mg/mL (99.51 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9902 mL | 9.9508 mL | 19.9017 mL |
| 5 mM | 398 μL | 1.9902 mL | 3.9803 mL |
| 10 mM | 199 μL | 995.1 μL | 1.9902 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)