Hydroxytyrosol (Synonyms: Ba2774, DOPET, 3Hydroxytyrosol) |
Catalog No.GN10326
One-Click Copy Product Info
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Hydroxytyrosol is a natural polyphenolic compound primarily found in olive leaves, possessing potent antioxidant activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 10597-60-1
Sample solution is provided at 25 µL, 10mM.
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Hydroxytyrosol is a natural polyphenolic compound primarily found in olive leaves, possessing potent antioxidant activity[1]. Hydroxytyrosol can effectively scavenge free radicals and protect cells from oxidative stress damage[2], and is commonly used in the development of nutraceuticals and in research on anticancer, antithrombotic, anti-arteriosclerotic, and lipid-modulating[3,4].
In vitro, SH-SY-5Y cells pretreated with Hydroxytyrosol (2, 5, and 10μM) for 24h followed by treatment with 45mM glucose for 6h exhibited a dose-dependent increase in cell viability[5]. MC3T3-E1 cells were treated with Hydroxytyrosol (10, 50, and 100μM) for 18 days, which dose-dependently promoted calcium deposition and osteoblast mineralization[6].
In vivo, oral administration of Hydroxytyrosol (50mg/kg/day) by gavage to C57BL/6 mice fed a high-fat diet (HFD) for 17 weeks significantly inhibited the HFD-induced increases in body weight and organ weights (liver, perirenal fat, epididymal fat) and significantly improved glucose tolerance (OGTT) results[7]. db/db mice were orally administered Hydroxytyrosol (10mg/kg/day) for 8 weeks, which significantly increased the expression levels of mitochondrial complex I, II, and IV subunits in the cerebral cortex[5].
References:
[1] MANNA C, DELLA RAGIONE F, CUCCIOLLA V, et al. Biological effects of hydroxytyrosol, a polyphenol from olive oil endowed with antioxidant activity[J]. Advances in Nutrition and Cancer 2, 1999: 115-130.
[2] RIETJENS S J, BAST A, HAENEN G R M M. New insights into controversies on the antioxidant potential of the olive oil antioxidant hydroxytyrosol[J]. Journal of Agricultural and Food Chemistry, 2007, 55(18): 7609-7614.
[3] BERTELLI M, KIANI A K, PAOLACCI S, et al. Hydroxytyrosol: a natural compound with promising pharmacological activities[J]. Journal of Biotechnology, 2020, 309: 29-33.
[4] ZHANG X, QIN Y, WAN X, et al. Hydroxytyrosol plays antiatherosclerotic effects through regulating lipid metabolism via inhibiting the p38 signal pathway[J]. BioMed Research International, 2020, 2020(1): 5036572.
[5] ZHENG A, LI H, XU J, et al. Hydroxytyrosol improves mitochondrial function and reduces oxidative stress in the brain of db/db mice: role of AMP-activated protein kinase activation[J]. British Journal of Nutrition, 2015, 113(11): 1667-1676.
[6] HAGIWARA K, GOTO T, ARAKI M, et al. Olive polyphenol hydroxytyrosol prevents bone loss[J]. European Journal of Pharmacology, 2011, 662(1-3): 78-84.
[7] CAO K, XU J, ZOU X, et al. Hydroxytyrosol prevents diet-induced metabolic syndrome and attenuates mitochondrial abnormalities in obese mice[J]. Free Radical Biology and Medicine, 2014, 67: 396-407.
| Cell experiment [1]: | |
Cell lines | SH-SY-5Y cells |
Preparation Method | SH-SY-5Y cells were pretreated with the indicated concentrations of Hydroxytyrosol (2, 5 and 10μM) for 24h, followed by a 6h treatment with 45mM glucose, and cell viability was then detected by the MTT method. |
Reaction Conditions | 2, 5 and 10μM; 24h |
Applications | Treatment with Hydroxytyrosol can dose-dependently increase cell viability. |
| Animal experiment [2]: | |
Animal models | HFD C57BL/6 mice |
Preparation Method | C57BL/6 mice fed a high-fat diet with a daily oral gavage of 50mg/kg/day Hydroxytyrosol for 17 weeks. Body weight was recorded weekly, and organ weights (liver, perirenal fat, and epididymal fat) were measured at the end of the experiment. For the OGTT, after an overnight fasting, glucose was orally administered at 1g/kg body weight, and blood samples were collected from the retrobulbar vein at 0, 30, 60, 120, and 180min post-administration to assess glucose tolerance. |
Dosage form | 50mg/kg/day; 17 weeks; i.g. |
Applications | Treatment with Hydroxytyrosol significantly inhibited high-fat diet-induced weight gain and organ weight (liver, perirenal fat, epididymal fat) increases, and significantly improved OGTT results. |
| Cas No. | 10597-60-1 | SDF | |
| Sinónimos | Ba2774, DOPET, 3Hydroxytyrosol | ||
| Chemical Name | 4-(2-hydroxyethyl)benzene-1,2-diol | ||
| Canonical SMILES | C1=CC(=C(C=C1CCO)O)O | ||
| Formula | C8H10O3 | M.Wt | 154.16 |
| Solubility | DMSO : 125 mg/mL (810.85 mM; Need ultrasonic); H2O : 100 mg/mL (648.68 mM; Need ultrasonic); Ethanol : 50 mg/mL (324.34 mM; Need ultrasonic) | Storage | Store at -20℃ |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.4868 mL | 32.4338 mL | 64.8677 mL |
| 5 mM | 1.2974 mL | 6.4868 mL | 12.9735 mL |
| 10 mM | 648.7 μL | 3.2434 mL | 6.4868 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)