GLPBio

2-Phospho-L-ascorbic acid trisodium salt

Catalog No.: GC35095Purity:>98.00%
COASDSData Sheet
La sal trisÓdica del Ácido 2-fosfo-L-ascÓrbico (trisodio del Ácido 2-fosfo-L-ascÓrbico) es un derivado de la vitamina C de acciÓn prolongada que puede estimular la formaciÓn y expresiÓn de colÁgeno.

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2-Phospho-L-ascorbic acid trisodium salt
Cas No.: 66170-10-3
10mM (in 1mL Water)In Stock
$45.00
1
500mgIn Stock
$36.00
1
1gIn Stock
$45.00
1
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Citas (Citations)

1 publicationsPublicaciones de investigadores que utilizan este producto

Descripción (Description)

2-Phospho-L-ascorbic acid trisodium salt, a stable form of Ascorbic acid (Asc), is frequently employed as source of Asc in cell culture[1]. 2-Phospho-L-ascorbic acid trisodium salt promotes osteoblast differentiation by enhancing collagen synthesis and alkaline phosphatase (ALP) activity[2]. 2-Phospho-L-ascorbic acid trisodium salt has been widely used as a substrate for detecting target IgG and for determining hexavalent tungsten (W(VI)) in water[3-4]. 2-Phospho-L-ascorbic acid trisodium salt has been widely used in the field of regenerative medicine to induce fibroblasts to produce extracellular matrix (ECM) for the preparation of tissue-engineered matrices[5].

In vitro, 2-Phospho-L-ascorbic acid trisodium salt treatment (300μM) for 2 weeks promoted the proliferation of human corneal endothelial cells (HCECs) and reduced the level of 8-OHdG in mitochondrial DNA[6]. Treatment with 100µM 2-Phospho-L-ascorbic acid trisodium salt for 3 weeks stimulated collagen production and promoted cell proliferation in skin fibroblasts[7].

References:
[1] Liang L, He M, Zhang Y, et al. Unraveling the 2, 3-diketo-l-gulonic acid-dependent and-independent impacts of l-ascorbic acid on somatic cell reprogramming[J]. Cell & Bioscience, 2023, 13(1): 218.
[2] Takamizawa S, Maehata Y, Imai K, et al. Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells[J]. Cell biology international, 2004, 28(4): 255-265.
[3] Pham X H, Hahm E, Kim T H, et al. Enzyme-catalyzed Ag growth on Au nanoparticle-assembled structure for highly sensitive colorimetric immunoassay[J]. Scientific Reports, 2018, 8(1): 6290.
[4] Alvarado-Gámez A L, Alonso-Lomillo M A, Domínguez-Renedo O, et al. A chronoamperometric screen printed carbon biosensor based on alkaline phosphatase inhibition for W (VI) determination in water, using 2-phospho-l-ascorbic acid trisodium salt as a substrate[J]. Sensors, 2015, 15(2): 2232-2243.
[5] Brownell D, Carignan L, Alavi R, et al. Impact of the use of 2-phospho-L ascorbic acid in the production of engineered stromal tissue for regenerative medicine[J]. Cells, 2025, 14(14): 1123.
[6] Shima N, Kimoto M, Yamaguchi M, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate[J]. Investigative ophthalmology & visual science, 2011, 52(12): 8711-8717.
[7] Hata R I, Senoo H. L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts[J]. Journal of cellular physiology, 1989, 138(1): 8-16.

Protocolo (Protocol)

Cell experiment [1]:

Cell lines

 Human forearm skin fibroblasts

Preparation Method

 Human forearm skin fibroblasts (5×104 cells/well) were plated onto 35-mm dishes and grown in DMEM medium supplemented with Fungizone (250µg/L), penicillin G (50mg/L), dihydrostreptomycin (50mg/L), N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (1g/L), and 10% fetal bovine serum (DMEM) in the absence or presence of 2-Phospho-L-ascorbic acid trisodium salt (0, 100, 200, 400, 600, 800, and 1000µM), 0.15mM magnesium chloride and 0.1mM sodium Lascorbate at 37°C for 3 weeks in an atmosphere of 5% CO2. Cell proliferation was measured.

Reaction Conditions

 0, 100, 200, 400, 600, 800, and 1000µM; 3 weeks

Applications

 2-Phospho-L-ascorbic acid trisodium salt treatment markedly enhanced cell proliferation of human forearm skin fibroblasts in a dose-dependent manner.
References:
[1] Hata R I, Senoo H. L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts[J]. Journal of cellular physiology, 1989, 138(1): 8-16.

Documentos del producto (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Propiedades químicas (Chemical Properties)

N.º CAS
66170-10-3
SMILES
O[C@@H](CO)[C@@H](O1)C(O[Na])=C(OP(O[Na])(O[Na])=O)C1=O
Fórmula molecular
C6H6Na3O9P
Peso molecular
322.05
Solubilidad
Water : 150 mg/mL (465.77 mM) DMSO : 6 mg/mL (18.63 mM)
Almacenamiento
Store at RT
Consejos generales
Seleccione un disolvente adecuado según los datos de solubilidad del producto. Una vez preparada, divida la solución madre en alícuotas para evitar ciclos repetidos de congelación y descongelación.
Utilice las soluciones madre en un plazo de 6 meses si se conservan a -80°C, o de 1 mes si se conservan a -20°C.
Para mejorar la solubilidad, caliente el tubo a 37°C y soníquelo durante un periodo adecuado.
Condiciones de envío
Las muestras de evaluación se envían con acumuladores de frío. Los demás tamaños se envían a temperatura ambiente o, previa solicitud, con acumuladores de frío.

Calculadoras (Calculators)Molaridad / Dilución / Peso molecular / Conversión de unidades / Formulación in vivo / Solubilidad

g/mol

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